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10th Anniversary of Biomedicines—Advances in Development of Human Adipose Cells

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A special issue of Biomedicines (ISSN 2227-9059). This special issue belongs to the section "Cell Biology and Pathology".

Deadline for manuscript submissions: closed (15 November 2022) | Viewed by 5612

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Special Issue Information

Dear Colleagues,

The year 2023 marks the 10th anniversary of Biomedicines, a peer-reviewed open access journal in the biomedical field. So far, Biomedicines has published more than 2700 papers from more than 17,000 authors. We appreciate each author, reviewer, and academic editor whose support has brought us to where we are today.

To celebrate this significant milestone, we aim to publish a Special Issue entitled 10th Anniversary of Biomedicines—Advances in Development of Human Adipose Cells. The hypertrophy, as well as hypotrophy, of adipose tissue depots is linked to metabolic dysfunction. Thus, a better understanding of adipose tissue development is required to counteract obesity, lipodystrophy and their associated complications, with most of what is currently known about their development based on rodent models. However, cellular and metabolic differences have been reported between human and murine adipose tissues. This Special Issue focuses on human adipose cells, aiming to collect submissions of original research articles investigating the identity of the progenitors of white, brown and beige/brite adipocytes, the characterization of their niches, as well as molecules affecting their differentiation using relevant cell models and culture conditions, recapitulating a better adipocyte biology. Review articles describing the current state of the art are also welcomed.

Dr. Christian Dani
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biomedicines is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

adipose tissue
in vitro adipose tissue models
human adipose-derived cells
human adipose stem cell models
metabolic disease modelling
obesity

Published Papers (2 papers)

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Research

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19 pages, 3138 KiB

Open AccessArticle

Comparative Study of the Osteogenic Differentiation Potential of Adipose Tissue-Derived Stromal Cells and Dedifferentiated Adipose Cells of the Same Tissue Origin under Pro and Antioxidant Conditions

by Anne Bollmann, Hans Christian Sons, Jennifer Lynn Schiefer, Paul C. Fuchs, Joachim Windolf and Christoph Viktor Suschek

Biomedicines 2022, 10(12), 3071; https://0-doi-org.brum.beds.ac.uk/10.3390/biomedicines10123071 - 29 Nov 2022

Cited by 2 | Viewed by 1543

Abstract

Adipose tissue-derived stromal cells (ASCs) play an important role in various therapeutic approaches to bone regeneration. However, such applications become challenging when the obtained cells show a functional disorder, e.g., an impaired osteogenic differentiation potential (ODP). In addition to ASCs, human adipose tissue is also a source for another cell type with therapeutic potential, the dedifferentiated fat cells (DFATs), which can be obtained from mature adipocytes. Here, we for the first time compared the ODPs of each donors ASC and DFAT obtained from the same adipose tissue sample as well as the role of oxidative stress or antioxidative catalase on their osteogenic outcome. Osteogenic potential of ASC and DFAT from nine human donors were compared in vitro. Flow cytometry, staining for calcium accumulation with alizarin red, alkaline phosphatase assay and Western blots were used over an osteogenic induction period of up to 14 days. H₂O₂ was used to induce oxidative stress and catalase was used as an antioxidative measure. We have found that ASC and DFAT cultures’ ODPs are nearly identical. If ASCs from an adipose tissue sample showed good or bad ODP, so did the corresponding DFAT cultures. The inter-individual variability of the donor ODPs was immense with a maximum factor of about 20 and correlated neither with the age nor the sex of the donors of the adipose tissue. Oxidative stress in the form of exogenously added H₂O₂ led to a significant ODP decrease in both cell types, with this ODP decrease being significantly lower in DFAT cultures than in the corresponding ASC cultures. Regardless of the individual cell culture-specific ODP, however, exogenously applied catalase led to an approx. 2.5-fold increase in osteogenesis in the ASC and DFAT cultures. Catalase appears to be a potent pro-osteogenic factor, at least in vitro. A new finding that points to innovative strategies and therapeutic approaches in bone regeneration. Furthermore, our results show that DFATs behave similarly to ASCs of the same adipose tissue sample with respect to ODPs and could therefore be a very attractive and readily available source of multipotent stem cells in bone regenerative therapies. Full article

(This article belongs to the Special Issue 10th Anniversary of Biomedicines—Advances in Development of Human Adipose Cells)

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Review

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19 pages, 2106 KiB

Open AccessFeature PaperReview

Adipose Tissue Development Relies on Coordinated Extracellular Matrix Remodeling, Angiogenesis, and Adipogenesis

by Elizabeth K. Johnston and Rosalyn D. Abbott

Biomedicines 2022, 10(9), 2227; https://0-doi-org.brum.beds.ac.uk/10.3390/biomedicines10092227 - 08 Sep 2022

Cited by 6 | Viewed by 3576

Abstract

Despite developing prenatally, the adipose tissue is unique in its ability to undergo drastic growth even after reaching its mature size. This development and subsequent maintenance rely on the proper coordination between the vascular niche and the adipose compartment. In this review, the process of adipose tissue development is broken down to explain (1) the ultrastructural matrix remodeling that is undertaken during simultaneous adipogenesis and angiogenesis, (2) the paracrine crosstalk involved during adipose development, (3) the mechanical regulators involved in adipose growth, and (4) the proteolytic and paracrine oversight for matrix remodeling during adipose development. It is crucial to gain a better understanding of the complex relationships that exist between adipose tissue and the vasculature during tissue development to provide insights into the pathological tissue expansion of obesity and to develop improved soft-tissue reconstruction techniques. Full article

(This article belongs to the Special Issue 10th Anniversary of Biomedicines—Advances in Development of Human Adipose Cells)

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