Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
5.5
4.8
Journals
Antibiotics
Special Issues
New and Innovative Applications of Antimicrobial Photodynamic Therapy
share announcement

New and Innovative Applications of Antimicrobial Photodynamic Therapy

A special issue of Antibiotics (ISSN 2079-6382).

Deadline for manuscript submissions: closed (31 March 2021) | Viewed by 21992

Special Issue Editor

Dr. Kristjan Plaetzer

E-Mail Website
Guest Editor
Laboratory of Photodynamic Inactivation of Microorganisms, Department of Biosciences, University of Salzburg, Salzburg, Austria
Interests: antimicrobial photodynamic therapy; cold plasma; plant pathogens; food safety
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Antimicrobial photodynamic therapy (aPDT) is based on the light-induced and photosensitizer-mediated overproduction of reactive oxygen species to kill a wide spectrum of microorganisms irrespective of their resistance to conventional treatment. Motivated by the success of aPDT in human medicine, novel and very promising applications of this antimicrobial approach are constantly identified and discovered. Recent developments include photodynamic decontamination of food based on edible photosensitizers, photoactive and self-disinfecting materials and textiles, photo-insecticides, and application of aPDT in plant protection against pathogens. As microorganisms trouble humans in all habitats, nearly no limits are set to imagination in terms of further expanding the scope of applications. We invite investigators to contribute original research papers as well as review articles to this Special Issue that will stimulate the efforts to widen the application spectrum of the antimicrobial photodynamic approach.

Potential topics include but are not limited to:
- Antiviral and antifungal photodynamic therapy;
- Application of aPDT in plant protection against pathogens;
- Photodynamic Decontamination for increased food safety;
- Disinfection of surfaces and devices by photodynamic procedures;
- Photo-insecticides;
- aPDT treatment of aquacultures;
- Application of aPDT in veterinary medicine;
- Self-disinfecting photoactive textiles;
- New light sources tailored for aPDT.

PD Dr. Kristjan Plaetzer
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Antibiotics is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2900 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • antimicrobial photodynamic therapy (aPDT)
  • plant pathogens
  • food safety
  • photo-insecticides
  • aquacultures

Published Papers (7 papers)

Download All Papers
Order results
Result details
Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:

Research

14 pages, 3513 KiB  
Article
Photofungizides Based on Curcumin and Derivates Thereof against Candida albicans and Aspergillus niger
by Barbara Schamberger and Kristjan Plaetzer
Antibiotics 2021, 10(11), 1315; https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics10111315 - 28 Oct 2021
Cited by 5 | Viewed by 1934
Abstract
Fungal infections in humans, contamination of food and structural damage to buildings by fungi are associated with high costs for the general public. In addition, the increase in antifungal resistance towards conventional treatment raises the demand for new fungicidal methods. Here, we present [...] Read more.
Fungal infections in humans, contamination of food and structural damage to buildings by fungi are associated with high costs for the general public. In addition, the increase in antifungal resistance towards conventional treatment raises the demand for new fungicidal methods. Here, we present the antifungal use of Photodynamic Inactivation (PDI) based on the natural photosensitizer curcumin and a water-soluble positively charged derivative thereof (SA-CUR 12a) against two different model organisms; Candida albicans grown in a liquid culture and photo treated with a 435 nm LED light followed by counting of the colony-forming units and photoinactivation of tissue-like hyphal spheres of Aspergillus niger (diameter ~5 mm) with subsequent monitoring of colony growth. Curcumin (50 µM, no incubation period, i.p.) supplemented with 10% or 0.5% DMSO as well as SA-CUR 12a (50 µM no i.p or 5 min i.p.) triggered a photoantifungal effect of >4 log units towards C. albicans. At 100 µM, SA-CUR 12a (0 min or 5 min i.p.) achieved a reduction of >6 log units. Colonies of A. niger shrunk significantly during PDI treatment. Photoinactivation with 50 µM or 100 µM curcumin (+0.5% DMSO) resulted in complete growth inhibition. PDI using 20, 50 or 100 µM SA-CUR 12a (with or without 10% DMSO) also showed a significant reduction in colony area compared to the control after 48 h, although less pronounced compared to curcumin. In summary, PDI using curcumin or SA-CUR 12a against C. albicans or A. niger is a promising alternative to currently used fungicides, with the advantage of being very unlikely to induce resistance. Full article
(This article belongs to the Special Issue New and Innovative Applications of Antimicrobial Photodynamic Therapy)
►▼ Show Figures

