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Journals
Antibiotics
Special Issues
Strategies against Bacterial Biofilm Formation
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Strategies against Bacterial Biofilm Formation

A special issue of Antibiotics (ISSN 2079-6382). This special issue belongs to the section "Antibiofilm Strategies".

Deadline for manuscript submissions: closed (15 April 2022) | Viewed by 13205

Special Issue Editors

Dr. Fany Reffuveille

E-Mail Website
Guest Editor
Université de Reims Champagne-Ardenne, EA4691 BIOS lab, Reims, France
Interests: biofilm; bone and joint infections; antibiofilm; internalization
Special Issues, Collections and Topics in MDPI journals
Dr. Marius Colin

E-Mail Website
Guest Editor
Université de Reims Champagne-Ardenne, EA4691 BIOS lab, Reims, France
Interests: Antibacterial strategies; Biofilm; Bone and Joint infections; antibiofilm

Special Issue Information

Dear Colleagues,

Biofilm is a mysterious multicellular bacterial form of life that has not revealed all its secrets.

It represents an adaptive and quite common bacterial response to several stress factors, which confer an important protection to bacteria. Biofilm formation starts with bacterial adhesion on a surface, further reinforced by matrix synthesis to finally reach a complex structure. Biological tissues such as bones, lungs or even implanted devices are frequently the siege of biofilm colonization. Furthermore, biofilm implication in numerous diseases is regularly reevaluated upwards. As biofilms are tolerant to any known antibiotics, they represent a major issue for public health. Thus, there is an urgent need to develop adapted strategies to inhibit, perturb or destruct this structure to efficiently fight against biofilm infection.

This Special Issue seeks manuscripts that focus on the development of antibiofilm strategies: chemical or natural compounds, physical techniques, functionalized materials or other innovative antibiofilm processes are welcomed.

Potential topics include but are not limited to:

  • Biofilms mechanisms;
  • Identification of bacterial targets to fight against biofilm;
  • Antibiofilm molecules;
  • Synergy of antimicrobial and antibiofilm molecules;
  • In vitro or in vivo biofilm models development.

Dr. Fany Reffuveille
Dr. Marius Colin
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Antibiotics is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2900 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • biofilm
  • matrix
  • antiadhesive
  • antibiofilm
  • antibiotic tolerance

Published Papers (4 papers)

