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Nucleoredoxin Downregulation Reduces β-Catenin Levels and Shifts Hematopoietic Differentiation towards Myeloid Lineage In Vitro

1
Department of Biochemistry and Molecular Biology, University of Salamanca, 37007 Salamanca, Spain
2
Institute for Biomedical Research of Salamanca (IBSAL), 37007 Salamanca, Spain
*
Author to whom correspondence should be addressed.
Academic Editor: Buyong Ma
BioChem 2021, 1(1), 26-35; https://doi.org/10.3390/biochem1010003
Received: 23 February 2021 / Revised: 26 March 2021 / Accepted: 27 March 2021 / Published: 4 April 2021
The importance of dissecting signaling pathways governing cell differentiation is based on their relevance not only for understanding basic biological phenomena but also for better comprehending the underlying mechanisms of pathologic alterations such as cancer. A paradigm of cell differentiation processes is hematopoiesis, where a single stem cell gives rise to multiple, fully differentiated, cell lineages. Nucleoredoxin (Nrx), a member of the thioredoxin family, is an important redox-sensitive modulator of Wnt/β-catenin signaling, a key pathway for the control of hematopoiesis. In this work, the relevance of Nrx for the differentiation of mouse hematopoietic progenitor cells has been analyzed in vitro. Nrx silencing leads to a dramatic reduction in the size of the Lin and LSK progenitor populations. Moreover, there is also a remarkable decrease in CD3+ cells and an enhancement in the percentage of CD11b+Gr1 myeloid cells. This myeloid bias would agree with the inhibition of the Wnt/β-catenin pathway. Interestingly, a reduction in β-catenin at the protein level was observed upon Nrx silencing. Our results strongly support the importance of Nrx for hematopoietic differentiation, which could be mediated by the regulation of the Wnt/β-catenin pathway. View Full-Text
Keywords: nucleoredoxin; β-catenin; hematopoietic differentiation nucleoredoxin; β-catenin; hematopoietic differentiation
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MDPI and ACS Style

Pérez-Fernández, A.; López-Ruano, G.; Prieto-Bermejo, R.; Sánchez-Bernal, C.; Sánchez-Yagüe, J.; Hernández-Hernández, Á. Nucleoredoxin Downregulation Reduces β-Catenin Levels and Shifts Hematopoietic Differentiation towards Myeloid Lineage In Vitro. BioChem 2021, 1, 26-35. https://0-doi-org.brum.beds.ac.uk/10.3390/biochem1010003

AMA Style

Pérez-Fernández A, López-Ruano G, Prieto-Bermejo R, Sánchez-Bernal C, Sánchez-Yagüe J, Hernández-Hernández Á. Nucleoredoxin Downregulation Reduces β-Catenin Levels and Shifts Hematopoietic Differentiation towards Myeloid Lineage In Vitro. BioChem. 2021; 1(1):26-35. https://0-doi-org.brum.beds.ac.uk/10.3390/biochem1010003

Chicago/Turabian Style

Pérez-Fernández, Alejandro, Guillermo López-Ruano, Rodrigo Prieto-Bermejo, Carmen Sánchez-Bernal, Jesús Sánchez-Yagüe, and Ángel Hernández-Hernández. 2021. "Nucleoredoxin Downregulation Reduces β-Catenin Levels and Shifts Hematopoietic Differentiation towards Myeloid Lineage In Vitro" BioChem 1, no. 1: 26-35. https://0-doi-org.brum.beds.ac.uk/10.3390/biochem1010003

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