The Sequential Behavior Pattern Analysis of Broiler Chickens Exposed to Heat Stress
Abstract
:1. Introduction
2. Materials and Methods
2.1. Housing, Animals, and Management
2.2. Experimental Set-Up
2.3. Data Analysis
3. Results
3.1. The Behavioral Frequency Approach
3.2. The GSP Algorithm’s Approach
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
References
- Nääs, I.D.A.; Romanini, C.E.B.; Salgado, D.D.; Lima, K.A.O.; Vale, M.M.D.; Labigalini, M.R.; de Souza, S.R.L.; Menezes, A.G.; de Moura, D.J. Impact of global warming on beef cattle production cost in Brazil. Sci. Agric. 2010, 67, 1–8. [Google Scholar] [CrossRef]
- Vale, M.M.D.; de Moura, D.J.; Nääs, I.A.; Curi, T.M.R.C.; Lima, K.A.O. Effect of a simulated heat wave in thermal and aerial environment broiler-rearing environment. Eng. Agríc. 2016, 36, 271–280. [Google Scholar] [CrossRef] [Green Version]
- Lay, D.C., Jr.; Fulton, R.M.; Hester, P.Y.; Karcher, D.M.; Kjaer, J.B.; Mench, J.A.; Mullens, B.A.; Newberry, R.C.; Nicol, C.J.; O’Sullivan, N.P.; et al. Hen welfare in different housing systems. Poult. Sci. 2011, 90, 278–294. [Google Scholar] [CrossRef]
- Lima, K.A.O.; Nääs, I.A.; Moura, D.J.; Garcia, R.G.; Mendes, A.S. Applying multi-criteria analysis to select the most appropriate broiler rearing environment. Inf. Process. Agric. 2020. [Google Scholar] [CrossRef]
- Daigle, C.L.; Rodenburg, T.B.; Bolhuis, J.E.; Swanson, J.C.; Siegford, J.M. Use of dynamic and rewarding environmental enrichment to alleviate feather pecking in non-cage laying hens. Appl. Anim. Behav. Sci. 2014, 161, 75–85. [Google Scholar] [CrossRef]
- Schiassi, L.; Yangai, T., Jr.; Ferraz, P.F.P.; Campos, A.T.; Silva, G.R.E.; Abreu, L.H.P. Comportamento de frangos de corte submetidos a diferentes ambientes térmicos. Eng. Agríc. 2015, 33, 390–396. [Google Scholar] [CrossRef] [Green Version]
- Mortensen, A.K.; Lisouski, P.; Ahrendt, P. Weight prediction of broiler chickens using 3D computer vision. Comput. Electron. Agric. 2016, 123, 319–326. [Google Scholar] [CrossRef]
- Branco, T.; Moura, D.J.; Nääs, I.A.; Oliveira, S.R.M. Detection of broiler heat stress by using the generalised sequential pattern algorithm. Biosyst. Eng. 2020, 199, 121–126. [Google Scholar] [CrossRef]
- Xin, H.; Shao, J. Real-time assessment of swine thermal comfort by computer vision. In Proceedings of the World Congress of Computers in Agriculture and Natural Resources, Foz do Iguaçu, Brazil, 13–15 March 2002; pp. 362–369. [Google Scholar]
- Cordeiro, M.B.; Tinôco, I.F.F.; Filho, R.M.D.M.; de Sousa, F.C. Análise de imagens digitais para a avaliação do comportamento de pintainhos de corte. Eng. Agríc. 2011, 31, 418–426. [Google Scholar] [CrossRef] [Green Version]
- Van Hertem, T.; Norton, T.; Berckmans, D.; Vranken, E. Predicting broiler gait scores from activity monitoring and flock data. Biosyst. Eng. 2018, 173, 93–102. [Google Scholar] [CrossRef]
