Micro, Volume 2, Issue 2 (June 2022) – 9 articles

Diatoms are single-cell algae encased in a cell wall (named frustule) composed of transparent, biogenic (or opaline) silica with intricate and strikingly regular patterns. Over the past 30 years, these microorganisms have proven to be a valuable replacement for synthetic silica that satisfies numerous pharmaceutical requirements for the realization of drug delivery vectors, biosensing supports and photonic crystals. It is not only the structural features of the diatoms, but also the possibility of chemically modifying the frustule that permits the relatively straightforward transformation of the biosilica into potential devices for biomedical applications. In this short review, we explore the applications of diatoms-derived biosilica in the drug delivery and biosensing fields. Specifically, we consider the use of diatoms for the targeted delivery of anticancer and antibiotic drugs and how the same microalgae are employed in the fabrications of biosensors whose analyte signal response is evaluated via fluorescence and surface-enhanced Raman scattering techniques. We limit our discussion to studies published in the last seven years, with the intention of minimizing possible redundancy with respect to previously published contributions. Full article

The supramolecular aggregation processes occurring on metallic (aluminum and gold) surfaces in aqueous solutions of bovine serum albumin (BSA) during drying were studied using advanced scanning electron microscopy (SEM). The possible mechanism for the formation of amazing intricate fractal structures on metallic surfaces was proposed based on the analysis of SEM images, size distribution diagrams and EDX-scanning element distribution maps. Full article

Microchannels typically have rectangular cross-sections, whereas biological channels, such as blood vessels and airways, have circular cross-sections. The difference in cross-sections causes different fluidic behaviors, resulting in differences between fluidic behavior in microchannels and biological channels. To simulate fluidic behavior in vitro, circular cross-sectional microchannels are required. We developed a circular cross-sectional microchannel with a smooth channel wall, free from cracks and irregularities. In the fabrication process of the circular cross-sectional microchannel, uncured polydimethylsiloxane (PDMS) was inserted into a rectangular cross-sectional microchannel. Then, the PDMS was pushed out by the introduction of compressed air before the curing process. During the introduction of compressed air, we observed the behavior of the uncured PDMS and found the formation and movement of protrusions along the microchannel. After pushing out the uncured PDMS, the residual PDMS was cured, resulting in a circular cross-sectional microchannel. The fabrication method was examined by varying the channel orientation and airflow direction. In the case of the horizontal orientation and airflow in an opposite direction, the circularity of the microchannel was greater than 0.99 at the centimeter scale in all cross-sections along the microchannel. Full article

Novel biohybrid homo-polyelectrolyte-based nanocarriers were formed by the complexation of insulin (INS) with a biocompatible and cationic polyelectrolyte, namely, poly(vinyl benzyl trimethylammonium chloride) (PVBTMAC). According to light-scattering techniques, the hydrophilic PVBTMAC homo-polyelectrolyte forms single chains in aqueous media. The resulting biohybrid PVBTMAC/INS nanocarriers were formed via electrostatic co-assembly. The effects of polyelectrolyte structure and content on the characteristics of the formed PVBTMAC/INS complexes were studied. A significant aggregation tendency of the PVBTMAC/INS complexes was observed, based on the physicochemical results, especially at high protein concentration, corroborating the effective electrostatic interaction of INS with the cationic polyelectrolyte. The physicochemical properties of the formed PVBTMAC/INS nanocarriers depended on the concentration of the stock polymer and INS solutions. A neat PVBTMAC homo-polymer and PVBTMAC/INS nanocarriers demonstrated good serum stability in the presence of fetal bovine serum (FBS) proteins. Fluorescence spectroscopy (FS) studies revealed no INS conformational changes after its complexation with the cationic PVBTMAC polyelectrolyte. The obtained PVBTMAC/INS complexes demonstrated considerable and promising characteristics for potential use as insulin delivery systems. Full article

