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Article

SOX2OT Long Noncoding RNA Is Regulated by the UPR in Oestrogen Receptor-Positive Breast Cancer

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Auckland Cancer Society Research Centre, University of Auckland, 85 Park Rd, Grafton, Auckland 1023, New Zealand
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Univ Lyon, ISPB Faculty of Pharmacy, INSERM 1052, CNRS5286, Cancer Research Centre of Lyon, F-69008 Lyon, France
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Hospices Civils de Lyon, Biopathology of Tumours, GHE Hospital, F-69500 Bron, France
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Molecular Medicine and Pathology Department, University of Auckland, 85 Park Rd, Grafton, Auckland 1023, New Zealand
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Biochemistry Department, University of Otago, 710 Cumberland Street, Dunedin North, Dunedin 9016, New Zealand
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Murdoch Children’s Research Institute, The Royal Children’s Hospital, Flemington Rd, Parkville, VIC 3052, Australia
*
Authors to whom correspondence should be addressed.
Academic Editors: Aamir Ahmad and Tomoharu Sugie
Received: 6 March 2020 / Revised: 19 March 2020 / Accepted: 20 March 2020 / Published: 26 May 2021
Endoplasmic reticulum (ENR) stress perturbs cell homeostasis and induces the unfolded protein response (UPR). In breast cancer, this process is activated by oestrogen deprivation and is associated with tamoxifen resistance. We present evidence that the transcription factor SOX2 and the long noncoding RNA SOX2 overlapping transcript (SOX2OT) are upregulated in oestrogen receptor-positive (ER+) breast cancer and in response to oestrogen deprivation. We examined the effect of the UPR on SOX2 and SOX2OT expression and the effect of SOX2OT on UPR pathways in breast cancer cell lines. The induction of the UPR by thapsigargin or glucose deprivation upregulates SOX2OT expression. This upregulation is also shown with the anti-oestrogen 4OH-tamoxifen and mTOR inhibitor everolimus in ER + breast cancer cells that are sensitive to oestrogen deprivation or everolimus treatment. SOX2OT overexpression decreased BiP and PERK expression. This effect of SOX2OT overexpression was confirmed on BiP and PERK pathway by q-PCR. Our results show that a long noncoding RNA regulates the UPR and evince a new function of SOX2OT as a participant of ENR stress reprogramming of breast cancer cells. View Full-Text
Keywords: breast cancer; LncRNA; SOX2OT; SOX2; UPR; 4-OH tamoxifen breast cancer; LncRNA; SOX2OT; SOX2; UPR; 4-OH tamoxifen
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MDPI and ACS Style

Ferraro-Peyret, C.; Askarian-Amiri, M.E.; Sarkar, D.; Joseph, W.R.; Hansji, H.; Baguley, B.C.; Leung, E.Y. SOX2OT Long Noncoding RNA Is Regulated by the UPR in Oestrogen Receptor-Positive Breast Cancer. Sci 2021, 3, 26. https://0-doi-org.brum.beds.ac.uk/10.3390/sci3020026

AMA Style

Ferraro-Peyret C, Askarian-Amiri ME, Sarkar D, Joseph WR, Hansji H, Baguley BC, Leung EY. SOX2OT Long Noncoding RNA Is Regulated by the UPR in Oestrogen Receptor-Positive Breast Cancer. Sci. 2021; 3(2):26. https://0-doi-org.brum.beds.ac.uk/10.3390/sci3020026

Chicago/Turabian Style

Ferraro-Peyret, Carole, Marjan E. Askarian-Amiri, Debina Sarkar, Wayne R. Joseph, Herah Hansji, Bruce C. Baguley, and Euphemia Y. Leung 2021. "SOX2OT Long Noncoding RNA Is Regulated by the UPR in Oestrogen Receptor-Positive Breast Cancer" Sci 3, no. 2: 26. https://0-doi-org.brum.beds.ac.uk/10.3390/sci3020026

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