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Article

Mesenchymal Stromal Cells Enhance Vascularization and Epithelialization within 7 Days after Gingival Augmentation with Collagen Matrices in Rabbits

1
Central Research Institute of Dentistry and Maxillofacial Surgery, 119991 Moscow, Russia
2
Strukov Department of Pathological Anatomy, Sechenov First Moscow State Medical University (Sechenov University), 119991 Moscow, Russia
3
Faculty of Dentistry, Sechenov First Moscow State Medical University (Sechenov University), 119435 Moscow, Russia
4
Department of Regenerative Technologies and Biofabrication, National Medical Research Radiological Center, 249036 Obninsk, Russia
5
Research and Educational Resource Center for Cellular Technologies, Peoples’ Friendship University of Russia, 117198 Moscow, Russia
*
Author to whom correspondence should be addressed.
Academic Editor: Marco Tatullo
Received: 19 July 2021 / Revised: 26 August 2021 / Accepted: 27 August 2021 / Published: 4 September 2021
(This article belongs to the Section Restorative Dentistry and Traumatology)
Soft gingival tissue deficiency remains a severe problem leading to postoperative recession, peri-implantitis, and bone resorption. The use of collagen matrices does not always lead to complete rebuilding of the gingiva volume. The application of mesenchymal stromal cells (MSCs) simultaneously with collagen materials represents a promising approach for the restoration of soft gingival tissues. However, short-term effects of MSCs-enriched collagen grafts after gingival augmentation have not yet been studied properly. Mucograft and Mucoderm matrices were implanted in rabbits (n = 12) simultaneously with the intraoperative injection of rabbit bone marrow-derived mesenchymal stromal cells (BM-MSCs) or without cells. Collagen matrices were implanted under the flap or by the surface technique without intentional primary closure. The samples were harvested seven days after implantation, histological staining with hematoxylin and eosin, and immunohistochemical staining for VEGF, IGF1, and TGF were performed. The use of Mucoderm led to better augmentation outcomes on day 7 compared with Mucograft (p < 0.0001). Gingival augmentation in combination with the local administration of BM-MSCs led to better regeneration of the soft gingival tissues independently of the type of implanted collagen matrices (p < 0.0001). Furthermore, injection of BM-MSCs significantly enhanced gingival vascularization and epithelization with a clear positive correlation between vascular growth and epithelial response. Administration of BM-MSCs in combination with various collagen materials may potentially improve gingiva regeneration. View Full-Text
Keywords: dentistry; gingiva; mesenchymal stromal cells; Mucoderm; Mucograft; stem cell transplantation; tissue engineering dentistry; gingiva; mesenchymal stromal cells; Mucoderm; Mucograft; stem cell transplantation; tissue engineering
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MDPI and ACS Style

Kulakov, A.; Kogan, E.; Brailovskaya, T.; Vedyaeva, A.; Zharkov, N.; Krasilnikova, O.; Krasheninnikov, M.; Baranovskii, D.; Rasulov, T.; Klabukov, I. Mesenchymal Stromal Cells Enhance Vascularization and Epithelialization within 7 Days after Gingival Augmentation with Collagen Matrices in Rabbits. Dent. J. 2021, 9, 101. https://0-doi-org.brum.beds.ac.uk/10.3390/dj9090101

AMA Style

Kulakov A, Kogan E, Brailovskaya T, Vedyaeva A, Zharkov N, Krasilnikova O, Krasheninnikov M, Baranovskii D, Rasulov T, Klabukov I. Mesenchymal Stromal Cells Enhance Vascularization and Epithelialization within 7 Days after Gingival Augmentation with Collagen Matrices in Rabbits. Dentistry Journal. 2021; 9(9):101. https://0-doi-org.brum.beds.ac.uk/10.3390/dj9090101

Chicago/Turabian Style

Kulakov, Anatoliy, Evgenia Kogan, Tatiana Brailovskaya, Anna Vedyaeva, Nickolay Zharkov, Olga Krasilnikova, Mikhail Krasheninnikov, Denis Baranovskii, Timur Rasulov, and Ilya Klabukov. 2021. "Mesenchymal Stromal Cells Enhance Vascularization and Epithelialization within 7 Days after Gingival Augmentation with Collagen Matrices in Rabbits" Dentistry Journal 9, no. 9: 101. https://0-doi-org.brum.beds.ac.uk/10.3390/dj9090101

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