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Applied Sciences
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Analytical Techniques for the Determination of Bioactive Food Compounds
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Analytical Techniques for the Determination of Bioactive Food Compounds

A special issue of Applied Sciences (ISSN 2076-3417). This special issue belongs to the section "Food Science and Technology".

Deadline for manuscript submissions: closed (31 December 2022) | Viewed by 5101

Special Issue Editor

Dr. Celia Rodriguez Perez

E-Mail Website
Guest Editor
1. Department of Nutrition and Food Science, University of Granada, Campus of Cartuja, 18011 Granada, Spain
2. Institute of Nutrition and Food Technology ‘José Mataix’, Biomedical Research Center, University of Granada, Avda del Conocimiento sn., 18100 Armilla, Granada, Spain
3. Instituto de Investigación Biosanitaria ibs.GRANADA, 18012 Granada, Spain
Interests: bioactive compounds; functional foods; nutrition; antioxidants; dietary patterns; metabolomics
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Plant-based foods are a good source of bioactive compounds—natural substances that provide health benefits beyond their nutritional value. Due to the link between diet and disease treatment/prevention, there is growing interest in the identification of these compounds in relation to desired activities. Several analytical techniques can be used to identify bioactive compounds in foods (including plant-based foods). Among them, chromatographic (i.e., liquid and gas chromatography) and spectrophotometric complementary techniques are most commonly employed for qualitative/quantitative determination of bioactive compounds and comprehensive food characterization through the use of metabolic fingerprints and metabolite profiling. In addition, capillary electrophoresis and nuclear magnetic resonance can be employed for analytical purposes. This Special Issue will include a collection of research and review articles that detail recent advances in the analysis of bioactive compounds from foods, particularly plant-based foods. We invite authors to submit relevant articles that contribute to an increase in knowledge in this area of research.

Dr. Celia Rodriguez Perez
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Applied Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • liquid chromatography
  • gas chromatography
  • spectrophotometric techniques
  • capillary electrophoresis
  • nuclear magnetic resonance
  • foods
  • plant-based foods
  • fingerprints
  • metabolite profiling

Published Papers (2 papers)

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Research

12 pages, 591 KiB  
Article
Determination of Zn2+ in Solid Pharmaceutical Dosage Forms by Means of Spectrophotometry in Micellar Media: Method Validation
by Luca Liviu Rus, Anca Maria Juncan, Veronica Isabela Crăciun, Adina Frum, Simona-Codruța Hegheș, Anca Butuca, Carmen Maximiliana Dobrea, Adriana Aurelia Chis, Andrei Catalin Muntean, Andreea Loredana Vonica-Ţincu and Claudiu Morgovan
Appl. Sci. 2022, 12(9), 4476; https://0-doi-org.brum.beds.ac.uk/10.3390/app12094476 - 28 Apr 2022
Cited by 2 | Viewed by 1770
Abstract
In this study, a spectrophotometric method for the quantitative determination of zinc in a dietary supplement was validated. Zinc forms a ternary complex with Xylenol Orange and cetylpyridinium chloride in acetate buffer at pH = 5.5. The complex exhibits an absorption maximum at [...] Read more.
In this study, a spectrophotometric method for the quantitative determination of zinc in a dietary supplement was validated. Zinc forms a ternary complex with Xylenol Orange and cetylpyridinium chloride in acetate buffer at pH = 5.5. The complex exhibits an absorption maximum at 560 nm. Linearity (range was 0.5–1.5 µg/mL, and correlation coefficient of the calibration curve was 0.9998), precision (repeatability variation coefficient and reproducibility variation coefficient were 1.57% and 1.77%, respectively), and accuracy (confidence interval of the mean recovery was 98.29–100.55%) of this method fulfil current pharmacopeial requests. Results obtained by spectrophotometric method were statistically compared with ones obtained by atomic absorption spectrometry and good agreement was found. The method can be applied routinely for quality control of solid pharmaceutical dosage forms containing zinc. Full article
(This article belongs to the Special Issue Analytical Techniques for the Determination of Bioactive Food Compounds)
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11 pages, 9717 KiB  
Article
Comprehensive Characterization and Quantification of Antioxidant Compounds in Finger Lime (Citrus australasica L.) by HPLC-QTof-MS and UPLC-MS/MS
by Ramón Aznar, Celia Rodríguez-Pérez and Dilip K. Rai
Appl. Sci. 2022, 12(3), 1712; https://0-doi-org.brum.beds.ac.uk/10.3390/app12031712 - 07 Feb 2022
Cited by 9 | Viewed by 2718
Abstract
Australian finger limes (Citrus australasica L.), an unusual citrus due to its unique pulp with a caviar-like appearance, has reached the global market as a promising source of bioactive compounds that promote health. This research was, therefore, performed to shed light on [...] Read more.
Australian finger limes (Citrus australasica L.), an unusual citrus due to its unique pulp with a caviar-like appearance, has reached the global market as a promising source of bioactive compounds that promote health. This research was, therefore, performed to shed light on the bioactivity and composition of different parts of Citrus australasica L. (peel and pulp). Initial ultrasound-assisted extraction using MeOH:H2O (80:20, v/v) was carried out. After that, four fractions (hexane, ethyl acetate, butanol and water) were generated through liquid–liquid partitioning, and the total phenolic content (TPC) and antioxidant activity were evaluated using the Folin–Ciocalteu and the ferric reducing antioxidant power (FRAP) assays, respectively. The ethyl acetate fraction in the peel, which presented the highest values of TPC and antioxidant activity, was characterized using high-performance liquid chromatography coupled to quadrupole time-of-flight (HPLC-QTof) mass spectrometry. Fifteen compounds were identified, of which seven were characterized for the first time in this matrix. Moreover, ten phenolic compounds were quantified using ultra-performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS). The major compounds in the sample were citric acid, pyrogallol, caffeic acid, coumarin, rutin, naringin, 2-coumaric acid, didymin, naringenin and isorhamnetin, which were found in a range from 2.7 to 8106.7 µg/g sample dry weight. Finally, the results presented in this novel work confirmed that the peel by-product of C. australasica L. is a potential source of bioactive compounds and could result in a positive outcome for the food, cosmetics and pharmaceutical industries. Full article
(This article belongs to the Special Issue Analytical Techniques for the Determination of Bioactive Food Compounds)
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