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Article

Influence of Nitrogen Sources on D-Lactic Acid Biosynthesis by Sporolactobacillus laevolacticus DSM 442 Strain

1
Department of Chemical Technology and Biotechnology, Łukasiewicz Research Network-Institute of Industrial Organic Chemistry, Annopol 6, 03-236 Warsaw, Poland
2
Agrochemical Solutions Hortulanus, Sygietyńskiego 25, 96-316 Stare Budyhort, Poland
*
Authors to whom correspondence should be addressed.
Academic Editors: Mohamed Ali Abdel-Rahman and Luca Settanni
Received: 12 April 2021 / Revised: 10 May 2021 / Accepted: 11 May 2021 / Published: 14 May 2021
The purpose of this study was to explore the possibility of replacing an expensive yeast extract contained in the fermentation medium for D-lactic acid (D-LA, R-lactic acid) biosynthesis with an alternative nitrogen source. The screening studies were conducted under stationary conditions and showed that pea seed hydrolysate was the most beneficial substrate in the process of D-LA biosynthesis by the strain Sporolactobacillus laevolacticus DSM 442 among the used inorganic and organic nitrogen sources, waste materials, food and agricultural products. After 96 h, 75.5 g/L D-LA was obtained in batch cultures in a medium containing pea seed hydrolysate, with an average productivity of 0.79 g/L/h, yield of 75.5%, and optical purity of 99.4%. In batch cultures fed once, in a medium with an analogous composition, 122.6 g/L D-LA was obtained after 120 h, and the average yield, productivity and optical purity were 87.6%, 1.021 g/L/h, and 99.6%, respectively. Moreover, the amount of D-LA obtained in the fermentation medium enriched with the above-mentioned cheap agricultural product was similar to the amounts obtained in the medium containing yeast extract in both stationary and bioreactor cultures. Our research shows that hydrolyzed pea seeds, which belong to the legume family, may be a promising nitrogen source for the production of D-LA on an industrial scale. View Full-Text
Keywords: D-lactic acid; biosynthesis; Sporolactobacillus laevolacticus DSM 442; nitrogen source; agricultural products D-lactic acid; biosynthesis; Sporolactobacillus laevolacticus DSM 442; nitrogen source; agricultural products
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MDPI and ACS Style

Michalczyk, A.K.; Garbaczewska, S.; Morytz, B.; Białek, A.; Zakrzewski, J. Influence of Nitrogen Sources on D-Lactic Acid Biosynthesis by Sporolactobacillus laevolacticus DSM 442 Strain. Fermentation 2021, 7, 78. https://0-doi-org.brum.beds.ac.uk/10.3390/fermentation7020078

AMA Style

Michalczyk AK, Garbaczewska S, Morytz B, Białek A, Zakrzewski J. Influence of Nitrogen Sources on D-Lactic Acid Biosynthesis by Sporolactobacillus laevolacticus DSM 442 Strain. Fermentation. 2021; 7(2):78. https://0-doi-org.brum.beds.ac.uk/10.3390/fermentation7020078

Chicago/Turabian Style

Michalczyk, Alicja K., Sylwia Garbaczewska, Bolesław Morytz, Arkadiusz Białek, and Jerzy Zakrzewski. 2021. "Influence of Nitrogen Sources on D-Lactic Acid Biosynthesis by Sporolactobacillus laevolacticus DSM 442 Strain" Fermentation 7, no. 2: 78. https://0-doi-org.brum.beds.ac.uk/10.3390/fermentation7020078

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