Fluorescent and Luminescent Biosensors: Development and Applications

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor and Bioelectronic Devices".

Deadline for manuscript submissions: closed (15 September 2021) | Viewed by 10242

Special Issue Editors


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Guest Editor
Chemical Engineering and Bioengineering, Washington State University, Pullman, WA 99164, USA
Interests: cardiac muscle biology and mechanics; protein chemistry and engineering; fluorescence technique development; biosensor for biological and cellular applications; fluorescent material developments for solar energy harvest applications; novel solar concentrator research and applications

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Guest Editor
James Watt Scholl of Engineering, University of Glasgow, Glasgow G12 8QQ, UK
Interests: plasmonics; fluorescence enhancement; surface plasmon resonance; sensors

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Guest Editor
Department of Biomedical Sciences, Macquarie University, Sydney, NSW 2109, Australia
Interests: luminescent probes; luminescence in situ hybridization (LISH); Lanthanide immunodetection; immunoluminescent biosensors; upconversion nanoparticles biosensors

Special Issue Information

Dear Colleagues,

Fluorescent and luminescent biosensors are a highly active and interdisciplinary research field. The field has attracted much attention because of its potential to lead to low-cost, rapid, sensitive, and miniaturized bioanalytical devices. In past decades, we have witnessed a tremendous progress in translations of fluorescent and luminescent biosensors from an academic area of research into a most powerful bioanalytical and diagnostic sensor technology. Especially in recent years, this technology development/transition has been reflected in various research efforts to exploit a variety of miniaturized and, in some cases, multiplex analytical formats, such as microfluidics, microarrays, and paper-based analytical devices for quantitative analysis of a whole spectrum of individual ions, small molecules, peptides, proteins, nucleic oligoes, cells, and cellular vesicles. These progresses make fluorescent and luminescent biosensors indispensable tools in the areas from environmental monitoring, to food/agriculture safety, biological/biomedical assays, and medical prognosis and diagnosis. Many of these applications are characterized by their outstanding sensitivity, capable of detecting concentrations in the nano-/picomolar scales, impressive performance in real-time monitor of biological processes, and multiplex capability in diagnostic assays.

This research topic aims to collect recent developments and applications of fluorescent and luminescent sensors as well as highlight prospective directions in the field. We encourage authors to submit both original research and review articles on, but not limited to, the following topics:

Development of fluorescent and luminescent biosensors (molecules, proteins and nanostructure) based on all aspects of molecular spectroscopic mechanisms:

  • Fluorescent/luminescent sensors;
  • Bioluminescent/chemiluminescent sensors;
  • Forster resonance energy transfer (FRET)-based fluorescent sensors;
  • Internal charge transfer (ICT)-based fluorescent sensors;
  • Aggregation-induced enhancement (AIE)-based fluorescent sensors;
  • Anisotropy-based fluorescent sensors;
  • Metal enhanced fluorescent sensors;
  • Luminescence lifetime-based sensors;

Applications of fluorescent and luminescent biosensors in biologically-related systems:

  • Biological applications;
  • Medical diagnostics;
  • Immunoassays;
  • Point-of-care (POC) diagnostics;
  • intracellular sensing;
  • wearable chemical sensors;
  • microbial sensors;
  • Food processes and agriculture;
  • Environmental monitoring.

Prof. Dr. Wen-Ji Dong
Dr. Anthony Centeno
Dr. Nima Sayyadi
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Published Papers (2 papers)

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Research

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12 pages, 2652 KiB  
Article
Portable Chemiluminescence-Based Lateral Flow Assay Platform for the Detection of Cortisol in Human Serum
by Hyun Tae Kim, Enjian Jin and Min-Ho Lee
Biosensors 2021, 11(6), 191; https://0-doi-org.brum.beds.ac.uk/10.3390/bios11060191 - 10 Jun 2021
Cited by 18 | Viewed by 4650
Abstract
In this study, we developed the portable chemiluminescence (CL)-based lateral flow assay (LFA) platform for the detection of cortisol in human serum. Cortisol is well-known as a stress hormone due to its high relevancy for human mental and physical health, such as hypertension [...] Read more.
In this study, we developed the portable chemiluminescence (CL)-based lateral flow assay (LFA) platform for the detection of cortisol in human serum. Cortisol is well-known as a stress hormone due to its high relevancy for human mental and physical health, such as hypertension or depression. To date, a number of optical devices have provided the sensitive determination of levels of analytes. However, this modality type still requires costly optical modules. The developed CL platform is simply composed of two detection modules along with a loading part for the LFA strip. The LFA membrane contains gold nanoparticle probes conjugated with antibodies against cortisol and horseradish peroxidase (HRP), which can also efficiently increase the luminescent signal by providing many areas for anti-cortisol antibody and HRP. The measured voltage signals coming from the photodiode in a CL reader were compared with a standard microplate reader for the evaluation of accuracy. The linear range observed for cortisol was measured to be 0.78–12.5 μg/dL (R2 = 0.99) with a limit of detection (LOD) of 0.342 μg/dL. In addition, the CL-LFA reader showed a high correlation (R2 = 0.96) with the standard cortisol console (COBAS 8000, Roche), suggesting that our developed CL-based LFA platform can be usable in situ. Full article
(This article belongs to the Special Issue Fluorescent and Luminescent Biosensors: Development and Applications)
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Review

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37 pages, 4179 KiB  
Review
Exosomes as Powerful Engines in Cancer: Isolation, Characterization and Detection Techniques
by Marwa Gamal Saad, Haluk Beyenal and Wen-Ji Dong
Biosensors 2021, 11(12), 518; https://0-doi-org.brum.beds.ac.uk/10.3390/bios11120518 - 16 Dec 2021
Cited by 23 | Viewed by 4815
Abstract
Exosomes, powerful extracellular nanovesicles released from almost all types of living cells, are considered the communication engines (messengers) that control and reprogram physiological pathways inside target cells within a community or between different communities. The cell-like structure of these extracellular vesicles provides a [...] Read more.
Exosomes, powerful extracellular nanovesicles released from almost all types of living cells, are considered the communication engines (messengers) that control and reprogram physiological pathways inside target cells within a community or between different communities. The cell-like structure of these extracellular vesicles provides a protective environment for their proteins and DNA/RNA cargos, which serve as biomarkers for many malicious diseases, including infectious diseases and cancers. Cancer-derived exosomes control cancer metastasis, prognosis, and development. In addition to the unique structure of exosomes, their nanometer size and tendency of interacting with cells makes them a viable novel drug delivery solution. In recent years, numerous research efforts have been made to quantify and characterize disease-derived exosomes for diagnosis, monitoring, and therapeutic purposes. This review aims to (1) relate exosome biomarkers to their origins, (2) focus on current isolation and detection methods, (3) discuss and evaluate the proposed technologies deriving from exosome research for cancer treatment, and (4) form a conclusion about the prospects of the current exosome research. Full article
(This article belongs to the Special Issue Fluorescent and Luminescent Biosensors: Development and Applications)
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