Diversity

The microbial community composition of coastal dunes can vary across environmental gradients, with the potential to impact erosion and deposition processes. In coastal foredunes, invasive plant species establishment can create and alter environmental gradients, thereby altering microbial communities and other ecogeomorphic processes with implications for storm response and management and conservation efforts. However, the mechanisms of these processes are poorly understood. To understand how changing microbial communities can alter these ecogeomorphic dynamics, one must first understand how soil microbial communities vary as a result of invasion. Towards this goal, bacterial communities were assessed spatially along foredune microhabitats, specifically in barren foredune toe and blowout microhabitats and in surrounding vegetated monocultures of native Ammophila breviligulata and invasive Carex kobomugi. Across dune microhabitats, microbial composition was more dissimilar in barren dune toe and blowout microhabitats than among the two plant species, but it did not appear that it would favor the establishment of one plant species over the other. However, the subtle differences between the microbial community composition of two species could ultimately aid in the success of the invasive species by reducing the proportions of bacterial genera associated exclusively with A. breviligulata. These results suggest that arrival time may be crucial in fostering microbiomes that would further the continued establishment and spread of either plant species. Full article

The development of photovoltaic (PV) utility-scale solar energy (USSE) in the desert Southwest has the potential to negatively affect birds through collision mortality. Based on early patterns in fatality monitoring data, the lake effect hypothesis (LEH) was developed and suggested that birds misinterpret PV solar panels for water. As the LEH was only recently defined and inference beyond bird mortality is limited, our research objective was to examine the species composition, abundance, and distribution of live and dead aquatic habitat birds at five PV solar facilities and paired reference areas in southern California. Further, we collected data from a small regional lake as an indicator of the potential aquatic habitat bird community that could occur at our study sites. Using an ordination analysis, we found the lake grouped away from the other study sites. Although the bird community (live and dead) at the solar facilities contained aquatic habitat species, Chao’s diversity was higher, and standardized use was more than an order of magnitude higher at the lake. Finally, we did not observe aquatic habitat bird fatalities in the desert/scrub and grassland reference areas. Thus, the idea of a “lake effect” in which aquatic habitat birds perceive a PV USSE facility as a waterbody and are broadly attracted is likely a nuanced process as a PV solar facility is unlikely to provide a signal of a lake to all aquatic habitat birds at all times. Full article

This study revised the subgenus Burlinius Lopatin, 1965, of the genus Cryptocephalus Geoffroy, 1762 (Coleoptera, Chrysomelidae, Cryptocephalinae, Cryptocephalini), and describes four new species, namely, Cryptocephalus (Burlinius) longchiensis sp. nov., C. (Burlinius) baowenzhengi sp. nov., C. (Burlinius) tomurensis sp. nov., and C. (Burlinius) glabrelegantulus sp. nov. The species C. (Burlinius) turpis Chen, 1942, and C. (Burlinius) yangweii Chen, 1942, were revalidated and treated as distinct species. Another four species were transferred into this subgenus from the subgenus Cryptocephalus, namely, C. (Burlinius) flavolimbatus Pic, 1920 (stat. nov.), C. (Burlinius) pusus Schöller, 2009 (stat. nov.), C. (Burlinius) scutemaculatus Tan, 1992 (stat. nov.), and C. (Burlinius) shaowuanus Gressitt & Kimoto, 1961 (stat. nov.). Two species (including one more subspecies) were removed out of this subgenus, namely, C. (Burlinius) nigrolimbatus Jacoby, 1890, C. (Burlinius) pallidipes Pic, 1927, and C. (Burlinius) pallidipes nakatae Gressitt & Kimoto, 1961; they were transferred into the subgenus Cryptocephalus. Thus, the subgenus Burlinius Lopatin includes now a total of 26 species in China according to our revision. A key to all the Chinese species of this subgenus is provided as well as color illustrations and line drawings for the general habitus and genital structures. Full article

