Next Article in Journal
Spatial Modeling of Potential Lobster Harvest Grounds in Palabuhanratu Bay, West Java, Indonesia
Previous Article in Journal
Effects of Wastewater Treatment Plant Effluent in a Receiving Stream on Reproductive Behavior of Fathead Minnows (Pimephales promelas)
Article

Wound-Induced Changes in Antioxidant Enzyme Activities in Skin Mucus and in Gene Expression in the Skin of Gilthead Seabream (Sparus aurata L.)

Immunobiology for Aquaculture Group, Department of Cell Biology and Histology, Faculty of Biology, University of Murcia, 30100 Murcia, Spain
*
Author to whom correspondence should be addressed.
Academic Editor: Eric Hallerman
Received: 22 March 2021 / Revised: 13 April 2021 / Accepted: 15 April 2021 / Published: 18 April 2021
(This article belongs to the Special Issue New Insights on Fish Immunology)
This study investigated the antioxidant enzyme activities in the skin mucus of gilthead seabream (Sparus aurata L.) at 3 and 7 days post-wounding (dpw). The expression levels of the genes that encode stress proteins (grp170, grp94, grp75, sod and hsp70) and skin regeneration-related proteins (tf, igf1, tgfb1, der1, apo1 and erdj3) in the skin also were determined. Mucus and skin samples were obtained from the left and right flanks of non-wounded and wounded fish. In both flanks of the wounded fish, catalase and glutathione reductase activities in the skin mucus increased (p < 0.05) at 3 and 7 dpw (100 ± 31% and 111 ± 25%, respectively), whereas superoxide dismutase activity increased (p < 0.05) only at 7 dpw (135 ± 15%). The expression levels of stress proteins in the skin of the wounded flank of the wounded fish mainly increased at 7 dpw (grp170 increased to 288 ± 85%, grp94 to 502 ± 143%, grp75 to 274 ± 69%, sod to 569 ± 99%, and hsp70 increased to 537 ± 14%) (p < 0.05). However, the expression levels of the tissue regeneration-related genes varied depending on the flank investigated, on the experimental time, and on the gene studied. To the best of our knowledge, this is the first work to determine the effect of a wound in different skin parts of the same fish. View Full-Text
Keywords: antioxidant enzymes; fish; gilthead seabream (Sparus aurata L.); mucosal immunity; skin healing; skin regeneration antioxidant enzymes; fish; gilthead seabream (Sparus aurata L.); mucosal immunity; skin healing; skin regeneration
Show Figures

Figure 1

MDPI and ACS Style

Espinosa-Ruíz, C.; Esteban, M.Á. Wound-Induced Changes in Antioxidant Enzyme Activities in Skin Mucus and in Gene Expression in the Skin of Gilthead Seabream (Sparus aurata L.). Fishes 2021, 6, 15. https://0-doi-org.brum.beds.ac.uk/10.3390/fishes6020015

AMA Style

Espinosa-Ruíz C, Esteban MÁ. Wound-Induced Changes in Antioxidant Enzyme Activities in Skin Mucus and in Gene Expression in the Skin of Gilthead Seabream (Sparus aurata L.). Fishes. 2021; 6(2):15. https://0-doi-org.brum.beds.ac.uk/10.3390/fishes6020015

Chicago/Turabian Style

Espinosa-Ruíz, Cristóbal, and María Á. Esteban 2021. "Wound-Induced Changes in Antioxidant Enzyme Activities in Skin Mucus and in Gene Expression in the Skin of Gilthead Seabream (Sparus aurata L.)" Fishes 6, no. 2: 15. https://0-doi-org.brum.beds.ac.uk/10.3390/fishes6020015

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop