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Microbes: Food for the Future
Article

Combination of Synergic Enzymes and Ultrasounds as an Effective Pretreatment Process to Break Microalgal Cell Wall and Enhance Algal Oil Extraction

Healthy-Lipids Group, Departmental Section of Food Sciences, Faculty of Sciences, Universidad Autónoma de Madrid, 28049 Madrid, Spain
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Author to whom correspondence should be addressed.
Academic Editors: Tomas Lafarga and Francisco Gabriel Acién Fernández
Received: 29 June 2021 / Revised: 13 August 2021 / Accepted: 17 August 2021 / Published: 19 August 2021
Microalgal biomass is a sustainable source of bioactive lipids with omega-3 fatty acids. The efficient extraction of neutral and polar lipids from microalgae requires alternative extraction methods, frequently combined with biomass pretreatment. In this work, a combined ultrasound and enzymatic process using commercial enzymes Viscozyme, Celluclast, and Alcalase was optimized as a pretreatment method for Nannochloropsis gaditana, where the Folch method was used for lipid extraction. Significant differences were observed among the used enzymatic pretreatments, combined with ultrasound bath or probe-type sonication. To further optimize this method, ranges of temperatures (35, 45, and 55 °C) and pH (4, 5, and 8) were tested, and enzymes were combined at the best conditions. Subsequently, simultaneous use of three hydrolytic enzymes rendered oil yields of nearly 29%, showing a synergic effect. To compare enzymatic pretreatments, neutral and polar lipids distribution of Nannochloropsis was determined by HPLC–ELSD. The highest polar lipids content was achieved employing ultrasound-assisted enzymatic pretreatment (55 °C and 6 h), whereas the highest glycolipid (44.54%) and PE (2.91%) contents were achieved using Viscozyme versus other enzymes. The method was applied to other microalgae showing the potential of the optimized process as a practical alternative to produce valuable lipids for nutraceutical applications. View Full-Text
Keywords: microalgae; enzymatic pretreatment; Viscozyme®; Alcalase®; Celluclast®; Nannochloropsis gaditana; polar lipids; sonication microalgae; enzymatic pretreatment; Viscozyme®; Alcalase®; Celluclast®; Nannochloropsis gaditana; polar lipids; sonication
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MDPI and ACS Style

Blanco-Llamero, C.; García-García, P.; Señoráns, F.J. Combination of Synergic Enzymes and Ultrasounds as an Effective Pretreatment Process to Break Microalgal Cell Wall and Enhance Algal Oil Extraction. Foods 2021, 10, 1928. https://0-doi-org.brum.beds.ac.uk/10.3390/foods10081928

AMA Style

Blanco-Llamero C, García-García P, Señoráns FJ. Combination of Synergic Enzymes and Ultrasounds as an Effective Pretreatment Process to Break Microalgal Cell Wall and Enhance Algal Oil Extraction. Foods. 2021; 10(8):1928. https://0-doi-org.brum.beds.ac.uk/10.3390/foods10081928

Chicago/Turabian Style

Blanco-Llamero, Cristina, Paz García-García, and Francisco J. Señoráns 2021. "Combination of Synergic Enzymes and Ultrasounds as an Effective Pretreatment Process to Break Microalgal Cell Wall and Enhance Algal Oil Extraction" Foods 10, no. 8: 1928. https://0-doi-org.brum.beds.ac.uk/10.3390/foods10081928

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