The aim of this paper is the accurate and efficient analysis of the surface-plasmon-mode resonances of a graphene microdisk stack in the terahertz range. By means of suitable generalized boundary conditions and Fourier series expansion, the problem is formulated in terms of sets of one-dimensional integral equations in the vector Hankel transform domain for the harmonics of the surface current densities. In virtue of the Helmholtz decomposition, the unknowns are replaced by the corresponding surface curl-free and divergence-free contributions. An approximate solution is achieved by means of the Galerkin method. The proper selection of expansion functions reconstructing the physical behavior of the surface current densities leads to a fast-converging Fredholm second-kind matrix equation, whose elements are accurately and efficiently evaluated by means of a suitable analytical procedure in the complex plane. It is shown that the surface-plasmon-mode resonance frequencies upshift by increasing the number of disks and by decreasing the distance between the disks, and that new resonances can arise for small with respect to the radius distances between the disks, resembling the dipole-mode resonances of the dielectric disk, while, for larger distances, the surface-plasmon-mode resonances can split. Full article

High-strength naval steel panels were coated by spraying with five commercial antifouling paints. The first set of coated specimens was subjected to electrochemical measurements and the anticorrosion properties of the paints were evaluated under controlled laboratory conditions. A second series of coated samples was statically exposed for nine months in thirteen different harbors, located in the Mediterranean Sea and the Atlantic Ocean, where their in-field antifouling efficiency was determined. The corrosion performance obtained by the electrochemical measurements provides predictions that best match the fouling protection observed at the field sites. The results indicate that the corrosion’s resistivity values determined in the laboratory were in a good agreement with the fouling level observed by examining the sea samples. The reported complementary data show that the laboratory measurements are representative of the sea-field conditions and confirm the originality of the proposed approach, which might lead to the development of innovative low-drag antifouling coatings for the hulls of ships, vessels, and speed crafts. Full article

The Earth’s oceans are the final resting place of anthropogenic wastes, mainly plastics, metals, rubber, and fabrics, in order of decreasing abundance. On reaching the sea and the benthos, most of these have assumed fragmented or particulate forms. They become colonized by marine microorganisms and later interact with macroorganisms, leading to potential problems with marine life and the ecosystem. Rapid biodegradation of the polluting materials is a possible, and desirable, result if harmful by-products are not produced or toxic constituents are released. Negative effects are the transport of organisms to other ecosystems, with possible disturbance of the natural biological balance, or transfer of pathogenic organisms. A microbial biofilm can mask unattractive anthropogenic materials, increasing ingestion by marine life, with potentially dangerous results. This article seeks to provide a synthesis of the interactions occurring between oceanic anthropogenic polluting matter in solid and particulate form, and the microbiota present in our seas. It discusses the most important solid and particulate pollutants in the oceans, their sources, adverse effects, interactions with living organisms, mainly microorganisms, and future research for their control. Pollutants included are marine litter (macrodebris), microplastics, engineered nanoparticles, metallic particles, and, finally, sinking particles (“marine snow”) as a potential biodegradation “hot spot”. Full article

Peptide-decorated nanosystems have demonstrated higher stability and improved cellular uptake as compared to bare NPs and appear highly promising in diagnostics and theranostics of cancer. Herein, we discuss the preparation and structural characterization of peptide-functionalized silica/PEG NPs, starting from peptide–block copolymers, prepared in turn by conjugation of the peptides to block copolymers before NP formation. This synthetic design allowed full control of density and composition of peptide surface coverage. Preliminary experiments support the low toxicity of the fluorescent peptide–NPs and their ability of cell internalization. Full article

The geometrical constraints and dimensional tolerances lead to specific design issues of MEMS manipulators for biological applications. The target properties become even more important in the case of in vitro manipulation of cells. Several design solutions have been proposed in the literature, however, some issues related to the thermal heating of microgripper tips and to the electric voltage effects still remain unsolved. This paper reports the design for additive manufacturing (DFAM) of micro-electro mechanical systems (MEMS) microgrippers. The design limitations imposed by the micro-stereolithography fabrication process are considered. The design solution proposed in this study is based on compliant structures and external actuation; this layout provides the potential elimination of the main issues related to cells micro-manipulators represented by the excessive thermal heating and the voltage exposure of samples. The simulation through finite elements method (FEM) models of the structure in terms of force–displacement relation and stress distribution supports the design evolution proposed. Full article