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A Survey of Methodologies for Assessing Mast Cell Density and Activation in Patients with Functional Abdominal Pain Disorders

1
School of Medicine, University of Kansas, Kansas City, KS 66160, USA
2
Division of Gastroenterology, Hepatology, & Nutrition, Children’s Mercy Kansas City, 2401 Gillham Road, Kansas City, MO 64108, USA
3
Department of Pathology, The University of Texas Southwestern Medical Center, 1935 Medical District Drive, Dallas, TX 75235, USA
4
Department of Pediatrics, School of Medicine, University of Missouri-Kansas City, Kansas City, MO 64108, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Takuji Tanaka
Gastrointest. Disord. 2021, 3(4), 142-155; https://0-doi-org.brum.beds.ac.uk/10.3390/gidisord3040016
Received: 23 July 2021 / Revised: 22 September 2021 / Accepted: 28 September 2021 / Published: 30 September 2021
The aim was to assess methods utilized in assessing mast cell involvement in functional abdominal pain disorders (FAPDs), specifically to describe variability in methods utilized to assess both mast cell density and activation and determine if a consensus exists. After a literature search identified 70 manuscripts assessing mast cell density, data were extracted including FAPD diagnosis, site of biopsy, selection of microscopic fields analyzed, selection of mucosal region analyzed, method of mast cell identification, method to assess mast cell density, and if performed, method to assess mast cell activation. There appears to be some consensus favoring inmmunohistochemical stains over histochemical stains for identifying mast cells. Otherwise, considerable variability exists in methodology for assessing mast cell density and activation. Regardless of method, approximately 80% of studies found increased mast cell density and/or activation in comparison to controls with no method being superior. A wide variety of methods have been employed to assess mast cell density and activation with no well-established consensus and inadequate data to recommend specific approaches. The current methodology providing physiologic information needs to be translated to a standard methodology providing clinical information with the development of criteria establishing abnormal density and/or activation, and more importantly, predicting treatment response. View Full-Text
Keywords: mast cells; irritable bowel syndrome; functional dyspepsia mast cells; irritable bowel syndrome; functional dyspepsia
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MDPI and ACS Style

Friesen, H.; Singh, M.; Singh, V.; Schurman, J.V.; Friesen, C.A. A Survey of Methodologies for Assessing Mast Cell Density and Activation in Patients with Functional Abdominal Pain Disorders. Gastrointest. Disord. 2021, 3, 142-155. https://0-doi-org.brum.beds.ac.uk/10.3390/gidisord3040016

AMA Style

Friesen H, Singh M, Singh V, Schurman JV, Friesen CA. A Survey of Methodologies for Assessing Mast Cell Density and Activation in Patients with Functional Abdominal Pain Disorders. Gastrointestinal Disorders. 2021; 3(4):142-155. https://0-doi-org.brum.beds.ac.uk/10.3390/gidisord3040016

Chicago/Turabian Style

Friesen, Hunter, Meenal Singh, Vivekanand Singh, Jennifer V. Schurman, and Craig A. Friesen 2021. "A Survey of Methodologies for Assessing Mast Cell Density and Activation in Patients with Functional Abdominal Pain Disorders" Gastrointestinal Disorders 3, no. 4: 142-155. https://0-doi-org.brum.beds.ac.uk/10.3390/gidisord3040016

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