Figure 1

17 pages, 4016 KiB  
Article
Efficiency of Antimicrobial Photodynamic Therapy with Photodithazine® on MSSA and MRSA Strains
by Beatriz Müller Nunes Souza, Juliana Guerra Pinto, André Henrique Correia Pereira, Alejandro Guillermo Miñán and Juliana Ferreira-Strixino
Antibiotics 2021, 10(7), 869; https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics10070869 - 17 Jul 2021
Cited by 9 | Viewed by 2857
Abstract
Staphylococccus aureus is a ubiquitous and opportunistic bacteria associated with high mortality rates. Antimicrobial photodynamic therapy (aPDT) is based on the application of a light source and a photosensitizer that can interact with molecular oxygen, forming Reactive Oxygen Species (ROS) that result in [...] Read more.
Staphylococccus aureus is a ubiquitous and opportunistic bacteria associated with high mortality rates. Antimicrobial photodynamic therapy (aPDT) is based on the application of a light source and a photosensitizer that can interact with molecular oxygen, forming Reactive Oxygen Species (ROS) that result in bacterial inactivation. This study aimed to analyze, in vitro, the action of aPDT with Photodithazine® (PDZ) in methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) strains. The strains were incubated with PDZ at 25, 50, 75, and 100 mg/L for 15 min and irradiated with fluences of 25, 50, and 100 J/cm2. The internalization of PDZ was evaluated by confocal microscopy, the bacterial growth by counting the number of colony-forming units, as well as the bacterial metabolic activity post-aPDT and the production of ROS. In both strains, the photosensitizer was internalized; the production of ROS increased when the aPDT was applied; there was a bacterial reduction compared to the control at all the evaluated fluences and concentrations; and, in most parameters, it was obtained complete inactivation with significant difference (p < 0.05). The implementation of aPDT with PDZ in clinical strains of S. aureus has resulted in its complete inactivation, including the MRSA strains. Full article
(This article belongs to the Special Issue New and Innovative Applications of Antimicrobial Photodynamic Therapy)
►▼ Show Figures

Figure 1

14 pages, 5210 KiB  
Article
Evaluation of the Photodynamic Therapy with Curcumin on L. braziliensis and L. major Amastigotes
by André Henrique Correia Pereira, Luciana Maria Cortez Marcolino, Juliana Guerra Pinto and Juliana Ferreira-Strixino
Antibiotics 2021, 10(6), 634; https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics10060634 - 25 May 2021
Cited by 10 | Viewed by 3958
Abstract
Cutaneous leishmaniasis (CL) is a neglected disease prevalent in tropical countries with the ability to cause skin lesions. Photodynamic therapy (PDT) represents a specific and topical option for the treatment of these lesions. This study evaluated the response of macrophages infected with L. [...] Read more.
Cutaneous leishmaniasis (CL) is a neglected disease prevalent in tropical countries with the ability to cause skin lesions. Photodynamic therapy (PDT) represents a specific and topical option for the treatment of these lesions. This study evaluated the response of macrophages infected with L. braziliensis and L. major to PDT with curcumin. Curcumin concentrations were evaluated in serial dilutions from 500.0 to 7.8 µg/mL using LED (λ = 450 ± 5 nm), with a light dose of 10 J/cm2. The Trypan blue viability test, ultrastructural analysis by scanning electron microscopy (SEM), mitochondrial polarity by Rhodamine 123 (Rho 123), curcumin internalization by confocal microscopy, and counting of the recovered parasites after the PDT treatment were performed. The lowest concentrations of curcumin (15.6 and 7.8 µg/mL) presented photodynamic inactivation. Cell destruction and internalization of curcumin in both macrophages and intracellular parasites were observed in microscopy techniques. In addition, an increase in mitochondrial membrane polarity and a decrease in the number of parasites recovered was observed in the PDT groups. This study indicates that PDT with curcumin has the potential to inactivate infected macrophages and might act as a basis for future in vivo studies using the parameters herein discussed. Full article
(This article belongs to the Special Issue New and Innovative Applications of Antimicrobial Photodynamic Therapy)
►▼ Show Figures

