Next Article in Journal
Association between Periodontitis and Genetic Polymorphisms in Interleukins among Patients with Diabetes Mellitus
Previous Article in Journal
The Impact of Oral Health on Respiratory Viral Infection
Article

Effect of Butyric Acid in the Proliferation and Migration of Junctional Epithelium in the Progression of Periodontitis: An In Vitro Study

1
Division of Molecular Microbiology, Department of Microbiology, Iwate Medical University, 1-1-1 Idai-dori, Yahaba-Cho, Shiwa-Gun, Iwate 028-3694, Japan
2
Division of Periodontology, Department of Conservative Dentistry, School of Dentistry, Iwate Medical University, 1-3-27 Chuo-dori, Morioka, Iwate 020-8505, Japan
3
Department of Periodontology, School of Dentistry, Showa University, 2-1-1 Kitasenzoku, Ohta-ku, Tokyo 145-8515, Japan
4
Division of Anatomical and Cellular Pathology, Department of Pathology, Iwate Medical University, 1-1-1 Idai-dori, Yahaba-Cho, Shiwa-Gun, Iwate 028-3694, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Andrea Pilloni
Received: 9 March 2021 / Revised: 6 April 2021 / Accepted: 13 April 2021 / Published: 16 April 2021
Purpose: To elucidate the effects of butyric acid (BA), a metabolite of bacteria involved in periodontitis, and a possible enhancer of the junctional epithelial cells. Methods: A murine junctional epithelial cell line, JE-1, was used to assess the effects of sodium butyrate (NaB) as BA. Cell proliferation, migration and attachment were analyzed. Additionally, gene and promoter expression analysis was performed, i.e., cap analysis of gene expression (CAGE) and gene ontology (GO) term enrichment analysis. Results: NaB affected junctional epithelial cell proliferation, migration and attachment. A high concentration of NaB caused cell death and a low concentration tended to promote migration and adhesion. CAGE analysis revealed 75 upregulated and 96 downregulated genes in the cells after 0.2 mM NaB stimulation for 3 h. Regarding GO term enrichment, the genes upregulated >4-fold participated predominantly in cell migration and proliferation. The results of this study suggest that BA produced from periodontopathic bacteria is involved in periodontal tissue destruction at high concentrations. Furthermore, at low concentrations, BA potentially participates in periodontal disease progression by increasing proliferation, migration and attachment of the junctional epithelium and thereby increasing epithelial down-growth. View Full-Text
Keywords: adhesion molecules; butyric acid; junctional epithelium; periodontopathic bacteria adhesion molecules; butyric acid; junctional epithelium; periodontopathic bacteria
Show Figures

Figure 1

MDPI and ACS Style

Ishikawa, T.; Sasaki, D.; Aizawa, R.; Shimoyama, Y.; Yamamoto, M.; Irié, T.; Sasaki, M. Effect of Butyric Acid in the Proliferation and Migration of Junctional Epithelium in the Progression of Periodontitis: An In Vitro Study. Dent. J. 2021, 9, 44. https://0-doi-org.brum.beds.ac.uk/10.3390/dj9040044

AMA Style

Ishikawa T, Sasaki D, Aizawa R, Shimoyama Y, Yamamoto M, Irié T, Sasaki M. Effect of Butyric Acid in the Proliferation and Migration of Junctional Epithelium in the Progression of Periodontitis: An In Vitro Study. Dentistry Journal. 2021; 9(4):44. https://0-doi-org.brum.beds.ac.uk/10.3390/dj9040044

Chicago/Turabian Style

Ishikawa, Taichi, Daisuke Sasaki, Ryo Aizawa, Yu Shimoyama, Matsuo Yamamoto, Tarou Irié, and Minoru Sasaki. 2021. "Effect of Butyric Acid in the Proliferation and Migration of Junctional Epithelium in the Progression of Periodontitis: An In Vitro Study" Dentistry Journal 9, no. 4: 44. https://0-doi-org.brum.beds.ac.uk/10.3390/dj9040044

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop