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Article

Amelioration of Smoke Taint in Cabernet Sauvignon Wine via Post-Harvest Ozonation of Grapes

by
Margherita Modesti
1,2,*,
Colleen Szeto
2,3,
Renata Ristic
2,3,
WenWen Jiang
4,
Julie Culbert
4,
Cesare Catelli
5,
Fabio Mencarelli
6,
Pietro Tonutti
1 and
Kerry Wilkinson
2,3,*
1
Life Sciences Institute, Scuola Superiore Sant’Anna, Piazza Martiri della Libertà 33, 5612 Pisa, Italy
2
Department of Wine Science, Waite Research Institute, The University of Adelaide, PMB 1, Glen Osmond, SA 5064, Australia
3
The Australian Research Council Training Centre for Innovative Wine Production, PMB 1, Glen Osmond, SA 5064, Australia
4
The Australian Wine Research Institute, P.O. Box 197, Glen Osmond, SA 5064, Australia
5
P.C. di Pompeo Catelli S.R.L., Via Roma 81, Uggiate Trevano, 22029 Como, Italy
6
Department of Agriculture Food and Environment, University of Pisa, Via del Borghetto 80, 56124 Pisa, Italy
*
Authors to whom correspondence should be addressed.
Beverages 2021, 7(3), 44; https://0-doi-org.brum.beds.ac.uk/10.3390/beverages7030044
Submission received: 21 May 2021 / Revised: 17 June 2021 / Accepted: 25 June 2021 / Published: 1 July 2021
(This article belongs to the Special Issue Featured Papers in Wine, Spirits and Oenological Products Section)
Browse Figures
Versions Notes

Abstract

:
Strategies that mitigate the negative effects of vineyard exposure to smoke on wine composition and sensory properties are needed to address the recurring incidence of bushfires in or near wine regions. Recent research demonstrated the potential for post-harvest ozonation of moderately smoke-exposed grapes to reduce both the concentration of smoke taint marker compounds (i.e., volatile phenols and their glycosides) and the perceived intensity of smoke taint in wine, depending on the dose and duration of ozone treatment. The current study further evaluated the efficacy of ozonation as a method for the amelioration of smoke taint in wine by comparing the chemical and sensory consequences of post-harvest ozonation (at 1 ppm for 24 h) of Cabernet Sauvignon grapes following grapevine exposure to dense smoke, i.e., ozone treatment of more heavily tainted grapes. Ozonation again yielded significant reductions in the concentration of free and glycosylated volatile phenols—up to 25% and 30%, respectively. However, although the intensities of smoke-related sensory attributes were generally lower in wines made with smoke-exposed grapes that were ozonated (compared to wines made with smoke-exposed grapes that were not ozonated), the results were not statistically significant. This suggests that the efficacy of ozone treatment depends on the extent to which grapes have been tainted by smoke.

Graphical Abstract

1. Introduction

Globally, the occurrence of bushfires in or near prominent wine regions is increasing due to warmer and drier environmental conditions that are attributable to climate change [1,2,3]. This is particularly concerning for grape and wine producers because grapevine exposure to smoke can adversely affect grape and wine composition [4,5,6,7,8,9], in some instances leading to a perceptible taint characterized by smoky, medicinal, drying, and/or ashy characters [4,8,10]. The intensity of “smoke taint” in wine depends on the timing and duration of smoke exposure [11,12], as well as smoke density [13], i.e., factors that influence the extent to which smoke-derived volatile compounds can be adsorbed by grapes. These volatiles include phenols, such as guaiacol, o-, m-, and p-cresol, and syringol, which are found in smoke as a consequence of the thermal degradation of lignin that occurs when plant material is burned [14]. Volatile phenols have also been found in smoke-affected grapes and wines made from smoke-affected grapes [4,5,6,7,8,9,10,11,12,13,15]. As such, they have become useful markers for the detection and/or quantification of smoke taint. However, several studies have demonstrated that following grapevine exposure to smoke, volatile phenols accumulate in leaves and fruit in glycosylated forms [13,16,17,18,19,20]. Glycosylation of volatile phenols by glucosyltransferase enzymes increases their solubility and stability, and likely occurs to mitigate any toxic effects or risk of cellular damage [21]. Volatile phenol glycosides are odorless, but during fermentation, they can be metabolized by yeast and enzymes to release volatile phenols [4,7,8,20], which can then impart smoke-related sensory attributes in wine. A significant pool of volatile phenol glycosides typically remains in wines made from smoke-affected grapes [7,8,13,18,20] and may also contribute to the sensory perception of smoke taint through in-mouth hydrolysis by salivary enzymes [22]. Analytical methods have therefore been developed to quantify volatile phenol glycosides [17,18,20,23,24], so they can also be used as markers of smoke taint.
Researchers have quantified volatile phenols (in free and glycosylated forms) to evaluate the efficacy of methods that mitigate or ameliorate the impacts of grapevine smoke exposure. To date, there has been limited success with strategies that aim to reduce either the uptake of smoke in the vineyard [13,19,25,26] or the extraction of smoke taint compounds from grapes during winemaking [27]. Thus, for now, the most effective remediation strategies involve removal of smoke volatiles from tainted wine using adsorbent materials, such as activated carbon, which can either be added directly to wine [28] or used in combination with nanofiltration [29]. However, recent research has shown that post-harvest ozone (O3) treatment of smoke-exposed grapes can also reduce the concentration of volatile phenols (and their glycosides) in wine and the sensory perception of smoke taint [30,31].
Ozonation is often employed in food and beverage production as a sanitizing agent, but is known to stimulate biological responses, such as the biosynthesis of phenolic compounds, in different fruits and vegetables [32] and, specifically, in wine and table grapes [33,34]. In various crops, including grapes, the oxidation potential of O3 has been exploited to reduce the concentration of pesticide residues [35,36], to extend shelf life, [37], to control microbial growth [38,39], or to enhance polyphenol and/or volatile concentrations [40,41,42], albeit the outcome of O3 treatment can depend on the duration of exposure, the dose rate, and the method of application. When moderately smoke-affected Merlot grapes were exposed to 3 ppm of O3 for 12 h (post-harvest), no significant differences were observed in the volatile phenol or volatile phenol glycoside concentrations of the resulting wines [31]. However, a small but significant reduction in free and glycosylated volatile phenol concentrations was observed following post-harvest ozonation of grapes at 1 ppm for 24 h, and most importantly, the intensity of smoke-related wine sensory attributes was significantly diminished [31]. The current study aimed to further evaluate the efficacy of post-harvest ozonation as a method for amelioration of smoke taint in wine. It builds on previous research by comparing the chemical and sensory outcomes of O3 treatment of smoke-affected grapes from a different cultivar (i.e., Cabernet Sauvignon) and following exposure to dense smoke (i.e., more heavily tainted grapes).

2. Materials and Methods

2.1. Smoke Exposure of Grapevines

Cabernet Sauvignon grapevines (Vitis vinifera L.) grown in a vineyard on the University of Adelaide’s Waite Campus (in Urrbrae, SA, Australia, 34°58′ S, 138°38′ E) were exposed to smoke for 1 h (at ~7 days post-veraison) during the 2018/2019 growing season. Smoke treatments were applied to six adjacent vines (three vines at a time) using a purpose-built smoke tent (2.0 m × 6.0 m × 2.5 m) and commercial smokers, which have been described previously [13,31]; barley straw (~5 kg in total) was burned throughout the treatment to maintain smoke production. Control (unsmoked) vines were separated from the smoke-exposed vines by at least one panel of buffer vines. The vineyard management was previously reported [13], but, briefly: vines were planted in 1998 in north–south-aligned rows on their own roots and were trained to a bilateral cordon with a vertical shoot-positioned trellis system; they were hand-pruned (two nodes per spur) and drip irrigated (twice per week between fruit set and harvest).

2.2. Post-Harvest Ozonation of Grapes

Fruit (~12 kg per treatment) was handpicked from the control and smoke-exposed grapevines when maturity sampling indicated that the total soluble solids (TSSs) were 21° Brix (approximately four weeks after smoke exposure), as determined with an Atago digital refractometer (Tokyo, Japan). Control and smoke-exposed grapes were then randomly divided into six fruit parcels (~2 kg each). Three parcels each of control and smoke-exposed fruit (arranged as a single layer of bunches) were treated with 1 ppm of gaseous O3 (produced with an A series ozone generator, P.C. di Pompeo Catelli SRL, Uggiate-Trevano, Italy) for 24 h in a 4 °C cold room—hereafter, “control + O3” and “smoke + O3” treatments, respectively. The dose and duration of ozonation were determined according to conditions employed in previous studies involving ozonation of wine grapes [30,34,40]. The three remaining parcels of control and smoke-exposed fruit were also stored in the 4 °C cold room for 24 h, but without ozonation—hereafter, “control” and “smoke” treatments, respectively. Following ozonation, fruit parcels were utilized in small-scale winemaking, with the three fruit replicates from each treatment becoming wine replicates.

2.3. Small-Scale Winemaking

Grape bunches (~2 kg per replicate, per treatment) were destemmed and crushed by hand, after which 50 mg/kg of sulfur dioxide was added to the resulting must (as an 8% solution of potassium metabisulfite). The must pH was then adjusted to 3.5 with the addition of tartaric acid before addition of 100 mg/L of diammonium phosphate and inoculation with 150 mg/L of PDM yeast (Maurivin, AB Biotek, Sydney, NSW, Australia). Musts were fermented on skins at ambient temperature (~24 °C) for seven days, during which time the cap was plunged twice per day. When the wines approached dryness (i.e., at ~2 g/L of residual sugar), they were pressed, and fermentation continued at ambient temperature (~24 °C) until residual sugars were <1 g/L (determined enzymatically using a glucose/fructose test kit; Vintessential Laboratories Pty. Ltd., Dromana, VIC, Australia). Wines were then racked from gross lees and cold stabilized at 0 °C for 4 weeks before being bottled in 375 mL glass bottles with screw-cap closures. Prior to bottling, wine pH and free SO2 were adjusted to 3.5 and 20 mg/L, respectively (again via addition of tartaric acid and potassium metabisulfite), and samples were collected for chemical analysis. Wines were cellared (at 15 °C) for two months prior to sensory analysis.

2.4. Chemical Analysis of Wine

Chemical analyses were performed on wine replicates from each experimental treatment. Wine pH and titratable acidity (TA, expressed as g/L of tartaric acid) were measured using a Mettler Toledo T50 autotitrator coupled to a Mettler Toledo InMotion Flex autosampler (Port Melbourne, VIC, Australia). Ethanol content (as % alcohol by volume, abv) was measured with an alcolyzer (Anton Paar, Graz, Austria). Wine color density and hue were measured with an Infinite® 200 PRO spectrophotometer (Tecan, Männedorf, Switzerland) using the modified Somers color assay [43].
The concentrations of volatile phenols (guaiacol, 4-methylguaiacol, o-, m-, and p-cresol, syringol, and 4-methylsyringol) were measured in wine by the Australian Wine Research Institute’s Commercial Services laboratory, using gas chromatography–mass spectrometry and stable isotope dilution analysis (SIDA) methods reported previously [6,44]. The preparation of isotopically labeled standards (d3-guaiacol, d3-4-methylguaiacol, d7-o-cresol, and d3-syringol), method validation, and instrument operating conditions are provided in these publications. The limit of quantitation for volatile phenols was 1–2 µg/L. The concentrations of volatile phenol glycosides were measured in wine (as syringol glucose–glucoside (gentiobioside) equivalents) using previously reported liquid chromatography–tandem mass spectrometry and SIDA methods [6,24]. Again, the preparation of the isotopically labeled standard (d3-syringol gentiobioside), method validation, and instrument operating conditions are provided in these publications. The limit of quantitation for volatile phenol glycosides was 1 µg/L.

2.5. Sensory Analysis of Wine

Wine sensory profiles were determined by descriptive analysis (DA) [45] with a panel of 15 judges (10 female and 5 male, aged 25 to 53 years) comprising staff and students from the University of Adelaide and the Australian Wine Research Institute. Panelists were recruited based on their availability and prior involvement in DA of smoke-tainted wines; nine panelists had >10 years of experience in sensory analysis of smoke-tainted wines.
The panel evaluated 18 attributes generated in previous smoke taint studies, e.g., [8,46,47], comprising: fruit, smoke, cold ash, earthy, burnt rubber, and medicinal aromas; fruit, smoky, and medicinal flavors; ashy, woody, drying, and metallic aftertastes; bitterness, acidity, hotness, astringency, and body. Sensory assessments were performed in individual sensory booths under controlled environmental conditions (i.e., ventilation, lighting, and a constant temperature of 22–23°C). Aliquots (30 mL) of the 12 wines were served in covered, 3-digit coded stemmed wine glasses, presented monadically using a randomized presentation order (across panelists). To minimize sensory fatigue, 1 min breaks were enforced between samples, with a 3 min break enforced after four samples. Distilled water and plain crackers were provided to panelists as palate cleansers. Panelists were asked to rate the intensity of sensory attributes using unstructured line scales, with “low” and “high” anchors placed at 10% and 90% of the scale, respectively. Data were acquired with the RedJade software (Redwood Shores, CA, USA).

2.6. Statistical Analysis

Chemical data were analyzed with one-way analysis of variance (ANOVA) using GenStat (19th Edition, VSN International Limited, Herts, UK). Mean comparisons were performed with the least significant difference (LSD) multiple comparison test at p < 0.05. Sensory data were analyzed with two-way ANOVA, with the judges treated as a random effect and the samples as a fixed effect, by using SenPAQ (version 5.01, Qi Statistics, Reading, UK) and XLSTAT (version 2018.1.1, Addinsoft, New York, NY, USA). Again, mean comparisons were performed with the LSD multiple comparison test at p < 0.05.

3. Results and Discussion

3.1. Influence of Post-Harvest Ozonation on Wine Composition

The compositional consequences of grapevine exposure to smoke and post-harvest ozonation of grapes were determined by comparing the volatile phenol and volatile phenol glycoside concentrations of finished wines (Table 1 and Table S1). Guaiacol and syringol were the only volatile phenols detected in the control wines (at 1 and ~2 µg/L, respectively), along with low levels of volatile phenol glycosides (i.e., ≤13.4 µg/L); ozonation of the control grapes had no significant effect on the free or glycosylated volatile phenol concentrations of the control wines. In contrast, the concentrations of volatile phenols and their glycosides were elevated in wines made from smoke-exposed grapes, and ozonation of smoke-exposed grapes did significantly affect the concentrations of many of the smoke taint marker compounds measured (Table 1 and Table S1).
Guaiacol and o- and m-cresols were the most abundant smoke-derived volatile phenols detected in free (aglycone) forms (at up to 30 and 8.3 µg/L, respectively), while guaiacol and syringol were the most abundant glycosylated volatile phenols (at up to 340 and 614 µg/L, respectively). The relative abundance of these compounds in wines made from smoke-exposed grapes was consistent with previous research [8,13,31,48], but importantly, the concentrations detected confirmed that a high degree of smoke taint was achieved, as intended, i.e., the volatile phenol and/or volatile phenol glycoside concentrations were comparable to levels reported for wines made from grapes exposed to dense smoke [13] or wines deemed to be heavily smoke tainted by sensory analysis [8,13].
Ozonation of smoke-exposed grapes resulted in wines with significantly lower concentrations of guaiacol, 4-methylguaiacol, and syringol (i.e., ~16–23% decreases, Table 1) and most of the volatile phenol glycosides that were measured (i.e., ~12–31% decreases, Table S1), the exceptions being guaiacol glucose–glucoside and phenol glucoside, for which the concentrations did not significantly change. These results further demonstrate that post-harvest ozonation of grapes can mitigate the compositional effects of grapevine smoke exposure. However, where grapes are heavily tainted by smoke (as in the current study), a substantial proportion of smoke taint marker compounds remain in the resulting wine and might therefore still impart perceivable smoke characters.
Some small but statistically significant differences in basic chemistry measurements were also observed amongst the wines (Table 2). There were no differences in pH, but the control wine made from grapes without O3 treatment had a significantly higher TA than other wines. This wine also had a significantly higher alcohol content; however, differences in alcohol content were not considered to be a consequence of either ozonation or smoke exposure. Grapevine exposure to smoke can affect sugar accumulation in grapes, but has only been observed where vines were repeatedly exposed to smoke during a single growing season [11]. The differences in TA and alcohol were instead attributed to variation in fruit maturity arising from phenological differences amongst vines, as reported in an earlier study [13] that was based in the same vineyard. Previous research has demonstrated that ozonation of grapes can affect the anthocyanin concentrations of juice [49] and wine [40]. In the current study, the color density of the control wine was significantly higher and its color hue was significantly lower than those observed for other wines (Table 2). The potential for differences in wine color to influence sensory analysis was therefore addressed by presenting wines to the DA panel monadically (i.e., one at a time).

