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Biosensors
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Cell Based Biosensors
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Cell Based Biosensors

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor and Bioelectronic Devices".

Deadline for manuscript submissions: closed (28 February 2022) | Viewed by 12885

Special Issue Editor

Dr. Youngeun Kwon

E-Mail Website
Guest Editor
Department of Biomedical Engineering, College of Bio System, Dongguk University, Seoul 04620, Korea
Interests: biosensors and biochips; protein engineering; nanomedicine

Special Issue Information

Dear Colleagues,

Biosensing technology is one of the fastest growing fields with wide applications. While there are various types of biosensors available, cell-based sensors offer unique advantages over other in vitro biosensors. They allow the monitoring of biological targets in their native environment, where diverse biological molecules co-exist, thus providing more biologically reliable information. Cell-based sensors also enable the functional identification of unknown materials with promising biological activities. Cell-based sensors are up-and-coming tools in the biosensing field, and they hold strong potential especially in the areas of pharmacology, toxicology, as well as environmental research. It will be very interesting and beneficial to the scientific society to discuss the recent development and improvement of these sensors.

Dr. Youngeun Kwon
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • biosensors
  • cell-based sensors
  • real-time monitoring
  • functional screening
  • genetically encoded biosensors
  • environmental analysis
  • drug screening

Published Papers (4 papers)

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Research

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15 pages, 1911 KiB  
Article
A HepG2 Cell-Based Biosensor That Uses Stainless Steel Electrodes for Hepatotoxin Detection
by Martin Rozman, Zala Štukovnik, Ajda Sušnik, Amirhossein Pakseresht, Matej Hočevar, Damjana Drobne and Urban Bren
Biosensors 2022, 12(3), 160; https://0-doi-org.brum.beds.ac.uk/10.3390/bios12030160 - 04 Mar 2022
Cited by 6 | Viewed by 3081
Abstract
Humans are frequently exposed to environmental hepatotoxins, which can lead to liver failure. Biosensors may be the best candidate for the detection of hepatotoxins because of their high sensitivity and specificity, convenience, time-saving, low cost, and extremely low detection limit. To investigate suitability [...] Read more.
Humans are frequently exposed to environmental hepatotoxins, which can lead to liver failure. Biosensors may be the best candidate for the detection of hepatotoxins because of their high sensitivity and specificity, convenience, time-saving, low cost, and extremely low detection limit. To investigate suitability of HepG2 cells for biosensor use, different methods of adhesion on stainless steel surfaces were investigated, with three groups of experiments performed in vitro. Cytotoxicity assays, which include the resazurin assay, the neutral red assay (NR), and the Coomassie Brilliant Blue (CBB) assay, were used to determine the viability of HepG2 cells exposed to various concentrations of aflatoxin B1 (AFB1) and isoniazid (INH) in parallel. The viability of the HepG2 cells on the stainless steel surface was quantitatively and qualitatively examined with different microscopy techniques. A simple cell-based electrochemical biosensor was developed by evaluating the viability of the HepG2 cells on the stainless steel surface when exposed to various concentrations of AFB1 and INH by using electrochemical impedance spectroscopy (EIS). The results showed that HepG2 cells can adhere to the metal surface and could be used as part of the biosensor to determine simple hepatotoxic samples. Full article
(This article belongs to the Special Issue Cell Based Biosensors)
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11 pages, 2811 KiB  
Article
Use of PEDOT:PSS/Graphene/Nafion Composite in Biosensors Based on Acetic Acid Bacteria
by Yulia Plekhanova, Sergei Tarasov and Anatoly Reshetilov
Biosensors 2021, 11(9), 332; https://0-doi-org.brum.beds.ac.uk/10.3390/bios11090332 - 13 Sep 2021
Cited by 12 | Viewed by 2872
Abstract
Immobilization of the biocomponent is one of the most important stages in the development of microbial biosensors. In this study, we examined the electrochemical properties of a novel PEDOT:PSS/graphene/Nafion composite used to immobilize Gluconobacter oxydans bacterial cells on the surface of a graphite [...] Read more.
Immobilization of the biocomponent is one of the most important stages in the development of microbial biosensors. In this study, we examined the electrochemical properties of a novel PEDOT:PSS/graphene/Nafion composite used to immobilize Gluconobacter oxydans bacterial cells on the surface of a graphite screen-printed electrode. Bioelectrode responses to glucose in the presence of a redox mediator 2,6-dichlorophenolindophenol were studied. The presence of graphene in the composite reduced the negative effect of PEDOT:PSS on cells and improved its conductivity. The use of Nafion enabled maintaining the activity of acetic acid bacteria at the original level for 120 days. The sensitivity of the bioelectrode based on G. oxydans/PEDOT:PSS/graphene/Nafion composite was shown to be 22 μA × mM−1 × cm−2 within the linear range of glucose concentrations. The developed composite can be used both in designing bioelectrochemical microbial devices and in biotechnology productions for long-term immobilization of microorganisms. Full article
(This article belongs to the Special Issue Cell Based Biosensors)
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11 pages, 3446 KiB  
Article
Effective Cryopreservation of a Bioluminescent Auxotrophic Escherichia coli-Based Amino Acid Array to Enable Long-Term Ready-to-Use Applications
by Hee Tae Ahn, In Seung Jang, Thinh Viet Dang, Yi Hyang Kim, Dong Hoon Lee, Hyeun Seok Choi, Byung Jo Yu and Moon Il Kim
Biosensors 2021, 11(8), 252; https://0-doi-org.brum.beds.ac.uk/10.3390/bios11080252 - 26 Jul 2021
Cited by 3 | Viewed by 2534
Abstract
Amino acid arrays comprising bioluminescent amino acid auxotrophic Escherichia coli are effective systems to quantitatively determine multiple amino acids. However, there is a need to develop a method for convenient long-term preservation of the array to enable its practical applications. Here, we reported [...] Read more.
Amino acid arrays comprising bioluminescent amino acid auxotrophic Escherichia coli are effective systems to quantitatively determine multiple amino acids. However, there is a need to develop a method for convenient long-term preservation of the array to enable its practical applications. Here, we reported a potential strategy to efficiently maintain cell viability within the portable array. The method involves immobilization of cells within agarose gel supplemented with an appropriate cryoprotectant in individual wells of a 96-well plate, followed by storage under freezing conditions. Six cryoprotectants, namely dimethyl sulfoxide, glycerol, ethylene glycol, polyethylene glycol, sucrose, and trehalose, were tested in the methionine (Met) auxotroph-based array. Carbohydrate-type cryoprotectants (glycerol, sucrose, and trehalose) efficiently preserved the linearity of determination of Met concentration. In particular, the array with 5% trehalose exhibited the best performance. The Met array with 5% trehalose could determine Met concentration with high linearity (R2 value = approximately 0.99) even after storage at −20 °C for up to 3 months. The clinical utilities of the Met and Leu array, preserved at −20 °C for 3 months, were also verified by successfully quantifying Met and Leu in spiked blood serum samples for the diagnosis of the corresponding metabolic diseases. This long-term preservation protocol enables the development of a ready-to-use bioluminescent E. coli-based amino acid array to quantify multiple amino acids and can replace the currently used laborious analytical methods. Full article
(This article belongs to the Special Issue Cell Based Biosensors)
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Review

