Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
3.7
3.7
Journals
Fermentation
Special Issues
Process Intensification in Microbial Biotechnology
share announcement

Process Intensification in Microbial Biotechnology

A special issue of Fermentation (ISSN 2311-5637). This special issue belongs to the section "Fermentation Process Design".

Deadline for manuscript submissions: closed (28 February 2022) | Viewed by 7277

Special Issue Editors

Prof. Dr. Oliver Spadiut

E-Mail Website
Guest Editor
Research Division Biochemical Engineering, Institute of Chemical, Environmental and Bioscience Engineering, TU Wien, Vienna, Austria
Interests: escherichia coli; protein; bioengineering
Dr. Julian Quehenberger

E-Mail Website
Guest Editor
Research Division Biochemical Engineering, Institute of Chemical, Environmental and Bioscience Engineering, Faculty of Technical Chemistry, TU Wien, 1060 Vienna, Austria
Interests: biochemical engineering; bioprocess development
Dr. David Wurm

E-Mail Website
Guest Editor
Research Division Biochemical Engineering, Institute of Chemical, Environmental and Bioscience Engineering, TU Wien, Vienna, Austria
Interests: escherichia coli; recombinant protein production; bioprocess development；inclusion bodies
Dr. Julian Kopp

E-Mail Website
Guest Editor
Institute of Chemical Engineering, TU Wien, 1040 Vienna, Austria
Interests: microbial hosts; fermentation; continuous biomanufacturing; single-cell analytics; process intensification; inclusion bodies; population heterogeneities
Dr. Stefan Pflügl

E-Mail Website
Guest Editor
Institute for Chemical, Environmental and Bioscience Engineering, Research Area Biochemical Engineering, Technische Universität Wien, Gumpendorfer Straße 1a, 1060 Vienna, Austria
Interests: microbial bioprocess and strain development; continuous bioprocessing; extremophiles; gas fermentation; metabolic engineering
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Process Intensification (PI) is a major trend in modern industrial biotechnology. The concept of PI was originally developed in the chemical industry and is described as "any chemical engineering development that leads to a substantially smaller, cleaner, safer and more energy efficient technology", according to the definition by Stankiewicz and Moulijn (2000). PI aims to establish sustainable and cost-effective solutions that can be realized via ground-breaking changes through an integration of technologies, operation simplification and/or insights via analytics and model-based approaches. PI plays a key role in ensuring competitiveness in an increasingly contested global biomanufacturing market, but might also be the needed break-through that allows biorefinery concepts to become a serious competition for the traditional petrochemical industry. The Special Issue "Process Intensification in Microbial Biotechnology in Fermentation seeks contributions that assess the state-of-the-art and future developments in the field of PI. Submissions may include, but are not limited to novel concepts for combination of unit operations, new reactor and cultivation technologies, co-culture fermentation systems, in situ product removal/recovery, continuous upstream and downstream processing, as well as analytics and model-based approaches that are connected to the aforementioned areas.

Prof. Dr. Oliver Spadiut
Dr. Julian Quehenberger
Dr. David Wurm
Dr. Julian Kopp
Dr. Stefan Pflügl
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Fermentation is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

 

Keywords

  • process intensification
  • process integration
  • in situ product removal
  • combination of unit operations
  • co-culture fermentations
  • integrated bioprocess development
  • continuous cultivation
  • continuous downstream processing
  • microbial hosts

Published Papers (2 papers)

Download All Papers
Order results
Result details
Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:

Research

Jump to: Review

18 pages, 2200 KiB  
Article
Optimized Operating Conditions for a Biological Treatment Process of Industrial Residual Process Brine Using a Halophilic Mixed Culture
by Thomas Mainka, Christoph Herwig and Stefan Pflügl
Fermentation 2022, 8(6), 246; https://0-doi-org.brum.beds.ac.uk/10.3390/fermentation8060246 - 25 May 2022
Cited by 1 | Viewed by 2462
Abstract
Residual process brine is a sustainable raw material for chlor-alkali electrolysis processes. This study investigates the influence of critical process parameters on the performance of a continuous treatment process for residual process brine using halophilic microorganisms. The goal of the bioprocess is an [...] Read more.
Residual process brine is a sustainable raw material for chlor-alkali electrolysis processes. This study investigates the influence of critical process parameters on the performance of a continuous treatment process for residual process brine using halophilic microorganisms. The goal of the bioprocess is an efficient degradation of the organic impurities formate, aniline, phenol, and 4,4′-methylenedianline from this residual stream. It was shown that formate could be degraded with high efficiencies (89–98%) during the treatment process. It was observed that formate degradation was influenced by the co-substrate glycerol. The lowest residual formate concentrations were achieved with specific glycerol uptake rates of 8.0–16.0 × 10−3 g L−1 h−1 OD600−1. Moreover, a triple-nutrient limitation for glycerol, ammonium, and phosphate was successfully applied for continuous cultivations. Furthermore, it was shown that all aromatic impurities were degraded with an efficiency of 100%. Ultimately, this study proposed optimized operating conditions, allowing the efficient degradation of organics in the residual process brine under various process conditions. Future optimization steps will require a strategy to prevent the accumulation of potential intermediate degradation products formed at high aniline feed concentrations and increase the liquid dilution rates of the system to achieve a higher throughput of brines. Full article
(This article belongs to the Special Issue Process Intensification in Microbial Biotechnology)
►▼ Show Figures

Figure 1

Review

Jump to: Research

13 pages, 1339 KiB  
Review
Challenges in Expression and Purification of Functional Fab Fragments in E. coli: Current Strategies and Perspectives
by Rucha S. Patil, Anupa Anupa, Jaya A. Gupta and Anurag S. Rathore
Fermentation 2022, 8(4), 175; https://0-doi-org.brum.beds.ac.uk/10.3390/fermentation8040175 - 09 Apr 2022
Cited by 11 | Viewed by 4011
Abstract
Microbial host systems remain the most efficient and cost-effective chassis for biotherapeutics production. Escherichia coli is often the preferred host due to ease of cloning, scale-up, high product yields, and most importantly, cost-effective cultivation. E. coli often experience difficulties in producing biologically active [...] Read more.
Microbial host systems remain the most efficient and cost-effective chassis for biotherapeutics production. Escherichia coli is often the preferred host due to ease of cloning, scale-up, high product yields, and most importantly, cost-effective cultivation. E. coli often experience difficulties in producing biologically active therapeutics such as Fab fragments, which require protein folding and subsequent three-dimensional structure development. This paper outlines the recent improvements in upstream and downstream unit operations for producing Fab fragments in E. coli. Monoclonal antibody fragments (Fab) are a rising class of biotherapeutics and their production has been optimised using coexpression of molecular chaperones such as DsbC or DnaK–DnaJ–GrpE, as well as strain engineering for post-translational modifications such as disulphide bridging. Different media systems such as EnBase and combining nitrogen source supplementation with low-temperature cultivation have resulted in improvement in cell integrity, protein expression, and protein refolding. The recovery of native proteins from insoluble inclusion bodies can be improved by adjusting refolding conditions, as well as by incorporating multimodal and affinity chromatography for achieving high product yields in purification. Recent developments summarised in this review may tune the E. coli expression system to produce more complex and glycosylated proteins for therapeutic use in the near future. Full article
(This article belongs to the Special Issue Process Intensification in Microbial Biotechnology)
►▼ Show Figures

Figure 1

Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:
 
Displaying articles 1-2
Back to TopTop