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Applied Sciences
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Dental Regenerative Biology
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Dental Regenerative Biology

A special issue of Applied Sciences (ISSN 2076-3417). This special issue belongs to the section "Applied Dentistry and Oral Sciences".

Deadline for manuscript submissions: closed (20 October 2022) | Viewed by 5741

Special Issue Editors

Prof. Dr. Alexandra Roman

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Leading Guest Editor
Department of Periodontology, Faculty of Dental Medicine, Iuliu Haţieganu University of Medicine and Pharmacy Cluj-Napoca, 400012 Cluj-Napoca, Romania
Interests: periodontal medicine; periodontal regeneration; oral stem cell biology; dental materials; biosafety and tooth-periodontal interactions; animal models for ridge preservation; muco-gingival surgery
Special Issues, Collections and Topics in MDPI journals
Dr. Andrada Soanca

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Guest Editor
Department of Periodontology, Faculty of Dental Medicine, Iuliu Haţieganu University of Medicine and Pharmacy Cluj-Napoca, 400012 Cluj-Napoca, Romania
Interests: periodontal regeneration; periodontal surgery; oral stem cell biology; biomaterials; dental materials; tooth-periodontal interactions; dental adhesion
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues

This Special Issue of Applied Sciences, "Dental Regenerative Biology", invites researchers to submit articles focused on in-depth research on the cellular behaviors and interactions capable of inducing the regeneration of dento-periodontal tissues and on the development of new biomaterials or techniques capable of broadening the stimulative regenerative effects in dentistry. There are no satisfactory answers related to the treatment of the two major oral diseases, dental caries and periodontitis—real public health problems—and finding solutions for the regeneration of lost structures is an acute priority. Aging associated with increased life expectancy encourages us to find solutions and refine the scientific reasoning to improve the outcome of dental procedures. Experimental and theoretical studies in the field of dentistry under the aegis of multidisciplinarity are all encouraged. The aim is to build a community of authors and readers who share the latest ideas in this field and develop future research directions.

We kindly invite you to submit your valuable contributions to this Special Issue.

Prof. Dr. Alexandra Roman
Prof. Dr. Andrada Soanca
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Applied Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • tooth
  • periodontium
  • regeneration
  • healing
  • stem cell
  • cell biology
  • biomaterials
  • biomolecules
  • scaffold
  • membrane
  • periodontitis
  • nanotechnology
  • biocompatibility
  • restorative dentistry
  • periodontology
  • aging

Published Papers (3 papers)

