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Dentistry Journal
Special Issues
Molecular Diagnostics in Oral Diseases
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Molecular Diagnostics in Oral Diseases

A special issue of Dentistry Journal (ISSN 2304-6767). This special issue belongs to the section "Oral Hygiene, Periodontology and Peri-implant Diseases".

Deadline for manuscript submissions: closed (1 August 2022) | Viewed by 11182

Special Issue Editors

Prof. Dr. Marinka Mravak-Stipetić

E-Mail Website
Guest Editor
Faculty of Dental Medicine and Health, University Josip Juraj Strossmayer Osijek, HR-31000 Osijek, Croatia
Interests: molecular diagnostics of oral diseases; oral diagnostics; oral infections; oral-systemic health connection; multidisciplinary approach to oral diseases; applications of pharmacogenomics in oral diseases; pharmacogenetic testing in oral adverse drug reactions
Special Issues, Collections and Topics in MDPI journals
Dr. Ivan Salarić

E-Mail Website
Guest Editor
Department of Oral Surgery, School of Dental Medicine, University of Zagreb, Gundulićeva 5, 10000 Zagreb, Croatia
Interests: salivary diagnostics; salivary biomarkers; oral cancer; molecular diagnostics of oral diseases; dental implantology; oral and maxillofacial pathology
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

It is a privilege to invite you to submit your paper and present your research in the upcoming Special Issue “Molecular Diagnostics in Oral Diseases“, which will be considered for publication in Dentistry Journal.

Molecular diagnostics has revolutionized diagnostics by providing research, diagnosis, and treatment of many diseases at the molecular level of DNA, RNA or proteins, contributing to the molecular characterization of various diseases. Understanding the molecular pathogenesis of oral diseases based on the application of molecular diagnostics techniques with a goal to expedite diagnosis, prognosis, prevention and monitoring of disease or creating patient-based therapy presents particular challenges in dental medicine. 

This Special Issue addresses a wide range of oral diseases and their molecular characterization based on the analysis of oral tissues and oral fluids using standard molecular genetics and -omics technologies.

The purpose of this issue is to create a platform to present new information and expand current knowledge about the molecular basis of oral diseases in order to pinpoint early variations and diagnosis, predict the course and enable personalized treatment by developing, applying and monitoring the impact of new drugs in the treatment and prevention of oral diseases.

To contribute to this rapidly developing field of oral diseases diagnostics, please refer to the keywords below to submit your research for this Special Issue.

Prof. Dr. Marinka Mravak-Stipetić
Dr. Ivan Salarić
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Dentistry Journal is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2000 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Molecular diagnostics of oral diseases
  • Molecular techniques
  • Oral microbiome
  • Salivary microbiome
  • Salivary metabolome
  • Oral infections
  • Oral and maxillofacial pathology
  • Oral cancer
  • Oral health in the era of personalized medicine
  • Salivary biomarkers
  • Genomic dentistry
  • Oral chronic cGVHD
  • Pharmacogenomics
  • Clinical research.

Published Papers (4 papers)