One of the many taxonomic challenges found in the Dendropsophus microcephalus species group is the Dendropsophus walfordi distinction from D. nanus. Recent phylogenetic inferences have indicated the paraphyly of these species, although they were not designed to assess this issue. To contribute to the delimitation of these species, we analyzed the 12S, 16S and COI mitochondrial genes, the morphological traits, and the advertisement calls of specimens from northern Amazonia to Argentina, including the type localities of D. nanus and D. walfordi. Paraphyly of D. nanus with respect to D. walfordi was inferred by maximum-parsimony and Bayesian analyses, and five major clades exhibiting nonoverlapping geographic distributions were recognized. The bPTP and ABGD analyses supported the existence of five independently evolving lineages in this complex. Acoustic and morphological data clearly distinguished the clade that included the topotypes of D. walfordi from the others, corroborating the validity of this species. To avoid the paraphyly of D. nanus with respect to D. walfordi, we recognize the clade distributed from central-southern Brazil to Argentina as D. nanus, the clade distributed in Amazonia as D. walfordi, and discuss the existence of unnamed cryptic species closely related to D. nanus and D. walfordi. Full article

Parrots (Psittaciformes) are a well-studied, diverse group of birds distributed mainly in tropical and subtropical regions. Today, one-third of their species face extinction, mainly due to anthropogenic threats. Emerging tools in genetics have made major contributions to understanding basic and applied aspects of parrot biology in the wild and in captivity. In this review, we show how genetic methods have transformed the study of parrots by summarising important milestones in the advances of genetics and their implementations in research on parrots. We describe how genetics helped to further knowledge in specific research fields with a wide array of examples from the literature that address the conservation significance of (1) deeper phylogeny and historical biogeography; (2) species- and genus-level systematics and taxonomy; (3) conservation genetics and genomics; (4) behavioural ecology; (5) molecular ecology and landscape genetics; and (6) museomics and historical DNA. Finally, we highlight knowledge gaps to inform future genomic research on parrots. Our review shows that the application of genetic techniques to the study of parrot biology has far-reaching implications for addressing diverse research aims in a highly threatened and charismatic clade of birds. Full article

Six Gram-stain-negative, aerobic, rod-shaped, and motile by gliding bacterial strains were isolated from Pacific green and red algae. Phylogenetic analysis based on 16S rRNA gene sequences placed the novel strains into the genus Zobellia as a distinct evolutionary lineage close to Zobellia nedashkovskayae Asnod2-B07-B^T and Zobellia laminariae KMM 3676^T sharing the highest similarity of 99.7% and 99.5%, respectively. The average nucleotide identity and the average amino acid identity values between strains 36-CHABK-3-33^T and Z. nedashkovskayae Asnod2-B07-B^T and Z. laminariae KMM 3676^T were 89.7%/92.9% and 94.2%/95.8%, respectively. The digital DNA–DNA hybridization values based on the draft genomes between strains 36-CHABK-3-33^T and Z. nedashovskayae Asnod2-B07-B^T and Z. laminariae KMM 3676^T were 39.5 ± 2.5% and 59.6 ± 2.7%, respectively. Multilocus sequence analysis based on house-keeping genes (dnaK, gyrB, pyrH, recA and topA) assigned the alga-associated isolates to the same species, which clustered separately from the recognized species of the genus Zobellia. The strains under study grew at 4–32 °C and with 0.5–8% NaCl and decomposed aesculin, gelatin, DNA, and Tweens 20 and 80, and weakly agar. The DNA G+C content was 36.7% calculated from genome sequence analysis for the strain 36-CHABK-3-33^T. The predominant fatty acids of strain 36-CHABK-3-33^T (> 5% of the total fatty acids) were iso-C_17:0 3-OH, summed feature 3 (comprising C_16:1 ω7c and/or iso-C_15:0 2-OH fatty acids), iso-C_15:0, iso-C_15:1 G, and C_15:0. The major polar lipids were phosphatidylethanolamine, three unidentified lipids, and two unidentified aminolipids. The only detected respiratory quinone was MK-6. The significant molecular distinctiveness between the novel isolates and their nearest neighbor was strongly supported by differences in physiological and biochemical tests. Therefore, the six novel strains represent a novel species of the genus Zobellia, for which the name Zobellia barbeyronii sp. nov. is proposed. The type strain is 36-CHABK-3-33^T (= KACC 21790^T = KMM 6746^T). Full article

