Printed Electrochemical Biosensors

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor and Bioelectronic Devices".

Deadline for manuscript submissions: closed (20 December 2023) | Viewed by 9089

Special Issue Editor


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Guest Editor
Department of Chemistry, National and Kapodistrian University of Athens, 11527 Athens, Greece
Interests: low-cost sensors; 3D printing; microengineering; biosensors; immunosensors; trace metal analysis; electrochemistry; quantum dots; nanoparticles
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Special Issue Information

Dear Colleagues,

I would like to invite you to participate in a Special Issue on “Printed Electrochemical Biosensors”. Today, a key challenge in the research community is the development of smart analytical (bio)devices which can monitor significant analytes and biomarkers. The adoption of printing technologies by the research field of electrochemical sensors has gained high levels of interest as they provide the ability to produce customized devices and sensing systems that previously required more complicated and specialized procedures to manufacture.

This Special Issue aims to highlight advances in the fabrication, modification, and analytical usefulness of printed electrochemical biosensors.

Contributions to this Special Issue may include but are not limited to:

  • Electrochemical sensors produced or modified via printing technologies, such as 3D printing, screenprinting, plotter printing, wax printing, etc.;
  • Application of printed sensors in electrochemical biosensing.

Prof. Dr. Christos Kokkinos
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • 3D printing
  • screen-printing
  • plotter-printing
  • wax-printing
  • biosensors
  • electrochemistry

Published Papers (4 papers)