- Pereira, D.F.; Miyamoto, B.C.B.; Maia, G.D.N.; Sales, G.T.; Magalhães, M.M.; Gates, R.S. Machine vision to identify broiler breeder behavior. Comput. Electron. Agric. 2013, 99, 194–199. [Google Scholar] [CrossRef]
- Nicol, C.J.; Caplen, G.; Edgar, J.; Browne, W.J. Associations between welfare indicators and environmental choice in laying hens. Anim. Behav. 2009, 78, 413–424. [Google Scholar] [CrossRef]
- Edgar, J.L.; Nicol, C.J.; Pugh, C.A.; Paul, E.S. Surface temperature changes in response to handling in domestic chickens. Physiol. Behav. 2013, 119, 195–200. [Google Scholar] [CrossRef] [PubMed]
- Kristensen, H.H.; Cornou, C. Automatic detection of deviations in activity levels in groups of broiler chickens—A pilot study. Biosyst. Eng. 2011, 109, 369–376. [Google Scholar] [CrossRef]
- Fraess, G.A.; Bench, C.J.; Tierney, K.B. Automated behavioural response assessment to a feeding event in two heritage chicken breeds. Appl. Anim. Behav. Sci. 2016, 179, 74–81. [Google Scholar] [CrossRef]
- Frost, A.R.; Schofield, C.P.; Beaulah, S.A.; Mottram, T.; Lines, J.; Wathes, C.M. A review of livestock monitoring and the need for integrated systems. Comput. Electron. Agric. 1997, 17, 139–159. [Google Scholar] [CrossRef]
- Diez-Olivan, A.; Averós, X.; Sanz, R.; Sierra, B.; Estevez, I. Quantile regression forests-based modeling and environmental indicators for decision support in broiler farming. Comput. Electron. Agric. 2019, 161, 141–150. [Google Scholar] [CrossRef]
- Fournel, S.; Rousseau, A.N.; Laberge, B. Rethinking environment control strategy of confined animal housing systems through precision livestock farming. Biosyst. Eng. 2017, 155, 96–123. [Google Scholar] [CrossRef]
- Morota, G.; Ventura, R.V.; Silva, F.F.; Koyama, M.; Fernando, S.C. Big Data Analytics and Precision Animal Agriculture Symposium: Machine learning and data mining advance predictive big data analysis in precision animal agriculture1. J. Anim. Sci. 2018, 96, 1540–1550. [Google Scholar] [CrossRef]
- Agrawal, R.; Srikant, R. Mining sequential patterns. In Proceedings of the Eleventh International Conference on Data Engineering, Taipei, Taiwan, 6–10 March 1995; pp. 3–14. [Google Scholar] [CrossRef]
- Höpken, W.; Müller, M.; Fuchs, M.; Lexhagen, M. Flickr data for analysing tourists’ spatial behaviour and movement patterns. J. Hosp. Manage. Tour. 2020, 11, 69–82. [Google Scholar] [CrossRef]
- Shih, W.-C. Mining Learners’ Behavioral Sequential Patterns in a Blockly Visual Programming Educational Game. In Proceedings of the 2017 International Conference on Industrial Engineering, Management Science and Application (ICIMSA), Seoul, Korea, 13–15 June 2017; IEEE: Piscataway, NJ, USA, 2017; pp. 1–2. [Google Scholar] [CrossRef]
- Astuti, T.; Anggraini, L. Analysis of Sequential Book Loan Data Pattern Using Generalized Sequential Pattern (GSP) Algorithm. IJIIS Int. J. Inform. Inf. Syst. 2019, 2, 17–23. [Google Scholar] [CrossRef]
- Hubbard. Technical Manual. 2014. Available online: https://www.hubbardbreeders.com/pt/documentation/ (accessed on 27 October 2020).
