HGF and VEGF-A and Their Receptors Show Expression and Angiogenic Effects on Human Choroidal Endothelial Cells: Implications for Treatments of Neovascular Age-Related Macular Degeneration
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Isolation and Culture
2.2. Immunohistochemistry
2.3. Immunocytochemistry
2.4. Western Blotting
2.5. Flow Cytometry
2.6. Enzyme-Linked Immunosorbent Assay (ELISA)
2.7. Proliferation Assay
2.8. Migration (Wound Closure) Assay
2.9. In Vitro Tubule Formation
2.10. Statistical Analysis
3. Results
3.1. Expression of VEGFR2 and MET in Human Choroid and Choroidal Endothelial Cells
3.2. CEC Secrete High Levels of VEGF and HGF
3.3. HGF Stimulation of CEC Proliferation and Migration
3.4. Effect of HGF on CEC Tubule Formation on Matrigel
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
References
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Stewart, E.A.; Allen, C.L.; Samaranayake, G.J.; Stubington, T.; Akhtar, R.; Branch, M.J.; Amoaku, W.M. HGF and VEGF-A and Their Receptors Show Expression and Angiogenic Effects on Human Choroidal Endothelial Cells: Implications for Treatments of Neovascular Age-Related Macular Degeneration. Int. J. Transl. Med. 2021, 1, 69-82. https://0-doi-org.brum.beds.ac.uk/10.3390/ijtm1010006
Stewart EA, Allen CL, Samaranayake GJ, Stubington T, Akhtar R, Branch MJ, Amoaku WM. HGF and VEGF-A and Their Receptors Show Expression and Angiogenic Effects on Human Choroidal Endothelial Cells: Implications for Treatments of Neovascular Age-Related Macular Degeneration. International Journal of Translational Medicine. 2021; 1(1):69-82. https://0-doi-org.brum.beds.ac.uk/10.3390/ijtm1010006
Chicago/Turabian StyleStewart, Elizabeth A., Claire L. Allen, Govindi J. Samaranayake, Thomas Stubington, Rukhsar Akhtar, Matthew J. Branch, and Winfried M. Amoaku. 2021. "HGF and VEGF-A and Their Receptors Show Expression and Angiogenic Effects on Human Choroidal Endothelial Cells: Implications for Treatments of Neovascular Age-Related Macular Degeneration" International Journal of Translational Medicine 1, no. 1: 69-82. https://0-doi-org.brum.beds.ac.uk/10.3390/ijtm1010006