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Evaluating the Effect of Lignocellulose-Derived Microbial Inhibitors on the Growth and Lactic Acid Production by Bacillus coagulans Azu-10
Article

Efficient Co-Utilization of Biomass-Derived Mixed Sugars for Lactic Acid Production by Bacillus coagulans Azu-10

1
Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11884, Egypt
2
Al-Azhar Center for Fermentation Biotechnology and Applied Microbiology, Al-Azhar University, Nasr City, Cairo 11884, Egypt
3
Agriculture Microbiology Department, Soil, Water and Environment Research Institute (SWERI), Sakha Agricultural Research Station, Agriculture Research Center (ARC), Giza 11651, Egypt
*
Author to whom correspondence should be addressed.
Academic Editor: Plessas Stavros
Fermentation 2021, 7(1), 28; https://doi.org/10.3390/fermentation7010028
Received: 27 January 2021 / Revised: 12 February 2021 / Accepted: 14 February 2021 / Published: 18 February 2021
Lignocellulosic and algal biomass are promising substrates for lactic acid (LA) production. However, lack of xylose utilization and/or sequential utilization of mixed-sugars (carbon catabolite repression, CCR) from biomass hydrolysates by most microorganisms limits achievable titers, yields, and productivities for economical industry-scale production. This study aimed to design lignocellulose-derived substrates for efficient LA production by a thermophilic, xylose-utilizing, and inhibitor-resistant Bacillus coagulans Azu-10. This strain produced 102.2 g/L of LA from 104 g/L xylose at a yield of 1.0 g/g and productivity of 3.18 g/L/h. The CCR effect and LA production were investigated using different mixtures of glucose (G), cellobiose (C), and/or xylose (X). Strain Azu-10 has efficiently co-utilized GX and CX mixture without CCR; however, total substrate concentration (>75 g/L) was the only limiting factor. The strain completely consumed GX and CX mixture and homoferemnatively produced LA up to 76.9 g/L. On the other hand, fermentation with GC mixture exhibited obvious CCR where both glucose concentration (>25 g/L) and total sugar concentration (>50 g/L) were the limiting factors. A maximum LA production of 50.3 g/L was produced from GC mixture with a yield of 0.93 g/g and productivity of 2.09 g/L/h. Batch fermentation of GCX mixture achieved a maximum LA concentration of 62.7 g/L at LA yield of 0.962 g/g and productivity of 1.3 g/L/h. Fermentation of GX and CX mixture was the best biomass for LA production. Fed-batch fermentation with GX mixture achieved LA production of 83.6 g/L at a yield of 0.895 g/g and productivity of 1.39 g/L/h. View Full-Text
Keywords: lactic acid; designed biomass; lignocellulose-derived sugars; mixed sugars; B. coagulans; CCR; homofermentation lactic acid; designed biomass; lignocellulose-derived sugars; mixed sugars; B. coagulans; CCR; homofermentation
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MDPI and ACS Style

Abdel-Rahman, M.A.; Hassan, S.E.-D.; Alrefaey, H.M.A.; Elsakhawy, T. Efficient Co-Utilization of Biomass-Derived Mixed Sugars for Lactic Acid Production by Bacillus coagulans Azu-10. Fermentation 2021, 7, 28. https://0-doi-org.brum.beds.ac.uk/10.3390/fermentation7010028

AMA Style

Abdel-Rahman MA, Hassan SE-D, Alrefaey HMA, Elsakhawy T. Efficient Co-Utilization of Biomass-Derived Mixed Sugars for Lactic Acid Production by Bacillus coagulans Azu-10. Fermentation. 2021; 7(1):28. https://0-doi-org.brum.beds.ac.uk/10.3390/fermentation7010028

Chicago/Turabian Style

Abdel-Rahman, Mohamed A., Saad E.-D. Hassan, Hassan M.A. Alrefaey, and Tamer Elsakhawy. 2021. "Efficient Co-Utilization of Biomass-Derived Mixed Sugars for Lactic Acid Production by Bacillus coagulans Azu-10" Fermentation 7, no. 1: 28. https://0-doi-org.brum.beds.ac.uk/10.3390/fermentation7010028

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