3.2. Influence of Post-Harvest Ozonation on Wine Sensory Profiles

The sensory profiles of wines made from control or smoke-exposed grapes and with or without ozonation (1 ppm for 24 h) are shown in Figure 1. The DA panel did not perceive any significant differences between the two control wines (i.e., due to ozonation). These wines both exhibited fruit aromas and flavors, and there were no apparent smoky or ashy characters (ratings for smoke-related attributes were low, i.e., ≤1.8, Table S2); a drying aftertaste was perceived (Figure 1, Table S2), but was rated similarly in all wines and was therefore not considered to reflect either smoke exposure or O3 treatment. In contrast, the wines made with smoke-exposed grapes exhibited diminished fruit aromas and flavors, as well as perceivable smoke, cold ash, burnt rubber, and medicinal aromas, smoky and medicinal flavors, and an ashy aftertaste (Figure 1, Table S2), i.e., sensory attributes indicative of smoke taint [4,8,10,13].
The mean intensity ratings given to smoke-related sensory attributes—smoke and cold ash aromas, smoky and medicinal flavors, and ashy aftertaste, in particular—were generally lower for wines made from smoke-exposed grapes that were treated with O3 post-harvest than for wines made from smoke-exposed grapes that were not ozonated (Figure 1, Table S2). However, these ratings were not statistically significant. Whereas post-harvest ozonation of moderately smoke-affected Merlot grapes (at 1 ppm for 24 h) significantly improved wine sensory properties—i.e., fruit characters were enhanced and smoke attributes diminished [31]—the Cabernet Sauvignon grapes treated in the current study were more heavily tainted (due to exposure to dense smoke achieved by deliberately burning more fuel). As such, despite achieving a significant reduction in free and glycosylated volatile phenol concentrations using the same O3 treatment (i.e., 1 ppm for 24 h; Table 1), the resulting wine still exhibited a perceptible taint (Figure 1). This suggests that the efficacy of O3 treatment depends on the degree to which grapes are tainted by smoke and that ozonation might only be suitable for amelioration of grapes with low to moderate levels of smoke taint. Where smoke exposure occurs immediately prior to harvest, ozone treatment might still offer an effective approach to the remediation of smoke taint, given that volatile phenols appear to be more susceptible to the effects of ozonation than their glycosides [31].

4. Conclusions

Smoke taint will remain a challenge for grapegrowers and wine producers while bushfires continue to occur in or near wine regions during the annual growing season. As such, strategies are needed to mitigate or ameliorate the impacts of grapevine smoke exposure. Post-harvest ozonation can be employed to remediate smoke-exposed grapes, but findings from the current study suggest that the efficacy of O3 treatment might be limited according to how heavily grapes are tainted by smoke. Ozonation might therefore be effective for the remediation of grapes with low to moderate levels of taint, but in the case of more heavily tainted grapes, the resulting wine might still exhibit a perceivable taint and require further amelioration using methods that remove smoke taint marker compounds from wine (e.g., nanofiltration and/or adsorbents, such as activated carbon).