The construction and continued use of transportation infrastructure, specifically roads, has had a significant global impact on biodiversity and the environment. The Republic of Korea, or South Korea, has a road density of 1.13 km/km². So far, three nationwide studies about vertebrate road-killed species have been reported, showing bias towards medium to large mammals, the most common victims being the Korean water deer (Hydropotes inermis), Korean hare (Lepus coreanus), Siberian roe deer (Capreolus pygargus), and the common raccoon dog (Nyctereutes procyonoides). Road-kills, or wildlife-vehicle collisions (WVCs), tend to occur in or near preferred habitat types or in highly fragmented areas, with roads additionally being linked to habitat fragmentation and loss. Alongside WVCs and habitat effects, information about other adverse effects on biodiversity is scant, although there are reports that heavy metals and other pollutants from road runoff impact marine biodiversity, vegetation, soil, and groundwater. Furthermore, roads have been linked to a prevalence of invasive plant species. To mitigate road impacts, the South Korean government has constructed, with mixed results, 530 wildlife crossing structures, mainly including overpasses and tunnels. To mitigate road impacts more effectively, the country will need more construction, monitoring, and consistent management of wildlife crossing structures. Further, incorporating plans for wildlife crossing structures in early stages of road development will be required. Full article

The uppermost Jurassic to lowermost Cretaceous stem turtle Pleurosternon bullockii is the pleurosternid (Testudinata, Paracryptodira) known by the largest number of specimens worldwide, composing the largest European collection of Lower Cretaceous complete and partial shells for a turtle taxon. The availability of numerous specimens as well as their generally good preservation allowed for recent detailed characterization of the shell of this species, including states that are variable at the intraspecific level (individual variability, sexual dimorphism, and ontogenetic development). However, extreme cases of morphological variation corresponding to anomalies have not been addressed in detail, neither for P. bullockii nor for any other member of Paracryptodira. In this context, the study of several shell anomalies in P. bullockii is carried out here. Fourteen specimens showing anomalies are recognized and examined here to determine the frequency and distribution of these shell anatomical deviations. All these anomalies are described and figured. The morphogenetic cause of each of them is discussed. As a consequence, a relatively broad spectrum of anomalies is reported for P. bullockii. None of the anomalies seem to present negative consequences for vital activities of the specimens since none compromised the main functions of the shell. Full article

Asexual morphs of freshwater fungi have been mostly reported from tropical and subtropical regions. From our ongoing investigation of the diversity and taxonomy of freshwater microfungi in Thailand, a country with rich natural resources and diverse ecosystems, Parafuscosporella ellipsoconidiogena sp. nov. and P. obovata sp. nov., collected from decaying submerged twigs at Phalad Waterfall in a conserved forest in Chiang Mai Zoo, Chiang Mai Province, northern Thailand, are proposed. DNA phylogenies based on a combination of ITS and LSU datasets support the placement of these species in Parafuscosporella (Fuscosporellaceae, Fuscosporellales, Sordariomycetes), and these two novel species differ from known species in terms of morphology. Detailed descriptions, illustrations and a key to Parafuscosporella species are provided, as well as comparisons with other accepted Parafuscosporella species. Full article

During a survey of microfungi associated with palms from karst formations, three novel ascomycetes were found from decaying petioles of Trachycarpus fortunei (Arecaceae) in Guizhou, China. Multi-gene phylogenetic analyses based on a combined SSU, ITS, LSU, RPB2 and TEF1α sequence data showed that these collections were affiliated to Brunneofusispora and Neooccultibambusa in the family Occultibambusaceae. A new species Brunneofusispora inclinatiostiola is introduced. It is phylogenetically close to B. clematidis but represents a distinct lineage. Morphologically, it differs from the latter in having immersed ascomata with eccentric, periphysate ostiole and smaller ascospores. Morpho-phylogenetic evidence also revealed two new Neooccultibambusa species, N. kaiyangensis and N. trachycarpi. Together with the generic type N. chiangraiensis, they formed a distinct lineage within the genus Neooccultibambusa. Three novel palmicolous fungi of Occultibambusaceae are described, illustrated and notes on their identification are provided. The ecological significance of the new taxa and the phylogenetic relationship of genera in Occultibambusaceae is discussed. Full article

