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Applied Sciences
Special Issues
Biofilm and Applications in Medicine and Industry
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Biofilm and Applications in Medicine and Industry

A special issue of Applied Sciences (ISSN 2076-3417). This special issue belongs to the section "Applied Biosciences and Bioengineering".

Deadline for manuscript submissions: closed (31 December 2021) | Viewed by 3638

Special Issue Editors

Dr. Adam Junka

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Guest Editor
Department of Pharmaceutical Microbiology and Parasitology, Wroclaw Medical University, Borowska 211 Str., 50-534 Wroclaw, Poland
Interests: bacterial cellulose; antiseptics; dressings; medical biofilms
Special Issues, Collections and Topics in MDPI journals
Prof. Karol Fijałkowski

E-Mail Website
Guest Editor
Department of Microbiology and Biotechnology, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology, 70-310 Szczecin, Poland
Interests: bacterial cellulose; magnetic fields; immobilization; bioprocess engineering; medical biofilms
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Microbial biofilm is one of the most important phenomena observed in our world. The number of studies concerning the impact of these communities of microorganisms on human health and industrial settings is growing exponentially every year, providing fresh insight into the complexity and nature of these fascinating structures. Therefore, we cordially invite you to submit your latest research to a Special Issue of Applied Sciences entitled “Biofilm”. We would like this Special Issue to cover as many biofilm aspects as possible, and therefore, we welcome articles concerning biofilm formation and composition, new biofilm-oriented antimicrobials and methods of biofilm eradication, and methods of biofilm detection and application of biofilm-related products in medicine and industry. We hope that this SPECIAL ISSUE will be a worthwhile body of knowledge bringing us closer to biofilm understanding and management.

Dr. Adam Junka
Prof. Karol Fijałkowski
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Applied Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • biofilm
  • medicine
  • industry
  • diagnostics
  • eradication
  • application

Published Papers (1 paper)

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Research

19 pages, 5757 KiB  
Article
The Novel Quantitative Assay for Measuring the Antibiofilm Activity of Volatile Compounds (AntiBioVol)
by Malwina Brożyna, Anna Żywicka, Karol Fijałkowski, Damian Gorczyca, Monika Oleksy-Wawrzyniak, Karolina Dydak, Paweł Migdał, Bartlomiej Dudek, Marzenna Bartoszewicz and Adam Junka
Appl. Sci. 2020, 10(20), 7343; https://0-doi-org.brum.beds.ac.uk/10.3390/app10207343 - 20 Oct 2020
Cited by 6 | Viewed by 3058
Abstract
Herein, we present a new test, dubbed AntiBioVol, to be used for the quantitative evaluation of antibiofilm activity of volatile compounds in vitro. AntiBioVol is performed in two 24-well plates using a basic microbiological laboratory equipment. To demonstrate AntiBioVol usability, we have scrutinized [...] Read more.
Herein, we present a new test, dubbed AntiBioVol, to be used for the quantitative evaluation of antibiofilm activity of volatile compounds in vitro. AntiBioVol is performed in two 24-well plates using a basic microbiological laboratory equipment. To demonstrate AntiBioVol usability, we have scrutinized the activity of volatilized eucalyptus, tea tree, thyme essential oils, and ethanol (used for method suitability testing) against biofilms of Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans. We have also compared AntiBioVol with the standard disc volatilization method, placing a special stress on evaluating the impact of various technical parameters on the outcomes of the latter method. The obtained results indicate that AntiBioVol allows analyzing the antibiofilm activity of volatile compounds in a high number of repeats and provides semi-quantitative or quantitative results of high repeatability. In comparison to disc volatilization, AntiBioVol is a more space- and cost-effective method that allows analyzing various types of microbial aggregates. Moreover, we have indicated that the possible reasons for the discrepancies in the results obtained by means of the standard disc volatilization method may be related to various parameters of the testing dishes used (height, volume, diameter) and to various volumes of the agar medium applied. In turn, the application of a 24-well plate and a strictly defined AntiBioVol protocol provide a higher control of experimental conditions. Therefore, the application of AntiBioVol may enable an optimization of and introduction of volatile compounds to the fight against infective biofilms. Full article
(This article belongs to the Special Issue Biofilm and Applications in Medicine and Industry)
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