- Rostagno, S.R.; Albino, L.F.T.; Donzele, J.L.; Gomes, P.C.; Oliveira, R.F.; Lopes, S.C.; Ferreira, A.S.; Barreto, S.L.T.; Euclides, R.F. Tabelas Brasileiras para Aves e Suínos: Composição de Alimentos e Exigências Nutricionais, 3rd ed.; UFV: Viçosa, Brazil, 2011; p. 252. [Google Scholar]
- Weeks, C.A.; Danbury, T.C.; Davies, H.C.; Hunt, P.; Kestin, S.C. The behaviour of broiler chickens and its modification by lameness. Appl. Anim. Behav. Sci. 2000, 67, 111–125. [Google Scholar] [CrossRef]
- Bokkers, E.A.M.; Koene, P. Behaviour of fast- and slow growing broilers to 12 weeks of age and the physical consequences. Appl. Anim. Behav. Sci. 2003, 81, 59–72. [Google Scholar] [CrossRef]
- Nääs, I.D.A.; Lozano, L.C.M.; Mehdizadeh, S.A.; Garcia, R.G.; Abe, J.M. Paraconsistent logic used for estimating the gait score of broiler chickens. Biosyst. Eng. 2018, 173, 115–123. [Google Scholar] [CrossRef]
- Bizeray, D.; Estevez, I.; Leterrier, C.; Faure, J.M. Influence of increased environmental complexity on leg condition, performance, and level of fearfulness in broilers. Poult. Sci. 2002, 81, 767–773. [Google Scholar] [CrossRef]
- Roll, V.F.B.; dai Prá, M.A.; Roll, A.A.P.; Xavier, E.G.; Rossi, P.; Anciuti, M.A.; Rutz, F. Influência da altura de comedouros tubulares no comportamento ingestivo de frangos de corte. Arch. Zootec. 2010, 9, 115–122. Available online: http://scielo.isciii.es/scielo.php?script=sci_arttext&pid=S0004-05922010000100012&lng=es&nrm=iso (accessed on 27 April 2020). (In Portuguese). [CrossRef] [Green Version]
- Srikant, R.; Agrawal, R. Mining sequential patterns: Generalizations and performance improvements. In Transactions on Petri Nets and Other Models of Concurrency XV; Springer Science and Business Media LLC: Berlin/Heidelberg, Germany, 1996; pp. 1–17. [Google Scholar]
- Bureva, V.; Sotirova, E.; Chountas, P. Generalized Net of the Process of Sequential Pattern Mining by Generalized Sequential Pattern Algorithm (GSP). In Advances in Intelligent Systems and Computing; Springer Science and Business Media LLC: Berlin/Heidelberg, Germany, 2015; pp. 831–838. [Google Scholar]
- Witten, I.H.; Frank, E.; Hall, M.A.; Pal, C.J. Data Mining: Practical Machine Learning Tools and Techniques, 4th ed.; Morgan Kaufmann: San Francisco, CA, USA, 2016. [Google Scholar]
- Youssef, A.; Exadaktylos, V.; Berckmans, D.A. Towards real-time control of chicken activity in a ventilated chamber. Biosyst. Eng. 2015, 135, 31–43. [Google Scholar] [CrossRef]
- Sassi, N.B.; Averós, X.; Estevez, I. Technology and Poultry Welfare. Animals 2016, 6, 62. [Google Scholar] [CrossRef] [Green Version]
- Fernández, A.P.; Norton, T.; Tullo, E.; van Hertem, T.; Youssef, A.; Exadaktylos, V.; Vranken, E.; Guarino, M.; Berckmans, D. Real-time monitoring of broiler flock’s welfare status using camera-based technology. Biosyst. Eng. 2018, 173, 103–114. [Google Scholar] [CrossRef]
