Special Issue "Label and Label-Free Aptasensors"

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor and Bioelectronic Devices".

Deadline for manuscript submissions: 15 November 2021.

Special Issue Editors

Dr. Yugender Goud Kotagiri
E-Mail Website
Guest Editor
Nanoengineering Department, University of California San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA
Interests: biosensors; electrochemical sensor; wearable sensors; aptamers; fuel cells; electrochemistry
Special Issues, Collections and Topics in MDPI journals
Dr. Kuldeep Mahato
E-Mail Website
Guest Editor
Department of Nanoengineering, University of California San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA
Interests: biosensors; electrochemical sensor; wearable sensors; bio-fuel cells; aptamer-based sensors
Special Issues, Collections and Topics in MDPI journals
Prof. Dr. K. V. Gobi
E-Mail Website
Guest Editor
Department of Chemistry, National Institute of Technology Warangal, Warangal 506004, India
Interests: electrochemical biosensors; biomarkers; wearable sensors; aptasensors; hybrid nanomaterials; fuel cells

Special Issue Information

Dear Colleagues,

Aptamers are effectively replacing traditional antibodies in biosensors by offering comparable sensitive recognitions, thus being declared 'chemical antibodies'. These small-sized oligonucleotides offer numerous properties, including quick chemical production, versatile chemical modifications, structural flexibility, high chemical and thermal stability, etc. These 'chemical antibodies' have been employed to create accurate and sensitive diagnostic tools for amplifying the signal in various techniques and formats viz. fluorometric, colorimetric, chromatographic, lateral flow assays, and electrochemical sensing. Depending on the mechanism of sensing, aptamer-based sensors are classified under label and label-free formats. The labels can either be radioactive, fluorescent-active, electro-active molecules, metallic complex, nanoparticles, enzyme, or antibodies with detectable probes, which assist in detecting target analytes from the real samples. In the case of label-free aptasensors, the aptamer is directly incorporated onto the transducer surface, which generates the analytical signal against its physiochemical interaction with the target analyte. Thus, the generated signal is measured in correlation to the analyte’s concentrations, whereas in the labeled apatsensing formats, the signals measured are usually generated from notable changes in the labelled/tagged moieties. Aptamer-based sensors have played an important role in various fields, such as environmental, agriculture, biomedical, forensic, and security applications with highly selective, sensitive, and accurate detection of target analytes. In addition, aptamers have also been used in theranostics approaches for diagnosing various pathophysiological complications. These aptamer-based theranostics offer immediate translational ex vivo/in vivo diagnostic/theranostic applications. 

All these advanced strategies using aptamer-based techniques/platforms fall under the scope of the journal Biosensors. Thus, this Special Volume entitled 'Label and Label-free Aptasensors' will have a great impact on a wide range of readers, from industry and academia. For this issue, we would like to invite manuscripts related to label and label-free aptamer-based sensors for various target analyte detections.

Dr. Yugender Goud Kotagiri
Dr. Kuldeep Mahato
Prof. Dr. K. V. Gobi
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1800 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • biosensors
  • aptamers
  • labelled aptamers 
  • label-free aptamers
  • biomarkers
  • electrochemical sensors
  • optical sensors
  • colorimetric sensors
  • methylene-blue
  • quantum dots
  • aptamer antibody
  • aptamer enzyme
  • dual sandwich bio-recognition elements
  • aptamer-based theranostics
  • chemiluminescence sensors 

Published Papers (1 paper)

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Research

Article
Highly Sensitive Electrochemical Aptasensor for Detecting the VEGF165 Tumor Marker with PANI/CNT Nanocomposites
Biosensors 2021, 11(4), 114; https://0-doi-org.brum.beds.ac.uk/10.3390/bios11040114 - 09 Apr 2021
Cited by 1 | Viewed by 701
Abstract
Sensing targeted tumor markers with high sensitivity provides vital information for the fast diagnosis and treatment of cancer patients. A vascular endothelial growth factor (VEGF165) have recently emerged as a promising biomarker of tumor cells. The electrochemical aptasensor is a promising [...] Read more.
Sensing targeted tumor markers with high sensitivity provides vital information for the fast diagnosis and treatment of cancer patients. A vascular endothelial growth factor (VEGF165) have recently emerged as a promising biomarker of tumor cells. The electrochemical aptasensor is a promising tool for detecting VEGF165 because of its advantages such as a low cost and quantitative analysis. To produce a sensitive and stable sensor electrode, nanocomposites based on polyaniline (PANI) and carbon nanotube (CNT) have potential, as they provide for easy fabrication, simple synthesis, have a large surface area, and are suitable in biological environments. Here, a label-free electrochemical aptasensor based on nanocomposites of CNT and PANI was prepared for detecting VEGF165 as a tumor marker. The nanocomposite was assembled with immobilized VEGF165 aptamer as a highly sensitive VEGF165 sensor. It exhibited stable and wide linear detection ranges from 0.5 pg/mL to 1 μg/mL, with a limit of detection of 0.4 pg/mL because of the complementary effect of PANI/CNT. The fabricated aptasensor also exhibited good stability in biological conditions, selectivity, and reproducibility after several measurement times after the dissociation process. Thus, it could be applied for the non-invasive determination of VEGF, in biological fluid diagnosis kits, or in an aptamer-based biosensor platform in the near future. Full article
(This article belongs to the Special Issue Label and Label-Free Aptasensors)
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