Study of DNA Interaction and Cytotoxicity Activity of Oxidovanadium(V) Complexes with ONO Donor Schiff Base Ligands
Abstract
:1. Introduction
2. Results and Discussion
2.1. Synthesis
2.2. Spectral Characteristics
2.2.1. IR Spectroscopy
2.2.2. Electronic Spectra
2.2.3. NMR Spectra
2.2.4. ESI Mass Spectra
2.3. Single-Crystal X-ray Crystallography of 1
2.4. DNA-Binding Studies
2.4.1. UV–Vis Absorption Studies
2.4.2. Circular Dichroism Studies
2.5. Cytotoxicity
2.6. Nuclear DAPI Staining Assay
3. Materials and Methods
3.1. Materials
3.2. Synthesis of Ligands
3.3. Synthesis of Oxidovanadium(V) Complexes
3.4. Single-Crystal X-ray Crystallography
3.5. DNA-Binding Experiments
3.5.1. UV–Vis Absorption Studies
3.5.2. Circular Dichroism Studies
3.6. Cytotoxicity Analysis through MTT Assay
3.7. Nuclear DAPI Staining Assay
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Parameter | Anion “a” | Anion “b” |
---|---|---|
V–O1 | 1.9152(9) | 1.8942(9) |
V–O2 | 1.9735(9) | 1.9191(9) |
V–O3 | 1.6219(10) | 1.6356(10) |
V–O4 | 1.6463(9) | 1.6473(10) |
V–N1 | 2.1658(11) | 2.1968(11) |
C7–N1 | 1.3033(17) | 1.2942(17) |
O1–V–O2 | 155.41(4) | 140.66(4) |
O3–V–O4 | 109.05(5) | 108.51(5) |
N1–V–O4 | 140.02(5) | 155.17(5) |
A | H | B | H...B | A...B | A–H...B |
---|---|---|---|---|---|
O5a | H1o | O4b | 1.825(12) | 2.6548(14) | 171.3(18) |
O5b | H2o | O4a | 1.830(15) | 2.6640(14) | 171.7(17) |
N3a | H1n | O2a | 2.466(13) | 3.2244(15) | 145.4(13) |
N3a | H1n | O3a | 2.224(12) | 2.9473(15) | 139.8(14) |
N3b | H2n | O3b | 1.918(12) | 2.7904(16) | 170.7(14) |
Complex | Binding Constants (Kb) (M−1) |
---|---|
1 | 2.81 × 104 |
2 | 2.35 × 104 |
H2L | 1.59 × 104 |
Compound | IC50 (μM) | |
---|---|---|
HT-29 | NIH-3T3 | |
1 | 8.56 ± 0.62 | 67.85 ± 5.48 |
2 | 9.09 ± 0.03 | 79.77 ± 4.00 |
H2L | 7.75 ± 0.53 | 69.32 ± 4.42 |
Formula | [C6H16N][C13H8N2O7V] |
---|---|
Molecular weight | 457.35 |
Crystal system | triclinic |
Space group | P1 |
a/Å | 10.8226(1) |
b/Å | 10.9399(2) |
c/Å | 17.3389(3) |
α/° | 79.412(1) |
β/° | 78.905(1) |
γ/° | 86.861(1) |
V/Å3 | 1979.82(5) |
Z | 4 |
Dc/g cm−3 | 1.534 |
μ/mm−1 | 4.621 |
Measured data | 46,958 |
Unique data | 7061 |
Observed data (I ≥ 2.0σ(I)) | 6958 |
No. parameters | 559 |
R, obs. data; all data | 0.025; 0.026 |
Rw, obs. data; all data | 0.072; 0.072 |
Range of residual electron | |
density peaks/eÅ−3 | −0.58–0.23 |
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Sahu, G.; Tiekink, E.R.T.; Dinda, R. Study of DNA Interaction and Cytotoxicity Activity of Oxidovanadium(V) Complexes with ONO Donor Schiff Base Ligands. Inorganics 2021, 9, 66. https://0-doi-org.brum.beds.ac.uk/10.3390/inorganics9090066
Sahu G, Tiekink ERT, Dinda R. Study of DNA Interaction and Cytotoxicity Activity of Oxidovanadium(V) Complexes with ONO Donor Schiff Base Ligands. Inorganics. 2021; 9(9):66. https://0-doi-org.brum.beds.ac.uk/10.3390/inorganics9090066
Chicago/Turabian StyleSahu, Gurunath, Edward R. T. Tiekink, and Rupam Dinda. 2021. "Study of DNA Interaction and Cytotoxicity Activity of Oxidovanadium(V) Complexes with ONO Donor Schiff Base Ligands" Inorganics 9, no. 9: 66. https://0-doi-org.brum.beds.ac.uk/10.3390/inorganics9090066