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Research

10 pages, 545 KiB  
Article
Salivary Scca1, Scca2 and Trop2 in Oral Cancer Patients—A Cross-Sectional Pilot Study
by Ivana Karmelić, Ivan Salarić, Ksenija Baždarić, Marko Rožman, Ivan Zajc, Marinka Mravak-Stipetić, Ivona Bago, Davor Brajdić, Jasna Lovrić and Darko Macan
Dent. J. 2022, 10(4), 70; https://0-doi-org.brum.beds.ac.uk/10.3390/dj10040070 - 15 Apr 2022
Cited by 3 | Viewed by 2382
Abstract
Oral squamous cell carcinoma (OSCC) is frequently diagnosed in the advanced stages. The purpose of this paper is to determine the salivary values of SCCA1, SCCA2 and TROP2 in patients with T1N0M0 OSCC and to compare them with the values obtained from healthy [...] Read more.
Oral squamous cell carcinoma (OSCC) is frequently diagnosed in the advanced stages. The purpose of this paper is to determine the salivary values of SCCA1, SCCA2 and TROP2 in patients with T1N0M0 OSCC and to compare them with the values obtained from healthy individuals. Unstimulated (UWS) and stimulated (SWS) saliva was sampled from 29 patients with T1N0M0 OSCC and 29 sex- and age-matched healthy individuals. Statistical difference was observed in SCCA1 and SCCA2 levels both in UWS and SWS samples. TROP2 was not measurable in most of the salivary samples. Both SCCA1 and SCCA2 could represent potential biomarkers for the early-stage OSCC. Research on a larger sample and biomarker validation is needed to assess the clinical potential of SCCA1 and SCCA2 in the OSCC early diagnostics. Full article
(This article belongs to the Special Issue Molecular Diagnostics in Oral Diseases)
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19 pages, 1943 KiB  
Article
Investigation of the Molecular Profile of Granular Cell Tumours and Schwannomas of the Oral Cavity
by Benjamin Rogala, Zia Ali Khan, Linda Jackson-Boeters and Mark Roger Darling
Dent. J. 2022, 10(3), 38; https://0-doi-org.brum.beds.ac.uk/10.3390/dj10030038 - 04 Mar 2022
Cited by 2 | Viewed by 2602
Abstract
Granular cell tumours (GCTs) are rare submucosal lesions, thought to develop from Schwann cells, characterised by large polygonal cells with abundant lysosomes. The objectives of this study are to investigate whether GCTs have an antigen-presenting cell (APC) phenotype or a neural crest phenotype [...] Read more.
Granular cell tumours (GCTs) are rare submucosal lesions, thought to develop from Schwann cells, characterised by large polygonal cells with abundant lysosomes. The objectives of this study are to investigate whether GCTs have an antigen-presenting cell (APC) phenotype or a neural crest phenotype using immunohistochemistry and to compare expression profiles with Schwannomas. Immunoreactivity to CD68, HLA-DR, CD163, CD40 and CD11c (APC phenotype) and markers of neural crest cell (NCC) origin S100, SOX10, NSE and GAP43 in 23 cases of GCTs and 10 cases of Schwannomas were evaluated. RT-qPCR was used to identify a possible NCC developmental phenotype in 6 cases of GCTs. GAP43 was identified as a new NCC marker for GCTs, and some evidence was found for an APC phenotype from CD68 and HLA-DR immunoreactivity. RT-qPCR failed to identify an NCC developmental phenotype of GCTs, likely due to technical issues. Full article
(This article belongs to the Special Issue Molecular Diagnostics in Oral Diseases)
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10 pages, 531 KiB  
Article
Subgingival Microbiota Profile in Association with Cigarette Smoking in Young Adults: A Cross-Sectional Study
by Krešimir Bašić, Kristina Peroš, Zrinka Bošnjak and Ivana Šutej
Dent. J. 2021, 9(12), 150; https://0-doi-org.brum.beds.ac.uk/10.3390/dj9120150 - 11 Dec 2021
Cited by 5 | Viewed by 2473
Abstract
While smoking is recognized as one of the factors for the development and progression of periodontal diseases, a relation between the composition of the subgingival microbiota and smoking is yet to be elucidated. The aim of this study was to investigate the prevalence [...] Read more.
While smoking is recognized as one of the factors for the development and progression of periodontal diseases, a relation between the composition of the subgingival microbiota and smoking is yet to be elucidated. The aim of this study was to investigate the prevalence of subgingival bacteria in young smokers and non-smokers without clinical signs of periodontal disease. In this cross-sectional study, performed at the Department of Pharmacology, School of Dental Medicine, University of Zagreb, we enrolled 32 periodontally healthy smokers and 32 non-smokers, aged 25–35 years old. The number of oral bacteria and the prevalence of particular bacteria were assessed for each subject. Subgingival plaque samples were collected with sterile paper points from two first molars for microbiological analyses with MALDI-TOF mass spectrometry. In smokers, a significantly higher prevalence of Actinomyces odontolyticus was observed compared to non-smokers, and a significantly lower prevalence of Streptococcus sanguinis was observed compared to non-smokers. Smoking affects the composition of subgingival microbiota, either via depletion of beneficial bacteria or the increase in pathogenic bacteria. Full article
(This article belongs to the Special Issue Molecular Diagnostics in Oral Diseases)
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10 pages, 710 KiB  
Article
Molecular Screening and Analysis Reveal Novel Oral Site-Specific Locations for the Cariogenic Pathogen Scardovia wiggsiae
by Steven McDaniel, Jaydene McDaniel, Katherine M. Howard and Karl Kingsley
Dent. J. 2021, 9(6), 73; https://0-doi-org.brum.beds.ac.uk/10.3390/dj9060073 - 17 Jun 2021
Cited by 10 | Viewed by 2681
Abstract
Introduction: Scardovia wiggsiae (SW) is a newly identified cariogenic pathogen associated with severe early childhood caries and oral disease. New studies have confirmed the presence of this organism among clinical samples from both pediatric and adult patients. However, the recent discovery of this [...] Read more.
Introduction: Scardovia wiggsiae (SW) is a newly identified cariogenic pathogen associated with severe early childhood caries and oral disease. New studies have confirmed the presence of this organism among clinical samples from both pediatric and adult patients. However, the recent discovery of this organism has left researchers with only limited information available regarding the prevalence of this organism—and virtually no information regarding oral site-specific locations. Based upon this lack of information, the overall objective of this study was to perform an oral site-specific analysis of SW prevalence from clinical samples. Methods: Using an approved human subjects protocol, samples (n = 60) from an existing saliva and site-specific biorepository were identified and screened for SW presence using quantitative polymerase chain reaction (qPCR). These data were summarized and subsequently analyzed for correlations with demographic (age, sex, race or ethnicity) or clinical (body mass index or BMI, primary/mixed/permanent dentition, orthodontic brackets) variables. Results: These data revealed that average DNA concentrations from all sample sites (saliva, dorsum of tongue, gingival crevicular fluid (GCF), biofilm of upper buccal molar, and biofilm of lower lingual incisor) ranged between 13.74 and 14.69 μg/μL, with an overall average of 14.30 μg/μL ± 1.12 (standard error or SE). qPCR screening revealed a total of n = 34/60 or 56.7% of patient samples harboring SW. A total of n = 71/170 specific oral sites harbored this organism, with the majority of the SW-positive participant samples harboring SW at more than one oral site, n = 22/34 or 64.7%, including non-traditional sites such as GCF and the dorsum of the tongue. Weak correlations were found between specific SW outcomes in GCF and type of dentition (permanent; R = 0.2444), as well as SW outcomes in saliva with age (R = 0.228) and presence of orthodontic brackets (R = 0.2118). Conclusions: This study may be among the first to provide oral site-specific analysis to reveal the prevalence and location of Scardovia among clinical patient samples. Moreover, these data also provide some of the first evidence to suggest this organism may be present not only in traditional supragingival tooth-associated biofilm sites, but also in non-traditional oral sites including the dorsum of the tongue and the gingival crevice. Based upon these results, these data may represent a significant advance in our understanding of the potential sites and locations that harbor this organism and may help contribute to our understanding of the prevalence, distribution and potential for the development of oral disease among clinic patients. Full article
(This article belongs to the Special Issue Molecular Diagnostics in Oral Diseases)
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