Recent Advances and Challenges in Tissue Engineering

A special issue of Applied Sciences (ISSN 2076-3417). This special issue belongs to the section "Applied Biosciences and Bioengineering".

Deadline for manuscript submissions: closed (10 September 2021) | Viewed by 2911

Special Issue Editors


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Guest Editor
Bioengineering Institute of Technology (BIT), Department of Basic Sciences, Universitat Internacional de Catalunya, Barcelona, Spain
Interests: nanomaterials; nanotechnology; polymers; nanoparticles ; quality management; materials science; nanoscience; tissue engineering; materials engineering; nanobiotechnology; nanocomposites; surface science; polymer chemistry; science communication; thermodynamics; quality assurance; nanomedicine; hydrogels; surfactants; colloids; magnetic nanoparticles; micelles; soft matter; nanotoxicology; self-assembly; drug delivery systems; 3D printing

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Guest Editor
Deparment of Organic Chemistry, Universidad de Valladolid, Valladolid, Spain
Interests: tissue engineering; biomaterials; regenerative medicine; material characterization; nanotechnology; organic chemistry; polymeric biomaterials; polymers; biomaterial engineering; nanobiotechnology

Special Issue Information

Dear Colleagues,

Tissue engineering is a multidisciplinary, rapidly evolving field incorporating the principles of fundamental bioengineering and basic biomaterial science to create biological substitutes of native tissues to be of help in the clinical and health applied sciences. Tissue engineering would be solving problems related to human health by growing or assisting in the regeneration of tissues and organs in a collaborative effort.

Despite the tremendous advances in the use of biologically derived scaffolds for several decades, the actual tendency to develop synthetically derived scaffolds is reasonable from clinical, regulatory, and research perspectives. There are many challenges and opportunities ahead in tissue engineering.

The excitement of tissue engineering lies in the challenge of imitating nature, and in the opportunity to witness the outcomes of a discovery or invention, because the interval between conception and clinical realization of a new device or treatment is, in our time, telescoped.

This Special Issue aims to incorporate a summary of up-to-date articles to provide a comprehensive overview of the most recent advances and challenges in tissue engineering.

Prof. Dr. Emilio Castro
Dr. Laura Martín
Guest Editors

Manuscript Submission Information

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Keywords

  • Tissue engineering
  • Biomaterials
  • Scaffolds
  • 3D bioprinting
  • Foreign body reaction
  • Material implantation
  • Inflammatory response
  • Monocytes
  • Macrophages
  • Vascularization
  • Stem cells
  • In vitro
  • In vivo
  • Extracellular matrix
  • Bioreactor
  • 3D cell culture
  • Cell signaling
  • Prosthesis

Published Papers (1 paper)

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Research

19 pages, 3320 KiB  
Article
Human Dental Pulp Stem Cell Osteogenic Differentiation Seeded on Equine Bone Block with Graphene and Melatonin
by Rosa Mancinelli, Ester Sara Di Filippo, Margherita Tumedei, Mariangela Marrone, Antonella Fontana, Valeria Ettorre, Silvia Giordani, Michele Baldrighi, Giovanna Iezzi, Adriano Piattelli and Stefania Fulle
Appl. Sci. 2021, 11(7), 3218; https://0-doi-org.brum.beds.ac.uk/10.3390/app11073218 - 03 Apr 2021
Cited by 6 | Viewed by 2264
Abstract
Equine bone blocks have osteogenic effects promoting bone regeneration with biocompatibility and osteoconductivity capacity. Human dental pulp stem cells (hDPSCs) can differentiate into osteoblasts enhancing biomineralization with such scaffolds. Melatonin is able to improve bone health and mediate bone formation. Collagenated equine bone [...] Read more.
Equine bone blocks have osteogenic effects promoting bone regeneration with biocompatibility and osteoconductivity capacity. Human dental pulp stem cells (hDPSCs) can differentiate into osteoblasts enhancing biomineralization with such scaffolds. Melatonin is able to improve bone health and mediate bone formation. Collagenated equine bone blocks were coated with ammonia-functionalized graphene-oxide (G-N) at two different concentrations (2 μg/mL, G-N2; and 10 μg/mL, G-N10). The homogeneity of G-N coating was checked by Raman spectroscopy, whereas thermogravimetric analysis (TGA) allowed us to quantify the amount of G-N deposited on the blocks. The aim of this study was to investigate in vitro the effect of G-N-coated collagenated equine bone blocks on the proliferation and differentiation of hDPSCs with the addition of a melatonin. This evaluation was determined after 7, 14, and 21 days of culture by the expression of specific microRNAs, RUNX2 and SMAD5 gene expression, osteocalcin levels, and histological analysis. The results showed that equine blocks G-N2 and G-N10 and melatonin gave an optimal cell adhesion as shown by histological analysis, and an increase in the hDPSCs osteogenic potential as confirmed by microRNA and gene expression with an increase in osteocalcin levels. This study suggests that equine bone blocks coated with G-N2 and G-N10 and melatonin promote the osteogenic process. Full article
(This article belongs to the Special Issue Recent Advances and Challenges in Tissue Engineering)
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