Reconstructing biogeographic history is challenging when dispersal biology of studied species is poorly understood, and they have undergone a complex geological past. Here, we reconstruct the origin and subsequent dispersal of coin spiders (Nephilidae: Herennia Thorell), a clade of 14 species inhabiting tropical Asia and Australasia. Specifically, we test whether the all-Asian range of Herennia multipuncta is natural vs. anthropogenic. We combine Anchored Hybrid Enrichment phylogenomic and classical marker phylogenetic data to infer species and population phylogenies. Our biogeographical analyses follow two alternative dispersal models: ballooning vs. walking. Following these assumptions and considering measured distances between geographical areas through temporal intervals, these models infer ancestral areas based on varying dispersal probabilities through geological time. We recover a wide ancestral range of Herennia including Australia, mainland SE Asia and the Philippines. Both models agree that H. multipuncta internal splits are generally too old to be influenced by humans, thereby implying its natural colonisation of Asia, but suggest quite different colonisation routes of H. multipuncta populations. The results of the ballooning model are more parsimonious as they invoke fewer chance dispersals over large distances. We speculate that coin spiders’ ancestor may have lost the ability to balloon, but that H. multipuncta regained it, thereby colonising and maintaining larger areas. Full article

Our knowledge about the status of agrobiodiversity on farms is still very limited. While several studies to assess the crop genetic diversity on farms have been undertaken, there are no systematic documentation and monitoring practices for varietal diversity in space and time. Achievement of the agrobiodiversity Aichi Target 13, established under the Biodiversity Strategy Plan 2011–2020, have failed due to the lack of existing data on varietal diversity at country, regional and global levels. Here, we propose a method for calculating the Varietal Threat Index using the four-cell analysis (FCA) participatory methodology at different geographical scales to monitor changes in the varietal diversity on farms and to compare between areas. We tested the method with datasets collected from the UN-Environment GEF project implemented in India, in which data on crop and varietal diversity were collected across seven states in India, covering five agroecological regions. Results showed that landraces are still commonly grown in the study sites, especially in the central and western regions, and that more than 50% of landraces are considered threatened, suggesting that conservation interventions are required to prevent large-scale genetic erosion. A long-term monitoring framework for varietal diversity in India is proposed. Full article

Our work shows the efficacy of DNA barcoding for recognizing the early stages of freshwater fish. We collected 3195 larvae and juveniles. Of them, we identified 43 different morphotypes. After DNA barcodes of 350 specimens, we ascertained 7 orders, 12 families, 19 genera, 20 species, and 20 Barcode Index Numbers, corresponding to putative species. For the first time, we reported the presence of the brackish species, Gobiosoma yucatanum in Lake Bacalar. Specimens of the genus Atherinella sp. and Anchoa sp. are possibly new species. Using both methods, morphology, and DNA barcodes, we identified 95% of the total larvae collected (2953 to species, and 78 to genus), and all of them were native. From them, the order Gobiiformes represented 87%. The most abundant species were Lophogobius cyprinoides and Dormitator maculatus, followed by Gobiosoma yucatanum and Ctenobius fasciatus. The Muyil and Chuyanché lagoons have the highest number of species. We present for the first time a short description of Cyprinodon artifrons and Floridichthys polyommus. This information conforms an indispensable baseline for ecological monitoring, to evaluate impacts, and developing management and conservation plans of biodiversity, principally in areas under human pressure such as Sian Ka’an, and Lake Bacalar, where tourism is high and growing in disorder. Full article

