Research

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10 pages, 2224 KiB

Open AccessArticle

Combinational Growth Factor and Gas Delivery for Thrombosis Prevention

by Huan Cao, Xuejuan Xu, Fuyu Zhu and Yanhui Sheng

Biomolecules 2022, 12(11), 1715; https://0-doi-org.brum.beds.ac.uk/10.3390/biom12111715 - 19 Nov 2022

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Cardiovascular stents enable the rapid re-endothelialization of endothelial cells (ECs), and the constant suppression of smooth muscle cell (SMC) proliferation has been proved to effectively prevent thrombosis. However, the development and application of such stents are still insufficient due the delayed re-endothelialization progress, [...] Read more.

Cardiovascular stents enable the rapid re-endothelialization of endothelial cells (ECs), and the constant suppression of smooth muscle cell (SMC) proliferation has been proved to effectively prevent thrombosis. However, the development and application of such stents are still insufficient due the delayed re-endothelialization progress, as well as the poor durability of the SMC inhibition. In this paper, we developed a mussel-inspired coating with the ability for the dual delivery of both growth factor (e.g., platelet-derived growth factor, PDGF) and therapeutic gas (e.g., nitric oxide, NO) for thrombosis prevention. We firstly synthesized the mussel-inspired co-polymer (DMHM) of dopamine methacrylamide (DMA) and hydroxyethyl methacrylate (HEMA) and then coated the DMHM on 316L SS stents combined with Cu^II. Afterwards, we immobilized the PDGF on the DMHM-coated stent and found that the PDGF could be released in the first 3 days to enhance the recruitment, proliferation, and migration of human umbilical vein endothelial cells (HUVECs) to promote re-endothelialization. The Cu^II could be “sealed” in the DMHM coating, with extended durability (2 months), with the capacity for catalyzed NO generation for up to 2 months to suppress the proliferation of SMCs. Such a stent surface modification strategy could enhance the development of the cardiovascular stents for thrombosis prevention. Full article

(This article belongs to the Special Issue Tissue Engineering Approaches for Reconstructing Biological Environment)

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13 pages, 3271 KiB

Open AccessArticle

Precise Design of Alginate Hydrogels Crosslinked with Microgels for Diabetic Wound Healing

by Yishu Yan, Panpan Ren, Qingqing Wu and Tianmeng Zhang

Biomolecules 2022, 12(11), 1582; https://0-doi-org.brum.beds.ac.uk/10.3390/biom12111582 - 28 Oct 2022

Cited by 3 | Viewed by 1522

Abstract

Alginate hydrogel has received great attention in diabetic wound healing. However, the limited tunability of the ionic crosslinking method prevents the delicate management of physical properties in response to diverse wound conditions. We addressed this issue by using a microgel particle (fabricated by [...] Read more.

Alginate hydrogel has received great attention in diabetic wound healing. However, the limited tunability of the ionic crosslinking method prevents the delicate management of physical properties in response to diverse wound conditions. We addressed this issue by using a microgel particle (fabricated by zinc ions and coordinated through the complex of carboxymethyl chitosan and aldehyde hyaluronic acid) as a novel crosslinker. Then the cation was introduced as a second crosslinker to create a double crosslinked network. The method leads to the precise regulation of the hydrogel characters, including the biodegradation rate and the controlled release rate of the drug. As a result, the optimized hydrogels facilitated the live-cell infiltration in vitro and boosted the tissue regeneration of diabetic wounds in vivo. The results indicated that the addition of the microgel as a new crosslinker created flexibility during the construction of the alginate hydrogel, adapting for diverse applications during diabetic-induced wound therapy. Full article

(This article belongs to the Special Issue Tissue Engineering Approaches for Reconstructing Biological Environment)

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Review

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28 pages, 5617 KiB

Open AccessReview

In Vitro Models of Ovarian Cancer: Bridging the Gap between Pathophysiology and Mechanistic Models

by Elliot Lopez, Sahil Kamboj, Changchong Chen, Zixu Wang, Sabrina Kellouche, Johanne Leroy-Dudal, Franck Carreiras, Ambroise Lambert and Carole Aimé

Biomolecules 2023, 13(1), 103; https://0-doi-org.brum.beds.ac.uk/10.3390/biom13010103 - 04 Jan 2023

Cited by 4 | Viewed by 2352

Abstract

Ovarian cancer (OC) is a disease of major concern with a survival rate of about 40% at five years. This is attributed to the lack of visible and reliable symptoms during the onset of the disease, which leads over 80% of patients to [...] Read more.

Ovarian cancer (OC) is a disease of major concern with a survival rate of about 40% at five years. This is attributed to the lack of visible and reliable symptoms during the onset of the disease, which leads over 80% of patients to be diagnosed at advanced stages. This implies that metastatic activity has advanced to the peritoneal cavity. It is associated with both genetic and phenotypic heterogeneity, which considerably increase the risks of relapse and reduce the survival rate. To understand ovarian cancer pathophysiology and strengthen the ability for drug screening, further development of relevant in vitro models that recapitulate the complexity of OC microenvironment and dynamics of OC cell population is required. In this line, the recent advances of tridimensional (3D) cell culture and microfluidics have allowed the development of highly innovative models that could bridge the gap between pathophysiology and mechanistic models for clinical research. This review first describes the pathophysiology of OC before detailing the engineering strategies developed to recapitulate those main biological features. Full article

(This article belongs to the Special Issue Tissue Engineering Approaches for Reconstructing Biological Environment)

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