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Research

13 pages, 2676 KiB  
Article
Fe3O4@Au Core–Shell Magnetic Nanoparticles for the Rapid Analysis of E. coli O157:H7 in an Electrochemical Immunoassay
by Shayesteh Bazsefidpar, Maria Freitas, Clara R. Pereira, Gemma Gutiérrez, Esther Serrano-Pertierra, Henri P. A. Nouws, María Matos, Cristina Delerue-Matos and María Carmen Blanco-López
Biosensors 2023, 13(5), 567; https://0-doi-org.brum.beds.ac.uk/10.3390/bios13050567 - 22 May 2023
Cited by 2 | Viewed by 1414
Abstract
Escherichia coli (E. coli) O157:H7 is a pathogenic bacterium that causes serious toxic effects in the human gastrointestinal tract. In this paper, a method for its effective analytical control in a milk sample was developed. To perform rapid (1 h) and [...] Read more.
Escherichia coli (E. coli) O157:H7 is a pathogenic bacterium that causes serious toxic effects in the human gastrointestinal tract. In this paper, a method for its effective analytical control in a milk sample was developed. To perform rapid (1 h) and accurate analysis, monodisperse Fe3O4@Au magnetic nanoparticles were synthesized and used in an electrochemical sandwich-type magnetic immunoassay. Screen-printed carbon electrodes (SPCE) were used as transducers, and electrochemical detection was performed by chronoamperometry using a secondary horseradish peroxidase-labeled antibody and 3,3′,5,5′-tetramethylbenzidine. This magnetic assay was used to determine the E. coli O157:H7 strain in the linear range from 20 to 2 × 106 CFU/mL, with a limit of detection of 20 CFU/mL. The selectivity of the assay was tested using Listeria monocytogenes p60 protein, and the applicability of the assay was assessed by analyzing a commercial milk sample, demonstrating the usefulness of the synthesized nanoparticles in the developed magnetic immunoassay. Full article
(This article belongs to the Special Issue Printed Electrochemical Biosensors)
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13 pages, 2285 KiB  
Article
3D Printed Voltammetric Sensor Modified with an Fe(III)-Cluster for the Enzyme-Free Determination of Glucose in Sweat
by Eleni Koukouviti, Alexios K. Plessas, Anastasios Economou, Nikolaos Thomaidis, Giannis S. Papaefstathiou and Christos Kokkinos
Biosensors 2022, 12(12), 1156; https://0-doi-org.brum.beds.ac.uk/10.3390/bios12121156 - 11 Dec 2022
Cited by 3 | Viewed by 1750
Abstract
In this work, a 3D printed sensor modified with a water-stable complex of Fe(III) basic benzoate is presented for the voltammetric detection of glucose (GLU) in acidic epidermal skin conditions. The GLU sensor was produced by the drop-casting of Fe(III)-cluster ethanolic mixture on [...] Read more.
In this work, a 3D printed sensor modified with a water-stable complex of Fe(III) basic benzoate is presented for the voltammetric detection of glucose (GLU) in acidic epidermal skin conditions. The GLU sensor was produced by the drop-casting of Fe(III)-cluster ethanolic mixture on the surface of a 3D printed electrode fabricated by a carbon black loaded polylactic acid filament. The oxidation of GLU was electrocatalyzed by Fe(III), which was electrochemically generated in-situ by the Fe(III)-cluster precursor. The GLU determination was carried out by differential pulse voltammetry without the interference from common electroactive metabolites presented in sweat (such as urea, uric acid, and lactic acid), offering a limit of detection of 4.3 μmol L−1. The exceptional electrochemical performance of [Fe3O(PhCO2)6(H2O)3]∙PhCO2 combined with 3D printing technology forms an innovative and low-cost enzyme-free sensor suitable for noninvasive applications, opening the way for integrated 3D printed wearable biodevices. Full article
(This article belongs to the Special Issue Printed Electrochemical Biosensors)
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20 pages, 18879 KiB  
Article
Electrochemical Biosensor for SARS-CoV-2 cDNA Detection Using AuPs-Modified 3D-Printed Graphene Electrodes
by Luiz R. G. Silva, Jéssica S. Stefano, Luiz O. Orzari, Laís C. Brazaca, Emanuel Carrilho, Luiz H. Marcolino-Junior, Marcio F. Bergamini, Rodrigo A. A. Munoz and Bruno C. Janegitz
Biosensors 2022, 12(8), 622; https://0-doi-org.brum.beds.ac.uk/10.3390/bios12080622 - 10 Aug 2022
Cited by 25 | Viewed by 2766
Abstract
A low-cost and disposable graphene polylactic (G-PLA) 3D-printed electrode modified with gold particles (AuPs) was explored to detect the cDNA of SARS-CoV-2 and creatinine, a potential biomarker for COVID-19. For that, a simple, non-enzymatic electrochemical sensor, based on a Au-modified G-PLA platform was [...] Read more.
A low-cost and disposable graphene polylactic (G-PLA) 3D-printed electrode modified with gold particles (AuPs) was explored to detect the cDNA of SARS-CoV-2 and creatinine, a potential biomarker for COVID-19. For that, a simple, non-enzymatic electrochemical sensor, based on a Au-modified G-PLA platform was applied. The AuPs deposited on the electrode were involved in a complexation reaction with creatinine, resulting in a decrease in the analytical response, and thus providing a fast and simple electroanalytical device. Physicochemical characterizations were performed by SEM, EIS, FTIR, and cyclic voltammetry. Square wave voltammetry was employed for the creatinine detection, and the sensor presented a linear response with a detection limit of 0.016 mmol L−1. Finally, a biosensor for the detection of SARS-CoV-2 was developed based on the immobilization of a capture sequence of the viral cDNA upon the Au-modified 3D-printed electrode. The concentration, immobilization time, and hybridization time were evaluated in presence of the DNA target, resulting in a biosensor with rapid and low-cost analysis, capable of sensing the cDNA of the virus with a good limit of detection (0.30 µmol L−1), and high sensitivity (0.583 µA µmol−1 L). Reproducible results were obtained (RSD = 1.14%, n = 3), attesting to the potentiality of 3D-printed platforms for the production of biosensors. Full article
(This article belongs to the Special Issue Printed Electrochemical Biosensors)
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8 pages, 1336 KiB  
Communication
Portable Vertical Graphene@Au-Based Electrochemical Aptasensing Platform for Point-of-Care Testing of Tau Protein in the Blood
by Yibiao Liu, Xingyun Liu, Mifang Li, Qiong Liu and Tailin Xu
Biosensors 2022, 12(8), 564; https://0-doi-org.brum.beds.ac.uk/10.3390/bios12080564 - 25 Jul 2022
Cited by 11 | Viewed by 2002
Abstract
Alzheimer’s disease (AD) is a long-term neurodegenerative disease that poses a serious threat to human life and health. It is very important to develop a portable quantitative device for AD diagnosis and personal healthcare. Herein, we develop a portable electrochemical sensing platform for [...] Read more.
Alzheimer’s disease (AD) is a long-term neurodegenerative disease that poses a serious threat to human life and health. It is very important to develop a portable quantitative device for AD diagnosis and personal healthcare. Herein, we develop a portable electrochemical sensing platform for the point-of-care detection of AD biomarkers in the blood. Such a portable platform integrates nanoAu-modified vertical graphene (VG@Au) into a working electrode, which can significantly improve sensitivity and reduce detection limit due to the large specific surface, excellent electrical conductivity, high stability, and good biocompatibility. The tau protein, as an important factor in the course of AD, is selected as a key AD biomarker. The results show that the linear range of this sensing platform is 0.1 pg/mL to 1 ng/mL, with a detection limit of 0.034 pg/mL (S/N = 3), indicating that this portable sensing platform meets the demand for the detection of the tau protein in the blood. This work offers great potential for AD diagnosis and personal healthcare. Full article
(This article belongs to the Special Issue Printed Electrochemical Biosensors)
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