Supplementary Materials

The following are available online at https://0-www-mdpi-com.brum.beds.ac.uk/article/10.3390/beverages7030044/s1, Table S1: Concentrations (µg/L) of volatile phenol glycosides in wines made with control or smoke-exposed grapes, with or without post-harvest ozone treatment (1 ppm for 24 h), Table S2: Mean intensity ratings for sensory attributes of wines made with control or smoke-exposed grapes, with or without post-harvest ozone treatment (1 ppm for 24 h).

Author Contributions

All authors were involved in the conceptualization; methodology, M.M., C.S., R.R., W.J., J.C., and C.C.; resources, C.C., F.M., and K.W.; data curation, M.M., C.S., R.R., and K.W.; writing—original draft preparation, M.M. and K.W.; writing—review and editing, all authors; supervision, F.M., K.W., and P.T. All authors have read and agreed to the published version of the manuscript.

Funding

M.M. was funded as part of a PhD program in Agrobiosciences at the Life Sciences Institute in the Sant’Anna School of Advanced Study (Pisa, Italy). C.S. was funded by the Australian Research Council Training Centre for Innovative Wine Production (www.arcwinecentre.org.au), which is funded as part of the ARC’s Industrial Transformation Research Program (Project No. IC170100008), with support from Wine Australian and industry partners. R.R. was supported by Australian grapegrowers and winemakers through their investment body, Wine Australia, with matching funds from the Australian Government. J.C and W.J. were supported by the AWRI and through funding from the Australian Government Department of Agriculture, Water, and the Environment as part of its Rural R&D for Profit program and Wine Australia.

Institutional Review Board Statement

This study was approved by the Human Research Ethics Committee of the University of Adelaide (H-2019-095).

Informed Consent Statement

DA panelists gave informed consent before participating in this study.

Data Availability Statement

The data presented in this study are available on request from the corresponding author (pending privacy and ethical considerations).

Acknowledgments

The authors gratefully acknowledge the AWRIs Commercial Services Laboratory for the GC-MS analysis of wine, as well as the University of Adelaide and AWRI staff and students who participated in the sensory analysis. Special thanks is also given to Andrea Bellincontro (Tuscia University, Viterbo, Italy), who contributed to the development of the ozone application methods.

Conflicts of Interest

The authors declare no conflict of interest. The funding bodies had no role in the design of the study, in the collection, analyses, or interpretation of data, in the writing of the manuscript, or in the decision to publish the results.

