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Evaluation of the Biocompatibility and Osteoconduction of the Carbon Nanotube, Chitosan and Hydroxyapatite Nanocomposite with or without Mesenchymal Stem Cells as a Scaffold for Bone Regeneration in Rats

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Department of Surgery, School of Veterinary Medicine and Animal Science, University of São Paulo (FMVZ-USP), Prof. Orlando Marques Paiva Avenue 87, Butantã, São Paulo 05508-270, SP, Brazil
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Laboratory of Cell Culture and Regenerative Therapy, Department of Internal Medicine, School of Veterinary Medicine and Animal Science, University of São Paulo (FMVZ-USP), Avenida Prof. Orlando Marques Paiva Avenue 87, Butantã, São Paulo 05508-270, SP, Brazil
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Department of Pathology, School of Veterinary Medicine and Animal Science, University of São Paulo (FMVZ-USP), Prof. Orlando Marques Paiva Avenue 87, Butantã, São Paulo 05508-270, SP, Brazil
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Laboratory of Biopolymers and Biomaterials, Department of Chemistry and Molecular Physics, Institute of Chemistry of São Carlos, University of São Paulo (IQSC-USP), Trabalhador São-Carlense Avenue 400, Parque Arnold Schmidt, São Carlos 13566-590, SP, Brazil
*
Author to whom correspondence should be addressed.
Academic Editor: Rossella Bedini
Received: 20 May 2021 / Revised: 13 July 2021 / Accepted: 22 July 2021 / Published: 27 July 2021
Background: Bone substitutes have been developed to assist bone regeneration in orthopedic surgeries. Mesenchymal stem cells can be added to these biomaterials to enhance bone regeneration. This study aimed to evaluate the biocompatibility and osteoconduction of a carbon nanotube, chitosan, and hydroxyapatite nanocomposite (CNCHN) that had either been enriched or not enriched with sheep bone marrow mesenchymal stem cells (BM-MSCs) in rats. Methods: A total of sixty rats were divided into groups, and an implant with or without BM-MSCs was performed subcutaneously in 20 animals (euthanized after 7 and 30 days), comparing them to 10 control animals, and in the calvaria of 20 animals (euthanized after 20 and 60 days), comparing to with 10 control animals. Subcutaneous and calvaria histologies were performed after euthanasia. Results: The subcutaneous tissue showed that CNCHN did not prompt an exacerbated inflammatory response or signs of necrosis. The histomorphological analysis by the calvaria score of the rats showed that the control group had lower scores at 20 and 60 days for bone neoformation, relative to the CNCHN groups, which showed no significant statistical differences, suggesting that the nanocomposite assisted in the regenerative process of defects in the calvaria, but with no repair potentiation when using BM-MSCs. Conclusion: CNCHN has biocompatibility and osteoconductive potential, showing promising results in bone defects. View Full-Text
Keywords: bone tissue engineering; biomaterials; animal fracture; bone repair bone tissue engineering; biomaterials; animal fracture; bone repair
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MDPI and ACS Style

Marcondes, G.M.; Paretsis, N.F.; Fülber, J.; Navas-Suárez, P.E.; Mori, C.M.C.; Plepis, A.M.G.; Martins, V.C.A.; Fantoni, D.T.; Zoppa, A.L.V. Evaluation of the Biocompatibility and Osteoconduction of the Carbon Nanotube, Chitosan and Hydroxyapatite Nanocomposite with or without Mesenchymal Stem Cells as a Scaffold for Bone Regeneration in Rats. Osteology 2021, 1, 118-131. https://0-doi-org.brum.beds.ac.uk/10.3390/osteology1030013

AMA Style

Marcondes GM, Paretsis NF, Fülber J, Navas-Suárez PE, Mori CMC, Plepis AMG, Martins VCA, Fantoni DT, Zoppa ALV. Evaluation of the Biocompatibility and Osteoconduction of the Carbon Nanotube, Chitosan and Hydroxyapatite Nanocomposite with or without Mesenchymal Stem Cells as a Scaffold for Bone Regeneration in Rats. Osteology. 2021; 1(3):118-131. https://0-doi-org.brum.beds.ac.uk/10.3390/osteology1030013

Chicago/Turabian Style

Marcondes, Geissiane M., Nicole F. Paretsis, Joice Fülber, Pedro E. Navas-Suárez, Claudia M.C. Mori, Ana M.G. Plepis, Virginia C.A. Martins, Denise T. Fantoni, and André L.V. Zoppa 2021. "Evaluation of the Biocompatibility and Osteoconduction of the Carbon Nanotube, Chitosan and Hydroxyapatite Nanocomposite with or without Mesenchymal Stem Cells as a Scaffold for Bone Regeneration in Rats" Osteology 1, no. 3: 118-131. https://0-doi-org.brum.beds.ac.uk/10.3390/osteology1030013

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