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Research

15 pages, 2715 KiB  
Article
Examination of the Quality of Particulate and Filtered Mandibular Bone Chips for Oral Implants: An In Vitro Study
by Patrick Babczyk, Martin Winter, Claudia Kleinfeld, Andreas Pansky, Christina Oligschleger and Edda Tobiasch
Appl. Sci. 2022, 12(4), 2031; https://0-doi-org.brum.beds.ac.uk/10.3390/app12042031 - 16 Feb 2022
Cited by 1 | Viewed by 1452
Abstract
(1) Background: Autologous bone is supposed to contain vital cells that might improve the osseointegration of dental implants. The aim of this study was to investigate particulate and filtered bone chips collected during oral surgery intervention with respect to their osteogenic potential and [...] Read more.
(1) Background: Autologous bone is supposed to contain vital cells that might improve the osseointegration of dental implants. The aim of this study was to investigate particulate and filtered bone chips collected during oral surgery intervention with respect to their osteogenic potential and the extent of microbial contamination to evaluate its usefulness for jawbone reconstruction prior to implant placement. (2) Methods: Cortical and cortical-cancellous bone chip samples of 84 patients were collected. The stem cell character of outgrowing cells was characterized by expression of CD73, CD90 and CD105, followed by osteogenic differentiation. The degree of bacterial contamination was determined by Gram staining, catalase and oxidase tests and tests to evaluate the genera of the found bacteria (3) Results: Pre-surgical antibiotic treatment of the patients significantly increased viability of the collected bone chip cells. No significant difference in plasticity was observed between cells isolated from the cortical and cortical-cancellous bone chip samples. Thus, both types of bone tissue can be used for jawbone reconstruction. The osteogenic differentiation was independent of the quantity and quality of the detected microorganisms, which comprise the most common bacteria in the oral cavity. (4) Discussion: This study shows that the quality of bone chip-derived stem cells is independent of the donor site and the extent of present common microorganisms, highlighting autologous bone tissue, assessable without additional surgical intervention for the patient, as a useful material for dental implantology. Full article
(This article belongs to the Special Issue Dental Regenerative Biology)
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14 pages, 1543 KiB  
Article
Oral Mesenchymal Stromal Cells in Systemic Sclerosis: Characterization and Response to a Hyaluronic-Acid-Based Biomaterial
by Alina Stanomir, Carmen Mihaela Mihu, Simona Rednic, Cristina Pamfil, Alexandra Roman, Andrada Soancă, Iulia Cristina Micu, Adriana Elena Bulboacă, Ștefan Ioan Stratul, Aurel Popa-Wagner and Emoke Pall
Appl. Sci. 2021, 11(17), 8101; https://0-doi-org.brum.beds.ac.uk/10.3390/app11178101 - 31 Aug 2021
Viewed by 1647
Abstract
Introduction. As oral mesenchymal stromal cells (MSCs) have not, to date, been isolated from systemic sclerosis (SSc) patients, the aim of this in vitro experiment was to characterize gingival MSCs (SScgMSCs) and granulation tissue MSCs (SScgtMSCs) from SSc and to evaluate their functionality [...] Read more.
Introduction. As oral mesenchymal stromal cells (MSCs) have not, to date, been isolated from systemic sclerosis (SSc) patients, the aim of this in vitro experiment was to characterize gingival MSCs (SScgMSCs) and granulation tissue MSCs (SScgtMSCs) from SSc and to evaluate their functionality in comparison to healthy MSCs (hMSCs), in normal or hyaluronic acid (HA) culture media. Materials and Methods. Isolated cells were described by immunophenotyping of surface antigen make-up and by trilineage mesenchymal differentiation capacity. Colony-Forming Unit-Fibroblast (CFU-F) test and migration potential evaluated MSC functionality. Results. All types of MSCs displayed positivity for the following surface markers: CD29, CD73, CD90, CD105, CD44, and CD79a. These cells did not express CD34, CD45, HL-DR, and CD14. Isolated MSCs differentiated into osteoblasts, adipocytes, and chondroblasts. The frequency of CFU-F for SScgtMSCs was significantly lower than that of hMSCs (p = 0.05) and SScgMSCs (p = 0.004) in normal medium, and also markedly lower than that of SScgMSCs (p = 0.09) in HA medium. Following HA exposure, both SScgMSCs and SScgtMSCs migrated significantly less (p = 0.033 and p = 0.005, respectively) than hMSCs. Conclusions. A reduced functionality of MSCs derived from SSc as compared to hMSCs was observed. HA in culture medium appeared to significantly stimulate the migration potential of hMSCs. Full article
(This article belongs to the Special Issue Dental Regenerative Biology)
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11 pages, 1890 KiB  
Article
Acid Dentin Lysates Increase Amelotin Expression in Oral Epithelial Cells and Gingival Fibroblasts
by Jila Nasirzade, Zahra Kargarpour, Layla Panahipour and Reinhard Gruber
Appl. Sci. 2021, 11(12), 5394; https://0-doi-org.brum.beds.ac.uk/10.3390/app11125394 - 10 Jun 2021
Cited by 1 | Viewed by 1920
Abstract
Amelotin (AMTN) is a secretory calcium-binding phosphoprotein controlling the adhesion of epithelial cells to the tooth surface, forming a protective seal against the oral cavity. It can be proposed that signals released upon dentinolysis increase AMTN expression in periodontal cells, thereby helping to [...] Read more.
Amelotin (AMTN) is a secretory calcium-binding phosphoprotein controlling the adhesion of epithelial cells to the tooth surface, forming a protective seal against the oral cavity. It can be proposed that signals released upon dentinolysis increase AMTN expression in periodontal cells, thereby helping to preserve the protective seal. Support for this assumption comes from our RNA sequencing approach showing that gingival fibroblasts exposed to acid dentin lysates (ADL) greatly increased AMTN expression. In the present study, we confirm that acid dentin lysates significantly increase AMTN in gingival fibroblasts and extend this observation towards the epithelial cell lineage by use of the HSC2 oral squamous and TR146 buccal carcinoma cell lines. AMTN immunostaining revealed an intensive signal in the nucleus of HSC2 cells exposed to acid dentin lysates. Acid dentin lysates mediate their effect via the transforming growth factor (TGF)-β type 1 receptor kinase as the antagonist SB431542 abolished the expression of AMTN in the epithelial cells and fibroblasts. Similar to what is known for fibroblasts, acid dentin lysate increased Smad-3 phosphorylation in HSC2 cells. HSC2 cells also respond to the AMTN-stimulating activity of the dentin lysate when adsorbed to gelatin. When simulating regenerative approaches, enamel matrix derivative, TGF-β1, and bone morphogenetic protein-2 also caused a robust increase in SB431542-dependent AMTN expression in HSC2. Taken together, we show here that acid dentin lysate uses the TGF-β-depended signaling pathway to support the AMTN expression in epithelial cells, possibly helping in maintaining the protective seal against the oral cavity. Full article
(This article belongs to the Special Issue Dental Regenerative Biology)
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