Mesenchymal Stromal Cells Enhance Vascularization and Epithelialization within 7 Days after Gingival Augmentation with Collagen Matrices in Rabbits
Abstract
:1. Introduction
2. Materials and Methods
2.1. Laboratory Animals
2.2. Cell Culture
2.3. Collagen Matrices
2.4. Matrices Implantation and Postoperative Monitoring
2.5. Histological Examination
2.6. Immunohistochemistry
2.7. Microscopy
2.8. Statistics
3. Results
3.1. Clinical View of the Postoperative Condition of the Gingiva
3.2. Morphological Studies
3.3. Epithelialization Assessment
3.4. Angiogenesis Assessment
3.5. Fibroblast Response Assessment
3.6. Lymphoid Infiltration Assessment
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Group | Subgroups | |
---|---|---|
Without Cells (W) | Injection of MSCs (I) | |
SG (n = 3) | SGW Subgroup: Surface augmentation (S) Mucograft (G) | SGI Subgroup: Surface augmentation (S) Mucograft (G) |
SD (n = 3) | SDW Subgroup: Surface augmentation (S) Mucoderm (D) | SDI Subgroup: Surface augmentation (S) Mucoderm (D) |
UG (n = 3) | UGW Subgroup: Augmentation under the flap (U) Mucograft (G) | UGI Subgroup: Augmentation under the flap (U) Mucograft (G) |
UD (n = 3) | UDW Subgroup: Augmentation under the flap (U) Mucoderm (D) | UDI Subgroup: Augmentation under the flap (U) Mucoderm (D) |
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Kulakov, A.; Kogan, E.; Brailovskaya, T.; Vedyaeva, A.; Zharkov, N.; Krasilnikova, O.; Krasheninnikov, M.; Baranovskii, D.; Rasulov, T.; Klabukov, I. Mesenchymal Stromal Cells Enhance Vascularization and Epithelialization within 7 Days after Gingival Augmentation with Collagen Matrices in Rabbits. Dent. J. 2021, 9, 101. https://0-doi-org.brum.beds.ac.uk/10.3390/dj9090101
Kulakov A, Kogan E, Brailovskaya T, Vedyaeva A, Zharkov N, Krasilnikova O, Krasheninnikov M, Baranovskii D, Rasulov T, Klabukov I. Mesenchymal Stromal Cells Enhance Vascularization and Epithelialization within 7 Days after Gingival Augmentation with Collagen Matrices in Rabbits. Dentistry Journal. 2021; 9(9):101. https://0-doi-org.brum.beds.ac.uk/10.3390/dj9090101
Chicago/Turabian StyleKulakov, Anatoliy, Evgenia Kogan, Tatiana Brailovskaya, Anna Vedyaeva, Nickolay Zharkov, Olga Krasilnikova, Mikhail Krasheninnikov, Denis Baranovskii, Timur Rasulov, and Ilya Klabukov. 2021. "Mesenchymal Stromal Cells Enhance Vascularization and Epithelialization within 7 Days after Gingival Augmentation with Collagen Matrices in Rabbits" Dentistry Journal 9, no. 9: 101. https://0-doi-org.brum.beds.ac.uk/10.3390/dj9090101