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Beverages 07 00044 g001 550
Figure 1. Sensory profiles of Cabernet Sauvignon wines made with control or smoke-exposed grapes, with or without post-harvest ozone treatment (1 ppm for 24 h). A = aroma; F = flavor; AT = aftertaste. Data are mean ratings from three wine replicates, each evaluated by 15 judges; * indicates statistical significance (p = 0.05, two-way ANOVA).
Figure 1. Sensory profiles of Cabernet Sauvignon wines made with control or smoke-exposed grapes, with or without post-harvest ozone treatment (1 ppm for 24 h). A = aroma; F = flavor; AT = aftertaste. Data are mean ratings from three wine replicates, each evaluated by 15 judges; * indicates statistical significance (p = 0.05, two-way ANOVA).
Beverages 07 00044 g001
Table
Table 1. Concentrations (µg/L) of volatile phenols and volatile phenol glycosides in Cabernet Sauvignon wines made with control or smoke-exposed grapes, with or without post-harvest ozone treatment (1 ppm for 24 h).
Table 1. Concentrations (µg/L) of volatile phenols and volatile phenol glycosides in Cabernet Sauvignon wines made with control or smoke-exposed grapes, with or without post-harvest ozone treatment (1 ppm for 24 h).
ControlControl + O3SmokeSmoke + O3p
guaiacoltr1.0 ± 1.0 c30 ± 4.5 a23 ± 1.5 b<0.001
4-methylguaiacolndnd4.3 ± 0.6 a3.3 ± 0.6 b<0.001
o-cresolndnd8.3 ± 1.57.0 ± 0.01ns
m-cresolndnd8.3 ± 1.57.0 ± 0.01ns
p-cresolndnd4.0 ± 0.014.0 ± 0.01ns
syringol2.3 ± 0.6 c2.0 ± 0.01 c6.3 ± 0.6 a5.3 ± 0.6 b<0.001
4-methylsyringolndndndnd–
guaiacol glycosides9.8 ± 0.9 c10.3 ± 1.8 c340 ± 22.7 a258 ± 15 b<0.001
4-methylguaiacol glycosides2.9 ± 0.4 c2.3 ± 0.4 c79 ± 2.4 a62 ± 1.2 b<0.001
phenol glycosides3.0 ± 0.2 c3.4 ± 0.7 c111 ± 2.4 a98 ± 2.1 b<0.001
cresol glycosides4.3 ± 0.1 c4.3 ± 0.9 c117 ± 4.9 a100 ± 3.3 b<0.001
syringol glycosides12.2 ± 0.7 c13.4 ± 0.6 c614 ± 5.1 a473 ± 15 b<0.001
4-methylsyringol glycosidestrtr38 ± 1.2 a26 ± 0.6 b<0.001
Data are means from three replicates (n = 3) ± standard deviation; nd = not detected (<0.5 µg/L); tr = trace (0.5–1 µg/L). Different letters (within rows) indicate statistical significance (p = 0.05, one-way ANOVA); ns = not significant. Volatile phenol glycosides measured as syringol glucose–glucoside equivalents.
Table
Table 2. Basic chemistry of Cabernet Sauvignon wines made with control or smoke-exposed grapes, with or without post-harvest ozone treatment (1 ppm for 24 h).
Table 2. Basic chemistry of Cabernet Sauvignon wines made with control or smoke-exposed grapes, with or without post-harvest ozone treatment (1 ppm for 24 h).
ControlControl + O3SmokeSmoke + O3p
pH3.65 ± 0.093.71 ± 0.013.63 ± 0.043.62 ± 0.05ns
TA (g/L)7.3 ± 0.2 a6.9 ± 0.06 b6.9 ± 0.17 b6.9 ± 0.16 b0.011
alcohol (% abv)11.9 ± 0.05 a11.3 ± 0.07 b10.8 ± 0.05 b10.5 ± 0.04 b<0.001
wine color density (au)4.9 ± 0.36 a4.2 ± 0.18 b4.1 ± 0.09 b4.0 ± 0.06 b0.002
wine color hue0.79 ± 0.01 b0.85 ± 0.01 a0.87 ± 0.01 a0.85 ± 0.01 a<0.001
Data are means from three replicates (n = 3) ± standard deviation. Different letters (within rows) indicate statistical significance (p = 0.05, one-way ANOVA).
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Modesti, M.; Szeto, C.; Ristic, R.; Jiang, W.; Culbert, J.; Catelli, C.; Mencarelli, F.; Tonutti, P.; Wilkinson, K. Amelioration of Smoke Taint in Cabernet Sauvignon Wine via Post-Harvest Ozonation of Grapes. Beverages 2021, 7, 44. https://0-doi-org.brum.beds.ac.uk/10.3390/beverages7030044

AMA Style

Modesti M, Szeto C, Ristic R, Jiang W, Culbert J, Catelli C, Mencarelli F, Tonutti P, Wilkinson K. Amelioration of Smoke Taint in Cabernet Sauvignon Wine via Post-Harvest Ozonation of Grapes. Beverages. 2021; 7(3):44. https://0-doi-org.brum.beds.ac.uk/10.3390/beverages7030044

Chicago/Turabian Style

Modesti, Margherita, Colleen Szeto, Renata Ristic, WenWen Jiang, Julie Culbert, Cesare Catelli, Fabio Mencarelli, Pietro Tonutti, and Kerry Wilkinson. 2021. "Amelioration of Smoke Taint in Cabernet Sauvignon Wine via Post-Harvest Ozonation of Grapes" Beverages 7, no. 3: 44. https://0-doi-org.brum.beds.ac.uk/10.3390/beverages7030044

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