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Review

How to Tackle Underdeterminacy in Metabolic Flux Analysis? A Tutorial and Critical Review

by 1,*,† and 2,*,†
1
3BIO-BioControl, Université Libre de Bruxelles, Avenue F.D. Roosevelt, 50-CP 165/61, 1050 Brussels, Belgium
2
Systems, Estimation, Control and Optimization (SECO), University of Mons, 7000 Mons, Belgium
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Andrzej Pawłowski
Received: 31 July 2021 / Revised: 26 August 2021 / Accepted: 27 August 2021 / Published: 2 September 2021
(This article belongs to the Special Issue Mathematical Modeling and Control of Bioprocesses)
Metabolic flux analysis is often (not to say almost always) faced with system underdeterminacy. Indeed, the linear algebraic system formed by the steady-state mass balance equations around the intracellular metabolites and the equality constraints related to the measurements of extracellular fluxes do not define a unique solution for the distribution of intracellular fluxes, but instead a set of solutions belonging to a convex polytope. Various methods have been proposed to tackle this underdeterminacy, including flux pathway analysis, flux balance analysis, flux variability analysis and sampling. These approaches are reviewed in this article and a toy example supports the discussion with illustrative numerical results. View Full-Text
Keywords: flux variability analysis; flux balance analysis; sampling; metabolic network; elementary flux modes flux variability analysis; flux balance analysis; sampling; metabolic network; elementary flux modes
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MDPI and ACS Style

Bogaerts, P.; Vande Wouwer, A. How to Tackle Underdeterminacy in Metabolic Flux Analysis? A Tutorial and Critical Review. Processes 2021, 9, 1577. https://0-doi-org.brum.beds.ac.uk/10.3390/pr9091577

AMA Style

Bogaerts P, Vande Wouwer A. How to Tackle Underdeterminacy in Metabolic Flux Analysis? A Tutorial and Critical Review. Processes. 2021; 9(9):1577. https://0-doi-org.brum.beds.ac.uk/10.3390/pr9091577

Chicago/Turabian Style

Bogaerts, Philippe, and Alain Vande Wouwer. 2021. "How to Tackle Underdeterminacy in Metabolic Flux Analysis? A Tutorial and Critical Review" Processes 9, no. 9: 1577. https://0-doi-org.brum.beds.ac.uk/10.3390/pr9091577

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