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Small-Scale Comparative Genomic Analysis of Listeria monocytogenes Isolated from Environments of Salmon Processing Plants and Human Cases in Norway

1
Department of Process Technology, Nofima—Norwegian Institute of Food, Fisheries and Aquaculture Research, Richard Johnsens Gate 4, 4021 Stavanger, Norway
2
Norwegian Institute of Public Health, Lovisenberggata 8, 0456 Oslo, Norway
3
Norwegian Sequencing Centre, Department of Medical Genetics, Oslo University Hospital, 0450 Oslo, Norway
4
Institute of Marine Research, Section for Contaminants and Biohazards, Nordnesgaten 50, 5005 Bergen, Norway
*
Author to whom correspondence should be addressed.
Hygiene 2021, 1(1), 43-55; https://doi.org/10.3390/hygiene1010005
Received: 30 April 2021 / Revised: 7 June 2021 / Accepted: 7 June 2021 / Published: 16 June 2021
(This article belongs to the Section Infectious Disease Epidemiology, Prevention and Control)
Listeria monocytogenes is a food-borne bacterium that give rise to the potentially life-threatening disease listeriosis. Listeriosis has been mandatorily notifiable in Norway since 1991. All clinical L. monocytogenes isolates are sent to the Norwegian Institute of Public Health (NIPH) for typing. Since 2005 Multi-Locus Variable number tandem repeats Analysis (MLVA) has been used for typing but was recently replaced by whole genome sequencing using core genome Multi-Locus Sequence Typing (cgMLST). In the present study, L. monocytogenes isolates collected at salmon processing plants in Norway in 2007 (n = 12) and 2015 (n = 14) were first subject to MLVA. Twelve clinical L. monocytogenes isolates with matching MLVA profile and sampling time were selected from the strain collection at NIPH. Twenty-one isolates from the salmon processing plants and all clinical isolates (n = 12) were whole genome sequenced and compared using cgMLST and in silico detection of virulence genes. cgMLST revealed four pairs of environmental–human isolates with ≤10 allelic differences over 1708 genes, indicating that they may be assigned as clonal, with the implication that they are descended from the same recent ancestor. No relevant difference in carriage of virulence genes was found between environmental or human isolates. The present study shows that L. monocytogenes strains that genetically resemble contemporary isolates from human listeriosis circulate in Norwegian salmon slaughterhouses, and carry the same virulence genes. View Full-Text
Keywords: food-borne zoonoses; food safety; infectious disease; Listeria; public health; salmon; whole genome sequencing food-borne zoonoses; food safety; infectious disease; Listeria; public health; salmon; whole genome sequencing
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MDPI and ACS Style

Løvdal, T.; Brandal, L.T.; Sundaram, A.Y.M.; Naseer, U.; Roth, B.; Lunestad, B.T. Small-Scale Comparative Genomic Analysis of Listeria monocytogenes Isolated from Environments of Salmon Processing Plants and Human Cases in Norway. Hygiene 2021, 1, 43-55. https://0-doi-org.brum.beds.ac.uk/10.3390/hygiene1010005

AMA Style

Løvdal T, Brandal LT, Sundaram AYM, Naseer U, Roth B, Lunestad BT. Small-Scale Comparative Genomic Analysis of Listeria monocytogenes Isolated from Environments of Salmon Processing Plants and Human Cases in Norway. Hygiene. 2021; 1(1):43-55. https://0-doi-org.brum.beds.ac.uk/10.3390/hygiene1010005

Chicago/Turabian Style

Løvdal, Trond, Lin T. Brandal, Arvind Y.M. Sundaram, Umaer Naseer, Bjørn Roth, and Bjørn T. Lunestad 2021. "Small-Scale Comparative Genomic Analysis of Listeria monocytogenes Isolated from Environments of Salmon Processing Plants and Human Cases in Norway" Hygiene 1, no. 1: 43-55. https://0-doi-org.brum.beds.ac.uk/10.3390/hygiene1010005

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