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Article

‘TeeBot’: A High Throughput Robotic Fermentation and Sampling System

1
Department of Wine Science, The University of Adelaide, Glen Osmond, SA 5064, Australia
2
Australian Research Council Training Centre for Innovative Wine Production, Adelaide, SA 5064, Australia
*
Author to whom correspondence should be addressed.
Academic Editors: Claudia Gonzalez Viejo and Sigfredo Fuentes
Received: 15 August 2021 / Revised: 18 September 2021 / Accepted: 18 September 2021 / Published: 24 September 2021
(This article belongs to the Special Issue Implementation of Digital Technologies on Beverage Fermentation)
When fermentation research requires the comparison of many strains or conditions, the major bottleneck is a technical one. Microplate approaches are not able to produce representative fermentative performance due to their inability to truly operate anaerobically, whilst more traditional methods do not facilitate sample density sufficient to assess enough candidates to be considered even medium throughput. Two robotic platforms have been developed that address these technological shortfalls. Both are built on commercially available liquid handling platforms fitted with custom labware. Results are presented detailing fermentation performance as compared to current best practice, i.e., shake flasks fitted with airlocks and sideports. The ‘TeeBot’ is capable sampling from 96 or 384 fermentations in 100 mL or 30 mL volumes, respectively, with airlock sealing and minimal headspace. Sampling and downstream analysis are facilitated by automated liquid handling, use of 96-well sample plate format and temporary cryo-storage (<0 °C). View Full-Text
Keywords: TeeBot; fermentation; high throughput; liquid handling robot; metabolite analysis TeeBot; fermentation; high throughput; liquid handling robot; metabolite analysis
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MDPI and ACS Style

van Holst Pellekaan, N.; Walker, M.E.; Watson, T.L.; Jiranek, V. ‘TeeBot’: A High Throughput Robotic Fermentation and Sampling System. Fermentation 2021, 7, 205. https://0-doi-org.brum.beds.ac.uk/10.3390/fermentation7040205

AMA Style

van Holst Pellekaan N, Walker ME, Watson TL, Jiranek V. ‘TeeBot’: A High Throughput Robotic Fermentation and Sampling System. Fermentation. 2021; 7(4):205. https://0-doi-org.brum.beds.ac.uk/10.3390/fermentation7040205

Chicago/Turabian Style

van Holst Pellekaan, Nicholas, Michelle E. Walker, Tommaso L. Watson, and Vladimir Jiranek. 2021. "‘TeeBot’: A High Throughput Robotic Fermentation and Sampling System" Fermentation 7, no. 4: 205. https://0-doi-org.brum.beds.ac.uk/10.3390/fermentation7040205

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