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Research

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10 pages, 1627 KiB  
Article
A qnrD-Plasmid Promotes Biofilm Formation and Class 1 Integron Gene Cassette Rearrangements in Escherichia coli
by Anamaria Babosan, Margaux Gaschet, Anaëlle Muggeo, Thomas Jové, David Skurnik, Marie-Cécile Ploy, Christophe de Champs, Fany Reffuveille and Thomas Guillard
Antibiotics 2022, 11(6), 715; https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics11060715 - 26 May 2022
Cited by 2 | Viewed by 1862
Abstract
Bacteria within biofilms may be exposed to sub-minimum inhibitory concentrations (sub-MICs) of antibiotics. Cell-to-cell contact within biofilms facilitates horizontal gene transfers and favors induction of the SOS response. Altogether, it participates in the emergence of antibiotic resistance. Aminoglycosides at sub-MICs can induce the [...] Read more.
Bacteria within biofilms may be exposed to sub-minimum inhibitory concentrations (sub-MICs) of antibiotics. Cell-to-cell contact within biofilms facilitates horizontal gene transfers and favors induction of the SOS response. Altogether, it participates in the emergence of antibiotic resistance. Aminoglycosides at sub-MICs can induce the SOS response through NO accumulation in E. coli carrying the small plasmid with the quinolone resistance qnrD gene (pDIJ09-518a). In this study, we show that in E. coli pDIJ09-518a, the SOS response triggered by sub-MICs of aminoglycosides has important consequences, promoting genetic rearrangement in class 1 integrons and biofilm formation. We found that the integrase expression was increased in E. coli carrying pDIJ09-518a in the presence of tobramycin, which was not observed for the WT isogenic strain that did not carry the qnrD-plasmid. Moreover, we showed that biofilm production was significantly increased in E. coli WT/pDIJ09-518a compared to the WT strain. However, such a higher production was decreased when the Hmp-NO detoxification pathway was fully functional by overexpressing Hmp. Our results showing that a qnrD-plasmid can promote biofilm formation in E. coli and potentiate the acquisition and spread of resistance determinants for other antibiotics complicate the attempts to counteract antibiotic resistance and prevention of biofilm development even further. We anticipate that our findings emphasize the complex challenges that will impact the decisions about antibiotic stewardship, and other decisions related to retaining antibiotics as effective drugs and the development of new drugs. Full article
(This article belongs to the Special Issue Strategies against Bacterial Biofilm Formation)
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11 pages, 1335 KiB  
Article
Prophylactic Antibiofilm Activity of Antibiotic-Loaded Bone Cements against Gram-Negative Bacteria
by Andréa Cara, Tristan Ferry, Frédéric Laurent and Jérôme Josse
Antibiotics 2022, 11(2), 137; https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics11020137 - 21 Jan 2022
Cited by 7 | Viewed by 2645
Abstract
Gram-negative bacilli can be responsible for prosthetic joint infection (PJI) even if staphylococci are the main involved pathogens. Gram-negative PJIs (GN-PJI) are considered difficult-to-treat infections due to the increase in antimicrobial resistance and biofilm formation. To minimize the risk of infection in cases [...] Read more.
Gram-negative bacilli can be responsible for prosthetic joint infection (PJI) even if staphylococci are the main involved pathogens. Gram-negative PJIs (GN-PJI) are considered difficult-to-treat infections due to the increase in antimicrobial resistance and biofilm formation. To minimize the risk of infection in cases of arthroplasties with cemented prosthesis, bone cement can be loaded with antibiotics, especially gentamicin. In this study, we aimed to compare the prophylactic antibiofilm activity of ready-to-use antibiotic-loaded bone cements (ALBC), already commercialized or new prototypes. We compared ALBCs containing gentamicin alone, gentamicin plus vancomycin, gentamicin plus clindamycin, gentamicin plus Fosfomycin, and fosfomycin alone, to plain cement (no antibiotic); these comparisons were conducted to investigate the biofilm formation of three strains of Escherichia coli, three strains of Pseudomonas aeruginosa and two strains of Klebsiella pneumoniae, with or without specific resistance to gentamicin or fosfomycin. We reported that ALBC containing gentamicin and clindamycin (COPAL G+C) seems to be the most interesting ALBC of our tested panel for the prevention of biofilm formation by gentamicin-susceptible strains, even if clindamycin is not effective against Gram-negative bacteria. However, gentamicin-resistant strains are still a problem, and further studies are needed to identify an antibiotic to associate with gentamicin for an efficient dual ALBC against Gram-negative bacteria. Full article
(This article belongs to the Special Issue Strategies against Bacterial Biofilm Formation)
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9 pages, 1450 KiB  
Article
Synergy between Indoloquinolines and Ciprofloxacin: An Antibiofilm Strategy against Pseudomonas aeruginosa
by Emilie Charpentier, Ludovic Doudet, Ingrid Allart-Simon, Marius Colin, Sophie C. Gangloff, Stéphane Gérard and Fany Reffuveille
Antibiotics 2021, 10(10), 1205; https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics10101205 - 04 Oct 2021
Cited by 7 | Viewed by 1793
Abstract
Antibiotic treatments can participate in the formation of bacterial biofilm in case of under dosage. The interest of indoloquinoline scaffold for drug discovery incited us to study the preparation of new indolo [2,3-b]quinoline derivatives by a domino radical process. We tested [...] Read more.
Antibiotic treatments can participate in the formation of bacterial biofilm in case of under dosage. The interest of indoloquinoline scaffold for drug discovery incited us to study the preparation of new indolo [2,3-b]quinoline derivatives by a domino radical process. We tested the effect of two different “indoloquinoline” molecules (Indol-1 and Indol-2) without antimicrobial activity, in addition to ciprofloxacin, on biofilm formation thanks to crystal violet staining and enumeration of adhered bacteria. This association of ciprofloxacin and Indol-1 or Indol-2 attenuated the formation of biofilm up to almost 80% compared to ciprofloxacin alone, or even prevented the presence of adhered bacteria. In conclusion, these data prove that the association of non-antimicrobial molecules with an antibiotic can be a solution to fight against biofilm and antibiotic resistance emergence. Full article
(This article belongs to the Special Issue Strategies against Bacterial Biofilm Formation)
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Review

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20 pages, 2273 KiB  
Review
Evidence on the Use of Mouthwash for the Control of Supragingival Biofilm and Its Potential Adverse Effects
by Shoji Takenaka, Maki Sotozono, Naoto Ohkura and Yuichiro Noiri
Antibiotics 2022, 11(6), 727; https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics11060727 - 28 May 2022
Cited by 11 | Viewed by 5859
Abstract
Antimicrobial mouthwash improves supragingival biofilm control when used in conjunction with mechanical removal as part of an oral hygiene routine. Mouthwash is intended to suppress bacterial adhesion during biofilm formation processes and is not aimed at mature biofilms. The most common evidence-based effects [...] Read more.
Antimicrobial mouthwash improves supragingival biofilm control when used in conjunction with mechanical removal as part of an oral hygiene routine. Mouthwash is intended to suppress bacterial adhesion during biofilm formation processes and is not aimed at mature biofilms. The most common evidence-based effects of mouthwash on the subgingival biofilm include the inhibition of biofilm accumulation and its anti-gingivitis property, followed by its cariostatic activities. There has been no significant change in the strength of the evidence over the last decade. A strategy for biofilm control that relies on the elimination of bacteria may cause a variety of side effects. The exposure of mature oral biofilms to mouthwash is associated with several possible adverse reactions, such as the emergence of resistant strains, the effects of the residual structure, enhanced pathogenicity following retarded penetration, and ecological changes to the microbiota. These concerns require further elucidation. This review aims to reconfirm the intended effects of mouthwash on oral biofilm control by summarizing systematic reviews from the last decade and to discuss the limitations of mouthwash and potential adverse reactions to its use. In the future, the strategy for oral biofilm control may shift to reducing the biofilm by detaching it or modulating its quality, rather than eliminating it, to preserve the benefits of the normal resident oral microflora. Full article
(This article belongs to the Special Issue Strategies against Bacterial Biofilm Formation)
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