Next Article in Journal
Antioxidants Derived from Natural Products Reduce Radiative Damage in Cultured Retinal Glia to Prevent Oxidative Stress
Previous Article in Journal
Establishment of a Simple and Versatile Evaporation Compensation Model for In Vitro Chronic Ethanol Treatment: Impact on Neuronal Viability
 
 
Article

Fabp7 Is Required for Normal Sleep Suppression and Anxiety-Associated Phenotype following Single-Prolonged Stress in Mice

1
Elson S. Floyd College of Medicine, Washington State University, Spokane, WA 99202, USA
2
Department of Organ Anatomy, Graduate School of Medicine, Tohoku University, Seiryo-cho 2-1, Aobaku, Sendai 980-8575, Japan
3
Steve Gleason Institute for Neuroscience, Washington State University, Spokane, WA 99202, USA
*
Authors to whom correspondence should be addressed.
Academic Editor: James St John
Received: 29 March 2022 / Revised: 14 April 2022 / Accepted: 10 May 2022 / Published: 13 May 2022
Humans with post-traumatic stress disorder (PTSD) exhibit sleep disturbances that include insomnia, nightmares, and enhanced daytime sleepiness. Sleep disturbances are considered a hallmark feature of PTSD; however, little is known about the cellular and molecular mechanisms regulating trauma-induced sleep disorders. Using a rodent model of PTSD called “Single Prolonged Stress” (SPS) we examined the requirement of the brain-type fatty acid binding protein Fabp7, an astrocyte expressed lipid-signaling molecule, in mediating trauma-induced sleep disturbances. We measured baseline sleep/wake parameters and then exposed Fabp7 knock-out (KO) and wild-type (WT) C57BL/6N genetic background control animals to SPS. Sleep and wake measurements were obtained immediately following the initial trauma exposure of SPS, and again 7 days later. We found that active-phase (dark period) wakefulness was similar in KO and WT at baseline and immediately following SPS; however, it was significantly increased after 7 days. These effects were opposite in the inactive-phase (light period), where KOs exhibited increased wake in baseline and following SPS, but returned to WT levels after 7 days. To examine the effects of Fabp7 on unconditioned anxiety following trauma, we exposed KO and WT mice to the light–dark box test before and after SPS. Prior to SPS, KO and WT mice spent similar amounts of time in the lit compartment. Following SPS, KO mice spent significantly more time in the lit compartment compared to WT mice. These results demonstrate that mutations in an astrocyte-expressed gene (Fabp7) influence changes in stress-dependent sleep disturbances and associated anxiety behavior. View Full-Text
Keywords: fear; stress; lipid signaling; blbp; glia; anxiolytic fear; stress; lipid signaling; blbp; glia; anxiolytic
Show Figures

Figure 1

MDPI and ACS Style

Vanderheyden, W.M.; Lefton, M.; Flores, C.C.; Owada, Y.; Gerstner, J.R. Fabp7 Is Required for Normal Sleep Suppression and Anxiety-Associated Phenotype following Single-Prolonged Stress in Mice. Neuroglia 2022, 3, 73-83. https://0-doi-org.brum.beds.ac.uk/10.3390/neuroglia3020005

AMA Style

Vanderheyden WM, Lefton M, Flores CC, Owada Y, Gerstner JR. Fabp7 Is Required for Normal Sleep Suppression and Anxiety-Associated Phenotype following Single-Prolonged Stress in Mice. Neuroglia. 2022; 3(2):73-83. https://0-doi-org.brum.beds.ac.uk/10.3390/neuroglia3020005

Chicago/Turabian Style

Vanderheyden, William M., Micah Lefton, Carlos C. Flores, Yuji Owada, and Jason R. Gerstner. 2022. "Fabp7 Is Required for Normal Sleep Suppression and Anxiety-Associated Phenotype following Single-Prolonged Stress in Mice" Neuroglia 3, no. 2: 73-83. https://0-doi-org.brum.beds.ac.uk/10.3390/neuroglia3020005

Find Other Styles

Article Access Map by Country/Region

1
Back to TopTop