Figure 1

13 pages, 1493 KiB  
Article
Development of Phenalenone-Triazolium Salt Derivatives for aPDT: Synthesis and Antibacterial Screening
by Jérémy Godard, Dáire Gibbons, Stéphanie Leroy-Lhez, René M. Williams, Nicolas Villandier, Tan-Sothéa Ouk, Frédérique Brégier and Vincent Sol
Antibiotics 2021, 10(6), 626; https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics10060626 - 24 May 2021
Cited by 11 | Viewed by 2715
Abstract
The increasing number of hospital-acquired infections demand the development of innovative antimicrobial treatments. Antimicrobial photodynamic therapy (aPDT) is a versatile technique which relies on the production of reactive oxygen species (ROS) generated by light-irradiated photosensitizers (PS) in the presence of oxygen (O2 [...] Read more.
The increasing number of hospital-acquired infections demand the development of innovative antimicrobial treatments. Antimicrobial photodynamic therapy (aPDT) is a versatile technique which relies on the production of reactive oxygen species (ROS) generated by light-irradiated photosensitizers (PS) in the presence of oxygen (O2). 1H-Phenalen-1-one is a very efficient photosensitizer known for its high singlet oxygen quantum yield and its antimicrobial potential in aPDT when covalently bound to quaternary ammonium groups. Triazolium salts are stable aromatic quaternary ammonium salts that recently appeared as interesting moieties endowed with antimicrobial activities. The coupling between phenalenone and triazolium groups bearing various substituents was realized by copper-catalyzed azide-alkyne cycloaddition followed by alkylation with methyl iodide or 2-(bromomethyl)-1H-phenalen-1-one. As expected, most of the compounds retained the initial singlet oxygen quantum yield, close to unity. Minimum inhibitory concentrations (MIC) of 14 new phenalenone-triazolium salt derivatives and 2 phenalenone-triazole derivatives were determined against 6 bacterial strains (Gram-negatives and Gram-positives species). Most of these PS showed significant photoinactivation activities, the strongest effects being observed against Gram-positive strains with as low as submicromolar MIC values. Full article
(This article belongs to the Special Issue New and Innovative Applications of Antimicrobial Photodynamic Therapy)
►▼ Show Figures

Figure 1

16 pages, 2484 KiB  
Article
Phenothiazinium Photosensitizers Associated with Silver Nanoparticles in Enhancement of Antimicrobial Photodynamic Therapy
by Glaucia Rigotto Caruso, Ludmilla Tonani, Priscyla Daniely Marcato and Marcia Regina von Zeska Kress
Antibiotics 2021, 10(5), 569; https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics10050569 - 12 May 2021
Cited by 7 | Viewed by 2378
Abstract
Antimicrobial photodynamic therapy (APDT) and silver nanoparticles (AgNPs) are known as promising alternatives for the control of microorganisms. This study aims to evaluate the antifungal activity of APDT, particularly by using the association of low concentrations of phenothiazinium photosensitizers (PS) methylene blue (MB), [...] Read more.
Antimicrobial photodynamic therapy (APDT) and silver nanoparticles (AgNPs) are known as promising alternatives for the control of microorganisms. This study aims to evaluate the antifungal activity of APDT, particularly by using the association of low concentrations of phenothiazinium photosensitizers (PS) methylene blue (MB), new methylene blue N (NMBN), and new methylene blue N Zinc (NMBN-Zn) in association with biosynthesized AgNPs. The AgNPs were characterized by UV-Vis spectrophotometry, transmission electron microscopy, and the dynamic light scattering method. The minimum inhibitory concentration of compounds in APDT against Candida albicans and Fusarium keratoplasticum was obtained and the Fractional Inhibitory Concentration Index determined the antifungal effect. The toxicity of compounds and associations in APDT were evaluated in Galleria mellonella. The AgNPs presented a surface plasmon band peak at 420 nm, hydrodynamic diameter of 86.72 nm, and zeta potential of −28.6 mV. AgNPs-PS showed a wider and displaced plasmon band peak due to PS ligands on the surface and decreased zeta potential. AgNPs-NMBN and AgNPs-NMBN-Zn associations presented synergistic effect in APDT with 15 J cm−2 against both fungi and did not show toxicity to G. mellonella. Hence, the enhancement of antifungal activity with low concentrations of compounds and absence of toxicity makes APDT with AgNPs-PS a promising therapeutic alternative for fungal infections. Full article
(This article belongs to the Special Issue New and Innovative Applications of Antimicrobial Photodynamic Therapy)
►▼ Show Figures

