Next Article in Journal
Multilocus Genotyping of Giardia duodenalis Occurring in Korean Native Calves
Previous Article in Journal
Prevalence and Temporal Dynamics of White Line Disease in Sheep: An Exploratory Investigation into Disease Distribution and Associated Risk Factors
Previous Article in Special Issue
In Vitro Activity of Several Essential Oils Extracted from Aromatic Plants against Ascosphaera apis
Article

Replicative Deformed Wing Virus Found in the Head of Adults from Symptomatic Commercial Bumblebee (Bombus terrestris) Colonies

CREA Research Centre for Agriculture and Environment, Via di Saliceto 80, 40128 Bologna, Italy
*
Author to whom correspondence should be addressed.
Academic Editor: Yanping (Judy) Chen
Received: 20 May 2021 / Revised: 16 June 2021 / Accepted: 21 June 2021 / Published: 23 June 2021
(This article belongs to the Special Issue Challenges and Advances in Bee Health and Diseases)
The deformed wing virus (DWV) is one of the most common honey bee pathogens. The virus may also be detected in other insect species, including Bombus terrestris adults from wild and managed colonies. In this study, individuals of all stages, castes, and sexes were sampled from three commercial colonies exhibiting the presence of deformed workers and analysed for the presence of DWV. Adults (deformed individuals, gynes, workers, males) had their head exscinded from the rest of the body and the two parts were analysed separately by RT-PCR. Juvenile stages (pupae, larvae, and eggs) were analysed undissected. All individuals tested positive for replicative DWV, but deformed adults showed a higher number of copies compared to asymptomatic individuals. Moreover, they showed viral infection in their heads. Sequence analysis indicated that the obtained DWV amplicons belonged to a strain isolated in the United Kingdom. Further studies are needed to characterize the specific DWV target organs in the bumblebees. The result of this study indicates the evidence of DWV infection in B. terrestris specimens that could cause wing deformities, suggesting a relationship between the deformities and the virus localization in the head. Further studies are needed to define if a specific organ could be a target in symptomatic bumblebees. View Full-Text
Keywords: bumblebee; commercial Bombus terrestris; spillover; DWV; honey bee pathogens; replicative virus; strand-specific RT-PCR bumblebee; commercial Bombus terrestris; spillover; DWV; honey bee pathogens; replicative virus; strand-specific RT-PCR
Show Figures

Figure 1

MDPI and ACS Style

Cilia, G.; Zavatta, L.; Ranalli, R.; Nanetti, A.; Bortolotti, L. Replicative Deformed Wing Virus Found in the Head of Adults from Symptomatic Commercial Bumblebee (Bombus terrestris) Colonies. Vet. Sci. 2021, 8, 117. https://0-doi-org.brum.beds.ac.uk/10.3390/vetsci8070117

AMA Style

Cilia G, Zavatta L, Ranalli R, Nanetti A, Bortolotti L. Replicative Deformed Wing Virus Found in the Head of Adults from Symptomatic Commercial Bumblebee (Bombus terrestris) Colonies. Veterinary Sciences. 2021; 8(7):117. https://0-doi-org.brum.beds.ac.uk/10.3390/vetsci8070117

Chicago/Turabian Style

Cilia, Giovanni, Laura Zavatta, Rosa Ranalli, Antonio Nanetti, and Laura Bortolotti. 2021. "Replicative Deformed Wing Virus Found in the Head of Adults from Symptomatic Commercial Bumblebee (Bombus terrestris) Colonies" Veterinary Sciences 8, no. 7: 117. https://0-doi-org.brum.beds.ac.uk/10.3390/vetsci8070117

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop