Chemical Profiling, Antioxidant, Antiproliferative, and Antibacterial Potentials of Chemically Characterized Extract of Citrullus colocynthis L. Seeds
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material and Extract Preparation
2.2. Antioxidant Activity
2.2.1. β-Carotene Bleaching Assay
2.2.2. DPPH Assay
2.3. Antiprolifertaive Activity
2.3.1. Cell Culture
2.3.2. Cell Viability Assay
2.4. Antibacterial Activity
2.5. Identification of Constituents by GC-MS Analysis
2.6. Statistical Analysis
3. Results and Discussion
3.1. Antiproliferative Effect of C. colocynthis Seeds
3.2. Antioxidant Effect
3.3. Antibacterial Activity
3.4. Chemical Analysis of C. colocynhtis Seed Extract
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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RT (min) | Compound | RI | Area (%) | Formula | Chemical Structure |
---|---|---|---|---|---|
3.80 | Hexanal | 801 | 4.54 | C6H12O | |
6.26 | Hexadienol | 916 | 3.61 | C6H10O | |
9.86 | Undecane | 1100 | 2.04 | C11 H24 | |
11.21 | 2,4-Nonadienal | 1187 | 2.34 | C9H14O | |
11.50 | 2,4-Decadienal | 1295 | 7.79 | C10H16O | |
12.46 | Tetradecane | 1400 | 3.29 | C14H30 | |
14.77 | Hexadecane | 1600 | 1.27 | C16H34 | |
16.91 | Methoxy cinnamic acid | 1700 | 1.28 | C10H10O3 | |
19.23 | Sulfurous acid | 1200 | 1.43 | C12H26 | |
20.21 | Ethyl2-octynoate | 1283 | 1.66 | C10H16O2 | |
21.17 | Linalool propanoate | 1337 | 14.29 | C13H22O2 | |
21.20 | Pentadecane | 1500 | 7.15 | C15H32 | |
21.71 | Nonadienal | 1381 | 15.39 | C13H24O2 | |
23.02 | Butylated hydroxy anisol | 1489 | 4.28 | C11H16O2 | |
Total | 70.36% |
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Bourhia, M.; Bouothmany, K.; Bakrim, H.; Hadrach, S.; Salamatullah, A.M.; Alzahrani, A.; Khalil Alyahya, H.; Albadr, N.A.; Gmouh, S.; Laglaoui, A.; et al. Chemical Profiling, Antioxidant, Antiproliferative, and Antibacterial Potentials of Chemically Characterized Extract of Citrullus colocynthis L. Seeds. Separations 2021, 8, 114. https://0-doi-org.brum.beds.ac.uk/10.3390/separations8080114
Bourhia M, Bouothmany K, Bakrim H, Hadrach S, Salamatullah AM, Alzahrani A, Khalil Alyahya H, Albadr NA, Gmouh S, Laglaoui A, et al. Chemical Profiling, Antioxidant, Antiproliferative, and Antibacterial Potentials of Chemically Characterized Extract of Citrullus colocynthis L. Seeds. Separations. 2021; 8(8):114. https://0-doi-org.brum.beds.ac.uk/10.3390/separations8080114
Chicago/Turabian StyleBourhia, Mohammed, Kaoutar Bouothmany, Hanane Bakrim, Safaa Hadrach, Ahmad Mohammad Salamatullah, Abdulhakeem Alzahrani, Heba Khalil Alyahya, Nawal A. Albadr, Said Gmouh, Amine Laglaoui, and et al. 2021. "Chemical Profiling, Antioxidant, Antiproliferative, and Antibacterial Potentials of Chemically Characterized Extract of Citrullus colocynthis L. Seeds" Separations 8, no. 8: 114. https://0-doi-org.brum.beds.ac.uk/10.3390/separations8080114