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19 pages, 2201 KiB  
Review
Intein-Mediated Protein Engineering for Cell-Based Biosensors
by Chungwon Kang, Keshab Lal Shrestha, San Kwon, Seungil Park, Jinsik Kim and Youngeun Kwon
Biosensors 2022, 12(5), 283; https://0-doi-org.brum.beds.ac.uk/10.3390/bios12050283 - 28 Apr 2022
Cited by 3 | Viewed by 3084
Abstract
Cell-based sensors provide a flexible platform for screening biologically active targets and for monitoring their interactions in live cells. Their applicability extends across a vast array of biological research and clinical applications. Particularly, cell-based sensors are becoming a potent tool in drug discovery [...] Read more.
Cell-based sensors provide a flexible platform for screening biologically active targets and for monitoring their interactions in live cells. Their applicability extends across a vast array of biological research and clinical applications. Particularly, cell-based sensors are becoming a potent tool in drug discovery and cell-signaling studies by allowing function-based screening of targets in biologically relevant environments and enabling the in vivo visualization of cellular signals in real-time with an outstanding spatiotemporal resolution. In this review, we aim to provide a clear view of current cell-based sensor technologies, their limitations, and how the recent improvements were using intein-mediated protein engineering. We first discuss the characteristics of cell-based sensors and present several representative examples with a focus on their design strategies, which differentiate cell-based sensors from in vitro analytical biosensors. We then describe the application of intein-mediated protein engineering technology for cell-based sensor fabrication. Finally, we explain the characteristics of intein-mediated reactions and present examples of how the intein-mediated reactions are used to improve existing methods and develop new approaches in sensor cell fabrication to address the limitations of current technologies. Full article
(This article belongs to the Special Issue Cell Based Biosensors)
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