Digital Pathology: Current Issues and Trends

A special issue of Applied Sciences (ISSN 2076-3417). This special issue belongs to the section "Applied Biosciences and Bioengineering".

Deadline for manuscript submissions: closed (20 November 2022) | Viewed by 5169

Special Issue Editor


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Guest Editor
Institute of Pathology, Friedrich-Alexander-University, Erlangen-Nürnberg, 91054 Erlangen, Germany
Interests: digital pathology; classical digital image analysis (DIA); deep learning algorithms; neuronal nets; colon cancer; Hirschsprung disease; cytology
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Special Issue Information

Dear Colleagues,

This Special Issue is devoted to the application of digital pathology (DP) solutions as a key method in pathology and biomarker research. In this Special Issue, we focus on classical image analysis and new approaches using AI-based solutions and deep learning algorithms. This should underline our title “current issues and trends” in digital pathology. In any case, the authors should demonstrate that the part of digital pathology is a predominant and relevant method to get results, and it is direct towards the implementation of new ideas. In our opinion, the participation of experts in the field using leading methods in digital pathology is important to guide readers and DIA users. Papers about non-malignant tissue as well as about malignancies are welcome. When AI solutions are used, details of training and validation sets are welcome and we invite you to upload datasets to supplement data. This can be useful to lead new scientists into the huge field of AI solutions and to demonstrate the power of new approaches that should be the basis of this Special Issue. Particularly welcome will be works that validate, also at the experimental level, data using AI and neuronal nets or deep learning algorithms at any stage in digital pathology.

In situ applications (e.g., FISH or CISH) are reconsidered on the same level as bright-field microscopy. Original papers and case reports are welcome. Your work should encourage young scientists to work with digital pathology solutions and bring DIA one step further into future diagnostics.

Dr. Carol Geppert
Guest Editor

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Keywords

  • digital pathology
  • digital image analysis
  • deep learning
  • AI-approach
  • AI-based algorithm
  • classical image analysis
  • digital analysis of immunohistochemistry/biomarkers
  • convolutional neural networks (CNNs)

Published Papers (2 papers)

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Research

13 pages, 394 KiB  
Communication
Pathological Digital Biomarkers: Validation and Application
by Youngjae Song, Kyungmin Kang, Inho Kim and Tae-Jung Kim
Appl. Sci. 2022, 12(19), 9823; https://0-doi-org.brum.beds.ac.uk/10.3390/app12199823 - 29 Sep 2022
Cited by 4 | Viewed by 2405
Abstract
Digital pathology offers powerful tools for biomarker discovery, analysis, and translation. Despite its advantages, the clinical adoption of digital pathology has been slow. A clinical and methodological validation is required for novel digital pathological biomarkers. Four steps are required to validate a novel [...] Read more.
Digital pathology offers powerful tools for biomarker discovery, analysis, and translation. Despite its advantages, the clinical adoption of digital pathology has been slow. A clinical and methodological validation is required for novel digital pathological biomarkers. Four steps are required to validate a novel pathological digital biomarker for clinical use: sample collection and processing, analytical validation, clinical validation, and clinical utility. The digital biomarkers and their diagnostic, monitoring, pharmacodynamic response, predictive, prognostic, safety, and risk assessment applications are discussed. Adopting pathological digital biomarkers can be used in conjunction with other diagnostic technologies to select the most appropriate patient treatment, thereby reducing patient suffering and healthcare costs. Full article
(This article belongs to the Special Issue Digital Pathology: Current Issues and Trends)
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16 pages, 4000 KiB  
Article
Accuracy Analysis of a Next-Generation Tissue Microarray on Various Soft Tissue Samples of Wistar Rats
by Jan-Erik Werry, Stefan Müller, Falk Wehrhan, Carol Geppert, Gesche Frohwitter, Jutta Ries, Peer W. Kämmerer, Tobias Moest, Rainer Lutz, Andi Homm, Marco Kesting and Manuel Weber
Appl. Sci. 2021, 11(12), 5589; https://0-doi-org.brum.beds.ac.uk/10.3390/app11125589 - 17 Jun 2021
Viewed by 1751
Abstract
This study aimed to investigate accuracy in different sectional planes of the TMA Grand Master (3DHISTECH) Workstation in various soft tissue samples collected from Wistar rats. A total of 108 animals were sacrificed and 963 tissue specimens collected from 12 soft-tissue types. A [...] Read more.
This study aimed to investigate accuracy in different sectional planes of the TMA Grand Master (3DHISTECH) Workstation in various soft tissue samples collected from Wistar rats. A total of 108 animals were sacrificed and 963 tissue specimens collected from 12 soft-tissue types. A total of 3307 tissue cores were punched and transferred into 40 recipient TMA blocks. Digital image analysis was performed. Core loss showed a significant correlation with tissue type and was highest in skin tissue (p < 0.001), renal medulla and femoral artery, nerve, and vein bundle (p < 0.01). Overall, 231 of 3307 tissue cores (7.0%) were lost. Hit rate analysis was performed in 1852 punches. The target was hit completely, partially and missed totally by 89.4%, 7.2% and 2.2%. A total of 54.5% of punches had good accuracy with less than 200 µm deviation from the centre of the targeted region and 92.6% less than 500 µm. Accuracy decreases with greater sectional depth. In the deepest sectional plane of roughly 0.5 mm median depth, almost 90% of cores had a deviation below 500 µm. Recommendations for automated TMA creation are given in this article. The ngTMA®-method has proven accurate and reliable in different soft tissues, even in deeper sectional layers. Full article
(This article belongs to the Special Issue Digital Pathology: Current Issues and Trends)
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