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Teladorsagia circumcincta 1,6-Bisphosphate Aldolase: Molecular and Biochemical Characterisation, Structure Analysis and Recognition by Immune Hosts

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AgResearch Ltd., Hopkirk Research Institute, Grasslands Research Centre, Tennent Drive, Private Bag 11008, Palmerston North 4442, New Zealand
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School of Veterinary Sciences, Massey University, Private Bag 11222, Palmerston North 4440, New Zealand
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Author to whom correspondence should be addressed.
Received: 9 December 2020 / Revised: 6 January 2021 / Accepted: 12 January 2021 / Published: 30 January 2021
A 1095 bp full length cDNA encoding Teladorsagia circumcincta aldolase (TciALDO-1) was cloned and expressed in Escherichia coli. Recombinant TciALDO-1 was purified, and its kinetic properties determined. The predicted protein consisted of 365 amino acids, and was present as a single band of about 44 kDa on SDS-PAGE. Multiple alignments of the protein sequence of TciALDO-1 with homologues from other helminths showed the greatest similarity (93%) to the aldolases of Haemonchus contortus and Dictyocaulus viviparus, 82–86% similarity to the other nematode sequences, and 68–71% similarity to cestode and trematode enzymes. Substrate binding sites and conserved regions were identified, and were completely conserved in other homologues. At 30 °C, the optimum pH for TciALDO-1 activity was pH 7.5, the Vmax was 432 ± 23 nmol × min−1 × mg−1 protein, and the apparent Km for the substrate fructose 1,6-bisphosphate was 0.24 ± 0.01 µM (mean ± SEM, n = 3). Recombinant TciALDO-1 was recognized by antibodies in both serum and saliva from field-immune sheep in ELISA, however, that was not the case with nematode-naïve sheep. Teladorsagia circumcincta fructose 1,6-bisphosphate aldolase appears to have potential as a vaccine candidate to control this common sheep parasite. View Full-Text
Keywords: aldolase; cloning; expression; kinetic properties; Teladorsagia circumcincta aldolase; cloning; expression; kinetic properties; Teladorsagia circumcincta
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MDPI and ACS Style

Umair, S.; Bouchet, C.; Palevich, N.; Simpson, H. Teladorsagia circumcincta 1,6-Bisphosphate Aldolase: Molecular and Biochemical Characterisation, Structure Analysis and Recognition by Immune Hosts. Parasitologia 2021, 1, 1-11. https://0-doi-org.brum.beds.ac.uk/10.3390/parasitologia1010001

AMA Style

Umair S, Bouchet C, Palevich N, Simpson H. Teladorsagia circumcincta 1,6-Bisphosphate Aldolase: Molecular and Biochemical Characterisation, Structure Analysis and Recognition by Immune Hosts. Parasitologia. 2021; 1(1):1-11. https://0-doi-org.brum.beds.ac.uk/10.3390/parasitologia1010001

Chicago/Turabian Style

Umair, Saleh, Charlotte Bouchet, Nikola Palevich, and Heather Simpson. 2021. "Teladorsagia circumcincta 1,6-Bisphosphate Aldolase: Molecular and Biochemical Characterisation, Structure Analysis and Recognition by Immune Hosts" Parasitologia 1, no. 1: 1-11. https://0-doi-org.brum.beds.ac.uk/10.3390/parasitologia1010001

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