For centuries, indigenous sheep have been bred in extensive low-input systems in Midwestern Brazil. The hypothesis of this study was the assumption that phenotypic evaluation of indigenous livestock may drive the establishment of breed standards and official breed recognition, and, therefore, promote more local business opportunities. On the basis of more integrative and participatory theoretical background to applying any decision based on phenotype, we designed this research to determine the most typical and unusual phenotypes of Pantaneiro sheep. Pantaneiro ewes (281 ewes from five conservation units in five counties) were evaluated, bearing in mind both conservation and development. Descriptive statistics were used to classify ewes into typical, intermediate, and unusual phenotypes. Chi-squared tests for association were performed to test if morphological variation in the different sampling sites occurred randomly (p > 0.05) or not (p < 0.05). Some results suggest some sort of diversifying selection pressure, i.e., distinct preferences among keepers. We observed considerable morphologic variation among ewes, but it was straightforward to determine the predominant phenotypes. The study evokes integrative agrobiodiversity by putting phenotypical characterization of indigenous livestock into perspective. Discussions coming from this study may support innovative governance and participative decision-making, allied with strategies that value the socioeconomic, biocultural, and adaptive aspects of indigenous livestock in tropical regions and developing countries. This is a challenge for government, rare-breed keepers, value-chain actors, and civil society. Full article

Studies on applying of soil management practices in the management of paricá and the effects on growth and yield are essential to auxiliary its cultivation and would allow us to inform management and conservation decisions to reconciliate biodiversity, wellbeing, and sustainable production. This case study aimed to evaluate the growth and yield of paricá at different soil management practices, including consortium with an agricultural production, in the Brazilian Amazon. Paricá was implanted, consorted with soybean in the first year, and maize in the second, in a 5 × 2 m spacing. The treatments T1 = subsoiling, basal dressing, top-dressing, inoculation of microorganisms and consortium with soybean/maize were applied. In T2, T3, T4, and T5, we applied the same practices of T1, except subsoiling (T2), basal dressing (T3), top-dressing (T4) and inoculation of microorganisms (T5). T6 was the control, which used none of these practices, including the consortium with soybean/maize. The results indicate that the highest rate of plant survival occurred in T2, while T3 and T4 promoted greater intraspecific competition, compromising the growth in dbh and the yield (m³ ha⁻¹) of plants in future ages. Growth in dbh and th and the yield of plants in the soybean/maize consortium period was higher in T2 and T6. In future ages, the dbh and yield of plants demonstrated higher growth trends in T6, T1, and T5. Agroforestry practices of soil management influence the growth and yield of paricá plants. However, there is a tendency for greater growth and yield for paricá plants cultivated in the absence of agroforestry practices for soil management proposed in this case study. When opting for AFS (paricá intercropped with soybean and maize), it is recommended for paricá a subsoiling, fertilization, and inoculation of microorganisms. Full article

Bovine breeding began in Peru more than five centuries ago; since then, adaptation has started. Nowadays, Creole cattle are part of economic activities of Peruvian farmers, as they supply food and profits, among other things, for families in Andean region. Creole cattle have many strengths such as resistance to diseases, low nutrient requirements and easy adaptation to different levels of altitude and environmental conditions. However, even with all these attributes, they are not valued within production systems. Moreover, these valuable genetic resources are disappearing gradually in Perú. For this reason, this study aims to characterize the zoometry of Creole cows from the Southern Amazonas region of Peru. Biotype classification was performed by hierarchical cluster and multivariate factors analysis. Qualitative traits were analyzed with descriptive statistics and Duncan’s test (α = 0.05) was used to compare means among groups. Associations between qualitative traits were determined with Chi-square. We distinguished three biotypes of Creole cows with characteristics for meat and milk production. This information is helpful for future conservation programs for Creole cattle. Full article

The existence of degraded forests is common in the Eastern Amazon. The maintenance of these forests standing and the recovery of their productivity play an important role in the conservation of biodiversity, storage and carbon sequestration. However, the management techniques currently employed are designed for natural forests in the first harvest cycle or lightly explored and do not apply adequately to forests that have gone through several harvest cycles. Therefore, adaptations and the establishment of new management criteria that take into account other characteristics of these types of forests are necessary to ensure their sustainability. The objective of this study was to propose a silvicultural management system that has the potential to recover and perpetuate the productivity of an intensively logged tropical forest. A forest census was carried out on 535.6 ha for trees with dbh ≥ 25 cm. With these data, the following two treatments were designed: (1) criteria: the BDq method was applied from B = 9.8 m² ha⁻¹, D = 100 cm and q = 2. The criteria for standing wood commercialization were, in this order, first, Health; second, Tree Stem; third, Tree Density and fourth, dbh ≥ 105 cm. (2) Control: the planning was in accordance with Brazilian regulations. For the cost–benefit and sensitivity analysis, the Net Present Value (NPV) was used and a projection of ±20% was made in the commercial price of standing wood. In the criteria treatment, a higher number of trees and species destined for the commercialization of standing wood was verified in relation to the control treatment, showing a greater diversity of species. In the criteria treatment, NPV was positive and superior to the control treatment in all scenarios. The proposed silvicultural management system with an object of an explored and enriched forest, with criteria for harvesting trees with a minimum cut diameter of 25 cm, proved to be viable to generate economic returns and with conservationist potential for the continuous supply of forest products and maintaining biodiversity. Full article

Studies on the vertical distribution of arthropods in temperate forests have revealed the uneven vertical distribution of communities. Many factors influence these patterns simultaneously. However, there are still many questions related to the vertical distribution of Coleoptera in deciduous forests of the temperate zone. The research was carried out within the territory of the Republic of Mordovia (the center of the European part of Russia). Fermental traps with a bait made of fermenting beer with sugar were used to collect Coleoptera. The collections were carried out from May to September 2020 at five sites in a deciduous forest. We set traps at a height of 1.5, 3.5, 7.5 and 12 m above the ground) on the branches of trees. Ninety-two species were identified at the end of studies at different heights. The families Nitidulidae (15 species), Cerambycidae (14 species), Elateridae (7 species), Curculionidae (7 species) and Scarabaeidae (7 species) had the greatest species diversity. The greatest species diversity was recorded at a height of 1.5 m, while the smallest one was recorded at a height of 7.5 m. The minimum number of specimens was recorded at a height of 12 m. The largest differences in the Jaccard similarity index were obtained between samples from a height of 1.5 and 12 m. The Shannon’s diversity index was higher near the ground than in the tree crowns (at heights of 7.5 and 12 m), and the Simpson index had the opposite tendency. Glischrochilus hortensis and to a lesser extent Cychramus luteus preferred to live in the lowest layers of deciduous forest (1.5 m). Cryptarcha strigata was mainly found with relatively high numbers at heights of 3.5 m and 7.5 m. The abundance and occurrence of Protaetia marmorata and Quedius dilatatus were higher in the uppermost layers of the crowns. The number of saproxylic beetle species at heights of 3.5–12 m was almost the same, while in the surface layer it decreased. The number of anthophilic beetle species was also lower at a low altitude. Our data confirm the relevance of sampling in forest ecosystems at different altitudes while studying arthropod biodiversity. Full article

The continental shelf of Brazil is home to a wide range of lobster species, with varying body size, color, habitat preference, and geographic and bathymetric distribution. Spiny lobsters (Panulirus) and slipper lobsters (Scyllaridae) are exploited for export and for the domestic market. Deep sea lobsters (Nephropidae and Polychelidae) have no commercial potential, and little is known about their biology. In this review, we identified 24 lobster species from benthic ecosystems off Brazil (Palinuridae 25%, Scyllaridae 29%, Nephropidae 25%, Polychelidae 17%, Enoplometopidae 4%). We designed a simplified theoretical scheme to understand the role of lobsters in the ecosystem, based on available evidence of distribution, biodiversity, life cycle, connectivity, and abundance. Finally, we propose a theoretical scheme of trophic top-down control, with interactions between a large decapod (spiny lobster), a demersal predator (red snapper), an apex predator (small tuna), benthic invertebrates and fishing exploitation. Full article