Tracing Back Multidrug-Resistant Bacteria in Fresh Produce Production

A special issue of Antibiotics (ISSN 2079-6382). This special issue belongs to the section "Mechanism and Evolution of Antibiotic Resistance".

Deadline for manuscript submissions: closed (30 September 2021) | Viewed by 2549

Special Issue Editor


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Guest Editor
Department of Life Sciences, Albstadt-Sigmaringen University, 72488 Sigmaringen, Germany
Interests: food microbiology; antibiotic resistances; microbial diversity; analytical microbiology

Special Issue Information

Dear Colleagues,

Antibiotics used in human and veterinary medicine are rapidly losing their effectiveness owing to an increase in antibiotic-resistant bacteria and antibiotic resistance mechanisms, including multidrug resistance, worldwide. Antibiotic resistance genes are not limited to bacteria hosted by humans and animals but are also present in bacteria in food and its production environment, i.e., in relation to fresh produce and herbs, surface and irrigation water, and soil and organic fertilizers used in agriculture.

While an important source of food, the abovementioned plant products consumed in their raw state may also be carriers of multidrug-resistant bacteria. Unlike cooked products, these bacteria are not killed during preparation. This is why preventive measures to minimize the spread of multidrug-resistant bacteria from the environment to agriculture and food and the consumer in the first place are important. This is true for not only disease-causing pathogens that can affect humans but also environmental bacteria that are in themselves harmless. These play a role in the development of resistant pathogens, since bacteria can exchange genetic material between themselves.

A great deal of research continues to reveal how multidrug-resistant bacteria are transferred from environmental sources to commercially grown plants and the consumer using state-of-the-art approaches in microbial source tracking. The application of “omics” technologies to close the knowledge gaps that exist at the interfaces between environmental factors, agricultural practices, and the food that we eat by characterizing resistomes, microbiomes, and plasmidomes continues to reinforce this concept. This Special Issue seeks manuscript submissions that further our understanding of multidrug-resistant bacteria in fresh produce production.

Prof. Dr. David Drissner
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Antibiotics is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2900 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Prof. Dr. David Drissner
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Antibiotics is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2900 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • antibiotic resistant bacteria
  • antibiotic resistance
  • multidrug-resistance
  • fresh produce
  • herbs
  • water
  • soil
  • organic fertilisers
  • resistome
  • microbiome
  • plasmidome
  • horizontal gene transfer
  • microbial source tracking

Published Papers (1 paper)

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12 pages, 1330 KiB  
Article
Tracing Antibiotic Resistance Genes along the Irrigation Water Chain to Chive: Does Tap or Surface Water Make a Difference?
by Maria-Theresia Gekenidis, Fiona Walsh and David Drissner
Antibiotics 2021, 10(9), 1100; https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics10091100 - 11 Sep 2021
Cited by 3 | Viewed by 2145
Abstract
Irrigation water is well known as potential source of pathogens in fresh produce. However, its role in transferring antibiotic resistance determinants is less well investigated. Therefore, we analyzed the contribution of surface and tap water to the resistome of overhead-irrigated chive plants. Field-grown [...] Read more.
Irrigation water is well known as potential source of pathogens in fresh produce. However, its role in transferring antibiotic resistance determinants is less well investigated. Therefore, we analyzed the contribution of surface and tap water to the resistome of overhead-irrigated chive plants. Field-grown chive was irrigated with either surface water (R-system) or tap water (D-system), from planting to harvest. Water along the two irrigation chains as well as the respective plants were repeatedly sampled and screened for 264 antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs), using high-capacity qPCR. Differentially abundant (DA) ARGs were determined by comparing the two systems. On R-chive, β-lactam ARGs, multidrug-resistance (MDR) determinants, and MGEs were most abundant, while D-chive featured DA ARGs from the vancomycin class. Diversity and number of DA ARGs was the highest on young chives, strongly diminished at harvest, and increased again at the end of shelf life. Most ARGs highly enriched on R- compared to D-chive were also enriched in R- compared to D-sprinkler water, indicating that water played a major role in ARG enrichment. Of note, blaKPC was detected at high levels in surface water and chive. We conclude that water quality significantly affects the resistome of the irrigated produce. Full article
(This article belongs to the Special Issue Tracing Back Multidrug-Resistant Bacteria in Fresh Produce Production)
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