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Article

Molecular Characterization of Hsp47 in Grass Carp (Ctenopharyngodon idella) and Its Correlation with Type I Collagen in Response to Fish Aerobic Exercise

by 1,2,†, 1,†, 1, 1, 1, 1, 1 and 1,*
1
College of Fisheries, National Demonstration Center for Experimental Aquaculture Education, Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Hubei Provincial Engineering Laboratory for Pond Aquaculture, Huazhong Agricultural University, Wuhan 430070, China
2
Institute of Hydrobiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Maria Angeles Esteban
Received: 22 March 2021 / Revised: 18 April 2021 / Accepted: 19 April 2021 / Published: 23 April 2021
Heat shock protein 47 (Hsp47) is a collagen-specific molecular chaperone that is indispensable for molecular maturation of collagen. In this study, hsp47 and hsp47-like cDNAs were cloned and characterized in grass carp (Ctenopharyngodon idella). The cDNAs were 1212 and 1218 base pairs long, respectively, and included an open reading frame encoding 403 and 405 amino acids. The molecular phylogeny based on the deduced amino acid sequences indicated that the correct sequences of the hsp47 and hsp47-like cDNA were obtained and the deduced proteins clustered distinctly into teleost clades. Primary structure analysis and characterization of Hsp47 and Hsp47-like shared the basic structure and biofunctions of Hsp47 in vertebrates. The spatial pattern of gene expression revealed that hsp47 and hsp47-like were relatively ubiquitous in different tissues and highly expressed in heart and skin. The expression levels of hsp47 and hsp47-like and type I collagen mRNAs varied similarly in different tissues. Type I collagen content increased significantly with the increase of water velocity in the muscle of grass carp in response to aerobic exercise. Among the gene expression changes of hsp47, hsp47-like, col1a1 and col1a2 in muscle that occurred in response to aerobic exercise, the change of type I collagen was most strongly correlated with hsp47 expression. Additionally, col1a1 showed the highest correlation with hsp47-like and col1a2 showed the highest correlation with hsp47. These findings suggest that grass carp Hsp47 and Hsp47-like are closely related to type I collagen synthesis. This study firstly suggests fish aerobic exercise can improve type I collagen content and Hsp47 gene expression in muscle of grass carp. View Full-Text
Keywords: grass carp; hsp47; collagen; molecular cloning; gene expression grass carp; hsp47; collagen; molecular cloning; gene expression
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MDPI and ACS Style

Liang, X.; Wan, Y.; Shen, Z.; Liu, Y.; Li, D.; Li, L.; Tang, R.; Zhang, X. Molecular Characterization of Hsp47 in Grass Carp (Ctenopharyngodon idella) and Its Correlation with Type I Collagen in Response to Fish Aerobic Exercise. Fishes 2021, 6, 17. https://0-doi-org.brum.beds.ac.uk/10.3390/fishes6020017

AMA Style

Liang X, Wan Y, Shen Z, Liu Y, Li D, Li L, Tang R, Zhang X. Molecular Characterization of Hsp47 in Grass Carp (Ctenopharyngodon idella) and Its Correlation with Type I Collagen in Response to Fish Aerobic Exercise. Fishes. 2021; 6(2):17. https://0-doi-org.brum.beds.ac.uk/10.3390/fishes6020017

Chicago/Turabian Style

Liang, Xiao, Ying Wan, Zhiyuan Shen, Yanmei Liu, Dapeng Li, Li Li, Rong Tang, and Xi Zhang. 2021. "Molecular Characterization of Hsp47 in Grass Carp (Ctenopharyngodon idella) and Its Correlation with Type I Collagen in Response to Fish Aerobic Exercise" Fishes 6, no. 2: 17. https://0-doi-org.brum.beds.ac.uk/10.3390/fishes6020017

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