- Li, G.; Hui, X.; Lin, F.; Zhao, Y. Developing and Evaluating Poultry Preening Behavior Detectors via Mask Region-Based Convolutional Neural Network. Animals 2020, 10, 1762. [Google Scholar] [CrossRef]
- Kashiha, M.; Pluk, A.; Bahr, C.; Vranken, E.; Berckmans, D. Development of an early warning system for a broiler house using computer vision. Biosyst. Eng. 2013, 116, 36–45. [Google Scholar] [CrossRef]
- Guo, Y.; Chai, L.; Aggrey, S.E.; Oladeinde, A.; Johnson, J.; Zock, G. A Machine Vision-Based Method for Monitoring Broiler Chicken Floor Distribution. Sensors 2020, 20, 3179. [Google Scholar] [CrossRef]
- Chowdhury, V.S.; Tomonaga, S.; Nishimura, S.; Tabata, S.; Furuse, M. Physiological and Behavioral Responses of Young Chicks to High Ambient Temperature. J. Poult. Sci. 2012, 49, 212–218. [Google Scholar] [CrossRef] [Green Version]
- Li, G.; Zhao, Y.; Chesser, G.D.; Lowe, J.W.; Purswell, J.L. Image Processing for Analyzing Broiler Feeding and Drinking Behaviors. In Proceedings of the 2019 ASABE Annual International Meeting, Boston, MA, USA, 7–10 July 2019; American Society of Agricultural and Biological Engineers (ASABE): Boston, MA, USA, 2019. [Google Scholar]
- Santos, M.M.; Souza-Junior, J.B.F.; Queiroz, J.P.A.F.; Costa, M.K.O.; Lima, H.F.F.; Arruda, A.M.V.; Costa, L.L.M. Broilers’ behavioural adjustments when submitted to natural heat stress and fed different maize particle sizes in the diet. J. Agric. Sci. 2019, 157, 743–748. [Google Scholar] [CrossRef]
- Vandana, G.D.; Sejian, V.; Lees, A.M.; Pragna, P.; Silpa, M.V.; Maloney, S.K. Heat stress and poultry production: Impact and amelioration. Int. J. Biometeorol. 2021, 65, 163–179. [Google Scholar] [CrossRef]
- María, G.A.; Escós, J.; Alados, C.L. Complexity of behavioural sequences and their relation to stress conditions in chickens (Gallus gallus domesticus): A non-invasive technique to evaluate animal welfare. Appl. Anim. Behav. Sci. 2004, 86, 93–104. [Google Scholar] [CrossRef] [Green Version]
- Mack, L.A.; Felver-Gant, J.N.; Dennis, R.L.; Cheng, H.W. Genetic variations alter production and behavioral responses following heat stress in 2 strains of laying hens. Poult. Sci. 2013, 92, 285–294. [Google Scholar] [CrossRef] [PubMed]
- Rushen, J.; Butterworth, A.; Swanson, J.C. Animal Behavior and Well-Being Symposium: Farm animal welfare assurance: Science and application 1. J. Anim. Sci. 2011, 89, 1219–1228. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Zhao, Z.-G.; Li, J.-H.; Li, X.; Bao, J. Effects of Housing Systems on Behaviour, Performance and Welfare of Fast-growing Broilers. Asian-Australas. J. Anim. Sci. 2014, 27, 140–146. [Google Scholar] [CrossRef] [Green Version]
- Filho, J.A.D.B.; Silva, I.J.O.; Silva, M.A.N.; Silva, C.J.M. Avaliação dos comportamentos de aves poedeiras utilizando sequência de imagens. Eng. Agríc. 2007, 27, 93–99. [Google Scholar] [CrossRef] [Green Version]
- Henson, S.M.; Weldon, L.M.; Hayward, J.L.; Greene, D.J.; Megna, L.C.; Serem, M.C. Coping behaviour as an adaptation to stress: Post-disturbance preening in colonial seabirds. J. Biol. Dyn. 2012, 6, 17–37. [Google Scholar] [CrossRef] [PubMed]
