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NaHS-Hydrogel and Encapsulated Adipose-Derived Stem Cell Evaluation on an Ex Vivo Second-Degree Burn Model

1
Lyon-Est School of Medicine, University Claude Bernard Lyon-1, 69100 Villeurbanne, France
2
Bank of Tissues and Cells, Lyon University Hospital (Hospices Civils de Lyon), 69003 Lyon, France
3
CarMeN Laboratory, INSERM U1060, INRA U1397, INSA de Lyon, 69600 Oullins, France
4
Fundamental, Clinical and Therapeutic Aspects of Skin Barrier Function, EA4169, University of Lyon 1, 69008 Lyon, France
5
Tissue Biology and Therapeutic Engineering Laboratory, UMR 5305, 69007 Lyon, France
*
Authors to whom correspondence should be addressed.
Those authors contributed equally to this paper.
Academic Editor: Naiem Moiemen
Received: 6 January 2021 / Revised: 7 February 2021 / Accepted: 15 February 2021 / Published: 19 February 2021
Second-degree burns result in the loss of the epidermal barrier and could lead to delayed complications during the healing process. Currently, therapeutic options to treat severe burns are limited. Thus, this work aims to evaluate the effect of NaHS, a hydrogen sulfide (H2S) donor, in poloxamer hydrogel in topical application and the potentiating effect of injected encapsulated adipose-derived stem cells (ASCs) compared to monolayer ASCs using our previous second-degree burn model on human skin explants. Indeed, our model allows testing treatments in conditions similar to a clinical application. The observed benefits of NaHS may include an antioxidant role, which might be beneficial in the case of burns. Concerning ASCs, their interest in wound healing is more than well documented. In order to evaluate the efficiency of our treatments, we analyzed the kinetics of wound closure, keratinocyte proliferation, and dermal remodeling. The effect of NaHS led to a delay in re-epithelialization, with a decrease in the number of proliferating cells and a decrease in the synthesis of procollagen III. On the contrary, intradermal injection of ASCs, encapsulated or not, improves wound healing by accelerating re-epithelialization and collagen I synthesis; however, only encapsulated ASCs accelerate keratinocyte migration and increase the rate of procollagen III and collagen III. In conclusion, NaHS treatment did not improve burn healing. However, the injection of ASCs stimulated wound healing, which is encouraging for their therapeutical use in burn treatment. View Full-Text
Keywords: burn; human skin; ex vivo; wound healing; H2S treatment; ASC treatment; encapsulation burn; human skin; ex vivo; wound healing; H2S treatment; ASC treatment; encapsulation
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MDPI and ACS Style

Capin, L.; Gross-Amat, O.; Calteau, M.; Rovere, M.-R.; Salmon, D.; Auxenfans, C. NaHS-Hydrogel and Encapsulated Adipose-Derived Stem Cell Evaluation on an Ex Vivo Second-Degree Burn Model. Eur. Burn J. 2021, 2, 9-30. https://0-doi-org.brum.beds.ac.uk/10.3390/ebj2010002

AMA Style

Capin L, Gross-Amat O, Calteau M, Rovere M-R, Salmon D, Auxenfans C. NaHS-Hydrogel and Encapsulated Adipose-Derived Stem Cell Evaluation on an Ex Vivo Second-Degree Burn Model. European Burn Journal. 2021; 2(1):9-30. https://0-doi-org.brum.beds.ac.uk/10.3390/ebj2010002

Chicago/Turabian Style

Capin, Lucille, Olivia Gross-Amat, Marie Calteau, Marie-Rose Rovere, Damien Salmon, and Céline Auxenfans. 2021. "NaHS-Hydrogel and Encapsulated Adipose-Derived Stem Cell Evaluation on an Ex Vivo Second-Degree Burn Model" European Burn Journal 2, no. 1: 9-30. https://0-doi-org.brum.beds.ac.uk/10.3390/ebj2010002

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