Biofilms in the Spotlight: A Threat That Needs to Be Controlled

A special issue of Applied Sciences (ISSN 2076-3417). This special issue belongs to the section "Food Science and Technology".

Deadline for manuscript submissions: closed (15 January 2022) | Viewed by 2779

Special Issue Editors


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Guest Editor
Departament de Ciència Animal i dels Aliments, Facultat de Veterinària, Universitat Autònoma de Barcelona, Barcelona, Spain
Interests: biofilm; food safety; food microbiology; biosafety; cleaning; disinfection; sanitization; biosecurity
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Guest Editor
Food Hygiene and Inspection Unit, Department of Animal and Food Science, Faculty of Veterinary Sciences, Autonomous University of Barcelona, Bellaterra, Spain
Interests: biofilms; microbiota; foodborne pathogens; disinfectants; resistance; sublethal concentrations; gene expression; food preservation; hygiene; food safety
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The world of microbiology is changing. Consumers and taxpayers are not aware of it, and neither are many of the technicians involved in food safety. Nowadays, there is a silent problem related to microbiology, and particularly, to food microbiology. That is the ability of microorganisms to colonize and subsequently form biofilms on surfaces, which protect them from adverse conditions and make them more resistant than their planktonic free-living counterparts. This is a major concern in the food industry because the presence of biofilms has significant implications for microbial food contamination and, therefore, for the transmission of foodborne diseases.

Little by little, without being aware of it, the presence of pathogenic microorganisms on food products have been increasing. Pathogens such as Escherichia coli Shiga-toxin producers, Listeria monocytogenes, or Yersinia enterocolitica are becoming more frequent. They are microorganisms that are clearly sensitive to common disinfectants, such as sodium hypochlorite or quaternary ammonia, that have already been used in disinfection for several hundreds of years, but which are not eliminated in food facilities after the disinfection process. Is it because of a new resistance to disinfectants? Clearly not—in recommended concentrations, the justification lies in its great capacity to produce biofilms, a very effective form of resistance, which allows them to survive, grow, and colonize large areas. Bacteria’s ability to adhere to industrial surfaces and to subsequently trigger biofilm formation has significant implications within the food industry, especially for their consequences regarding public health and economic productivity. Today, L. monocytogenes is already the pathogen with the highest mortality in Europe and the United States, and one of the main causes of the pathogen contaminating a product is because of cross-contamination from surfaces. Moreover, we cannot forget the presence and interaction between pathogens and spoilage microorganisms, because of the interaction between them, which is essential in order to assure a quick and stable biofilm structure. Perhaps more time and research are needed in order to be able to include these structures as a priority in food control—meanwhile, hundreds of deaths accumulate in the statistics.

Prof. Dr. José Juan Rodríguez-Jerez
Dr. Carolina Ripolles-Avila
Guest Editors

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Keywords

  • Biofilms
  • Cleaning
  • Disinfection
  • Sanitization
  • Food microbiology
  • Food safety
  • Pathogens
  • Food control

Published Papers (1 paper)

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Research

13 pages, 2120 KiB  
Article
Biofilm Formation by Multidrug-Resistant Serotypes of Salmonella Isolated from Fresh Products: Effects of Nutritional and Environmental Conditions
by María-Guadalupe Avila-Novoa, Pedro-Javier Guerrero-Medina, Velia Navarrete-Sahagún, Itzel Gómez-Olmos, Noemí-Yolanda Velázquez-Suárez, Lucia De la Cruz-Color and Melesio Gutiérrez-Lomelí
Appl. Sci. 2021, 11(8), 3581; https://0-doi-org.brum.beds.ac.uk/10.3390/app11083581 - 16 Apr 2021
Cited by 5 | Viewed by 2283
Abstract
Salmonella serotypes can develop biofilms in fresh food products. This study focused on determining the antimicrobial resistance profile and the effects of different growth media and environmental conditions on biofilm formation by multidrug-resistant serotypes of Salmonella. All 49.4% of the Salmonella strains [...] Read more.
Salmonella serotypes can develop biofilms in fresh food products. This study focused on determining the antimicrobial resistance profile and the effects of different growth media and environmental conditions on biofilm formation by multidrug-resistant serotypes of Salmonella. All 49.4% of the Salmonella strains (five serotypes) were multidrug resistant. Assessment of the ability to form biofilms using the crystal violet staining method revealed that 95.6% of the strains of Salmonella were strong biofilm producers in 96-well polystyrene microtiter plates. Overall, 59.3% of the Salmonella strains showed the rdar (red dry and rough colony) morphotype, 2.1% pdar (pink dry and rough colony), 27.4% bdar (brown dry and rough colony) and 10.9% saw (smooth and white colony), at two temperatures (22 and 35 °C). Mono-species biofilms of Salmonella serotypes showed a mean cell density of 8.78 log10 CFU/cm2 ± 0.053 in TSBS (1/20 diluted TSB (tryptic soy broth) + 1% strawberry residues) and 8.43 log10 CFU/cm2 ± 0.050 in TSBA (1/20 diluted TSB + 1% avocado residues) on polypropylene type B (PP) (p < 0.05). In addition, epifluorescence microscopy and scanning electron microscopy (SEM) enabled visualizing the bacteria and extracellular polymeric substances of biofilms on PP. Salmonella form biofilms depending on the serotype of the strains and the environmental conditions. Mono-species biofilms formed by Salmonella serotypes respond to nutrient limitation with the use of simplified culture media such as TSBA and TSBS. Full article
(This article belongs to the Special Issue Biofilms in the Spotlight: A Threat That Needs to Be Controlled)
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