- Quinteiro-Filho, W.M.; Ribeiro, A.; Ferraz-De-Paula, V.; Pinheiro, M.L.; Sakai, M.; Sá, L.R.M.; Ferreira, A.J.P.; Palermo-Neto, J. Heat stress impairs performance parameters, induces intestinal injury, and decreases macrophage activity in broiler chickens. Poult. Sci. 2010, 89, 1905–1914. [Google Scholar] [CrossRef] [PubMed]
- Dawkins, M.S. Animal welfare and efficient farming: Is conflict inevitable? Anim. Prod. Sci. 2016, 57, 201. [Google Scholar] [CrossRef]
Nutritional Levels | Age (Week) | ||
---|---|---|---|
4th | 5th | 6th | |
Metabolizable energy (kcal/kg) | 3153 | 3198 | 3247 |
Crude protein (%) | 19.87 | 19.03 | 18.16 |
Calcium (%) | 0.75 | 0.66 | 0.61 |
Digestible phosphorus (%) | 0.29 | 0.28 | 0.26 |
Sodium (%) | 0.20 | 0.20 | 0.19 |
Digestible lysine (%) | 1.10 | 1.05 | 1.00 |
Digestible methionine (%) | 0.57 | 0.56 | 0.53 |
Digestible methionine + cystine (%) | 0.80 | 0.77 | 0.73 |
Age (Week) | Thermoneutral Temperature * (°C) | Air Relative Humidity (%) | Heat Stress (°C) | Air Relative Humidity (%) |
---|---|---|---|---|
4th | 20.00 ± 1.3 | 73.35 ± 2.1 | 28.00 ± 1.0 | 61.01 ± 2.5 |
5th | 19.00 ± 0.9 | 69.45 ± 2.5 | 27.00 ± 1.1 | 57.41 ± 2.3 |
6th | 18.00 ± 0.7 | 74.21 ± 2.3 | 26.00 ± 0.9 | 63.61 ± 2.0 |
Ethogram of Observed Behaviors | |
---|---|
Eating | The bird is in front of the feeder and ingests feed |
Drinking | The bird is in front of the drinker and ingests water |
Foraging | The bird stands in an upright position and uses both feet to peck at or move litter material in search of food |
Lying down | The bird lies in the litter while the head is resting on the ground or is erect |
Walking | The bird moves at a slow pace |
Running | The bird moves at a fast pace (at least three steps quicker than normal * walking) |
Preening | The bird cleans and aligns the feathers using the beak |
Litter pecking | The bird pecks the litter with the beak |
Wing flap | Flaps wings while standing on the ground |
Dust bathing | Bathing in the dust with the use of wings, head, neck, and legs |
Stretching | The bird stretches one wing and one leg of the same body hemisphere |
Lying laterally | The bird lies laterally with a stretched leg |
Observed Behavior | ||||||||
---|---|---|---|---|---|---|---|---|
Age (Wk) | Ambient Condition | Lying Down | Eating | Walking | Preening | Lying Laterally | Drinking | Dust Bathing |
4th | HS | 18.17 ± 6.10 a | 9.31 ± 3.22 a | 18.00 ± 8.88 a | 4.65 ± 2.49 a | 1.65 ± 1.51 a | 4.00 ± 2.63 a | 0.167 ± 0.78 a |
TNZ | 17.35 ± 4.69 a | 10.56 ± 3.75 a | 15.63 ± 9.49 a | 2.92 ± 2.14 b | 0.40 ± 0.74 b | 3.90 ± 3.23 a | 0.104 ± 0.31 a | |
p-value | 0.466 | 0.083 | 0.233 | 0.0001 | 0.0001 | 0.863 | 0.607 | |
5th | HS | 18.06 ± 5.66 a | 7.77 ± 3.02 a | 11.54 ± 6.86 a | 3.85 ± 1.87 a | 2.31 ± 1.79 a | 4.08 ± 2.44 a | 0.19 ± 0.70 a |
TNZ | 16.15 ± 4.98 a | 6.63 ± 2.90 a | 11.88 ± 8.06 a | 3.73 ± 2.29 a | 0.38 ± 0.98 b | 2.79 ± 2.29 b | 0.04 ± 0.20 a | |
p-value | 0.082 | 0.061 | 0.828 | 0.770 | 0.0001 | 0.009 | 0.171 | |
6th | HS | 18.86 ± 5.23 a | 7.00 ± 2.82 a | 11.02 ± 6.34 a | 4.33 ± 2.11 a | 1.54 ± 1.25 a | 4.46 ± 2.70 a | 0.21 ± 0.92 a |
TNZ | 18.98 ± 5.61 a | 8.02 ± 2.88 a | 10.71 ± 7.56 a | 3.48 ± 1.81 b | 0.71 ± 0.94 a | 3.50 ± 2.53 a | 0.06 ± 0.32 a | |
p-value | 0.925 | 0.082 | 0.827 | 0.036 | 0.0001 | 0.076 | 0.303 |
The Pattern of Sequential Behaviors | ||
---|---|---|
Age (Week) | Thermoneutral Temperature | Heat Stress |
4th | <{Eating, Lying down, Eating}> (n = 6) <{Lying down, Walking, Drinking, Walking, Lying down }> (n = 5) <{Lying down, Eating }> (n = 7) <{Eating, Walking, Lying down }> (n = 4) <{Eating, Walking, Lying down, Walking, Eating}> (4) <{Lying down, Walking, Eating}> (n = 5) <{Eating, Walking, Lying down, Walking, Eating, Walking, Lying down}> (n = 4) <{Lying down, Preening}> (n = 6) | <{Lying down, Preening, Walking, Eating}> (n = 5) <{Lying down, Preening, Lying laterally}> (n = 4) <{Lying down, Eating }> (n = 4) <{Eating, Lying down }> (n = 4) <{Lying down, Lying laterally}> (n = 5) <{Lying down, Preening}> (n = 5) |
5th | <{Lying down, Eating }> (n = 4) <{Lying down, Preening}> (n = 8) <{Lying down, Eating, Lying down}> (n = 4) <{Lying down, Walking, Drinking}> (n = 6) <{Lying down, Walking, Eating}> (n = 4) <{Eating, Lying down}> (n = 6) <{Eating, Walking, Lying down, Walking, Eating}> (n = 4) | <{Eating, Lying down}> (n = 7) <{Lying down, Eating, Lying down}>(n = 6) <{Lying down, Preening}> (n = 4) <{Lying down, Eating, Walking, Lying down}> (n = 4) |
6th | <{Lying down, Preening}> (n = 9) <{Lying down, Eating, Lying down}> (n = 8) <{Eating, Walking, Lying down}> (n = 4) <{Lying down, Eating}> (n = 5) <{Eating, Lying down}> (n = 7) | <{Lying down, Preening, Lying laterally}> n = (4) <{Lying down, Walking, Eating, Lying down}> (n = 4) <{Lying down, Preening}> (n = 7) <{Lying down, Lying laterally}> (n = 4) <{Lying down, Eating, Lying down}> (n = 5) <{Eating, Lying down }> (n = 7) |
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Branco, T.; Moura, D.J.d.; de Alencar Nääs, I.; da Silva Lima, N.D.; Klein, D.R.; Oliveira, S.R.d.M. The Sequential Behavior Pattern Analysis of Broiler Chickens Exposed to Heat Stress. AgriEngineering 2021, 3, 447-457. https://0-doi-org.brum.beds.ac.uk/10.3390/agriengineering3030030
Branco T, Moura DJd, de Alencar Nääs I, da Silva Lima ND, Klein DR, Oliveira SRdM. The Sequential Behavior Pattern Analysis of Broiler Chickens Exposed to Heat Stress. AgriEngineering. 2021; 3(3):447-457. https://0-doi-org.brum.beds.ac.uk/10.3390/agriengineering3030030
Chicago/Turabian StyleBranco, Tatiane, Daniella Jorge de Moura, Irenilza de Alencar Nääs, Nilsa Duarte da Silva Lima, Daniela Regina Klein, and Stanley Robson de Medeiros Oliveira. 2021. "The Sequential Behavior Pattern Analysis of Broiler Chickens Exposed to Heat Stress" AgriEngineering 3, no. 3: 447-457. https://0-doi-org.brum.beds.ac.uk/10.3390/agriengineering3030030