Figure 1

26 pages, 4463 KiB  
Article
Photophysical and Antibacterial Properties of Porphyrins Encapsulated inside Acetylated Lignin Nanoparticles
by Nidia Maldonado-Carmona, Tan-Sothea Ouk, Nicolas Villandier, Claude Alain Calliste, Mário J. F. Calvete, Mariette M. Pereira and Stéphanie Leroy-Lhez
Antibiotics 2021, 10(5), 513; https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics10050513 - 30 Apr 2021
Cited by 18 | Viewed by 2533
Abstract
Lignin has recently attracted the attention of the scientific community, as a suitable raw material for biomedical applications. In this work, acetylated lignin was used to encapsulate five different porphyrins, aiming to preserve their photophysical properties, and for further use as antibacterial treatment. [...] Read more.
Lignin has recently attracted the attention of the scientific community, as a suitable raw material for biomedical applications. In this work, acetylated lignin was used to encapsulate five different porphyrins, aiming to preserve their photophysical properties, and for further use as antibacterial treatment. The obtained nanoparticles were physically characterized, through dynamic light scattering size measurement, polydispersity index and zeta potential values. Additionally, the photophysical properties of the nanoparticles, namely UV-vis absorption, fluorescence emission, singlet oxygen production and photobleaching, were compared with those of the free porphyrins. It was found that all the porphyrins were susceptible to encapsulation, with an observed decrease in their fluorescence quantum yield and singlet oxygen production. These nanoparticles were able to exert an effective photodynamic bactericide effect (blue-LED light, 450–460 nm, 15 J/cm2) on Staphylococcus aureus and Escherichia coli. Furthermore, it was achieved a photodynamic bactericidal activity on an encapsulated lipophillic porphyrin, where the free porphyrin failed to diminish the bacterial survival. In this work it was demonstrated that acetylated lignin encapsulation works as a universal, cheap and green material for the delivery of porphyrins, while preserving their photophysical properties. Full article
(This article belongs to the Special Issue New and Innovative Applications of Antimicrobial Photodynamic Therapy)
►▼ Show Figures

Graphical abstract

14 pages, 12980 KiB  
Article
Photodynamic Therapy for the Treatment of Infected Leg Ulcers—A Pilot Study
by Magdalena Krupka, Andrzej Bożek, Dorota Bartusik-Aebisher, Grzegorz Cieślar and Aleksandra Kawczyk-Krupka
Antibiotics 2021, 10(5), 506; https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics10050506 - 29 Apr 2021
Cited by 12 | Viewed by 3215
Abstract
Chronic and infected leg ulcers (LUs) are painful, debilitating, resistant to antibiotics, and immensely reduce a patient’s quality of life. The purpose of our study was to demonstrate the efficacy of photodynamic therapy (PDT) for the treatment of infected chronic LUs. Patients were [...] Read more.
Chronic and infected leg ulcers (LUs) are painful, debilitating, resistant to antibiotics, and immensely reduce a patient’s quality of life. The purpose of our study was to demonstrate the efficacy of photodynamic therapy (PDT) for the treatment of infected chronic LUs. Patients were randomized into two experimental groups: the first group received 5-aminolevulinic acid photodynamic therapy (ALA-PDT) (10 patients), and the second group of 10 patients received local octenidine dihydrochloride (Octenilin gel) exposed to a placebo light source with an inserted filter that mimiced red light. In the PDT group, we used 20% ALA topically applied for 4 hrs and irradiation from a Diomed laser source with a wavelength of 630 nm at a fluency of 80 J/cm2. ALA-PDT was performed 10 times during a 14-day hospitalization in 10 patients of both sexes aged 40–85 years with chronic leg ulcers. Treatments were carried out at 3-week intervals for 3–5 cycles. At 8-month follow-up with the PDT group, complete remission (CR) was obtained in four patients (40%), partial response (>50% reduction in ulcer diameter) in four patients (40%), and no response in two patients (20%) who additionally developed deterioration of the local condition with swelling, erythema, and inflammation. To assess the degree of pain during the trials, we used a numeric rating scale (NRS). From the preliminary results obtained, we concluded that PDT can be used to treat leg ulcers as a minimally invasive and effective method with no serious side effects, although further studies on a larger group of patients with LUs are warranted. Full article
(This article belongs to the Special Issue New and Innovative Applications of Antimicrobial Photodynamic Therapy)
►▼ Show Figures

Figure 1

Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:
 
Displaying articles 1-7
Back to TopTop