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Communication

NeuO for Neuronal Labeling in Zebrafish

1
Singapore Bioimaging Consortium, Agency for Science, Technology and Research, Singapore, Singapore
2
Graduate School for Integrative Sciences and Engineering, Centre for LifeSciences, National University of Singapore, Singapore, Singapore
3
Department of Chemistry and MedChem Program of Life Sciences Institute, National University of Singapore, Singapore, Singapore
*
Author to whom correspondence should be addressed.
Submission received: 3 June 2015 / Revised: 8 July 2015 / Accepted: 4 August 2015 / Published: 1 September 2015

Abstract

We report the application of our newly developed fluorescent probe 3-(benzylamino)-4,4-difluoro-5-(4-propyl-1H-1,2,3-triazol-1-yl)-8-(4-(2-hydroxyacetamido)phen-yl)-4-bora-3a,4a-diaza-s-indacene (NeuO) to label and image live neurons in zebrafish. Immersing zebrafish embryos in NeuO or injecting NeuO into zebrafish brain ventricles results in nontoxic in vivo neuronal labeling. We demonstrate the applicability of NeuO and envisage the potential of this compound as a rapid and simple labeling reagent for studying neuron development and degeneration.
Keywords: BODIPY; fluorescent probes; imaging agents; live neurons; zebrafish BODIPY; fluorescent probes; imaging agents; live neurons; zebrafish

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MDPI and ACS Style

Teoh, C.L.; Er, J.C.; Mukherjee, P.; Chang, Y.-T. NeuO for Neuronal Labeling in Zebrafish. Tomography 2015, 1, 30-36. https://0-doi-org.brum.beds.ac.uk/10.18383/j.tom.2015.00127

AMA Style

Teoh CL, Er JC, Mukherjee P, Chang Y-T. NeuO for Neuronal Labeling in Zebrafish. Tomography. 2015; 1(1):30-36. https://0-doi-org.brum.beds.ac.uk/10.18383/j.tom.2015.00127

Chicago/Turabian Style

Teoh, Chai Lean, Jun Cheng Er, Parag Mukherjee, and Young-Tae Chang. 2015. "NeuO for Neuronal Labeling in Zebrafish" Tomography 1, no. 1: 30-36. https://0-doi-org.brum.beds.ac.uk/10.18383/j.tom.2015.00127

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