Meat Safety Research: Microbial Risk Analysis and Foodborne Pathogens Control

A special issue of Foods (ISSN 2304-8158). This special issue belongs to the section "Meat".

Deadline for manuscript submissions: closed (30 June 2022) | Viewed by 19548

Special Issue Editors


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Guest Editor
Department of Food Safety, Nutrition and Veterinary Public Health, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy
Interests: food microbiology; food-borne bacterial pathogens; rapid detection of foodborne pathogens; molecular methods in food microbiology
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
Department Food Safety, Nutrition and Veterinary Public Health, Istituto Superiore di Sanità (ISS) , Viale Regina Elena 299, I-00161 Rome, Italy
Interests: food microbiology; food-borne bacterial pathogens; rapid detection of foodborne pathogens; molecular methods in food microbiology; food-borne viruses
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Meat products are recognized as one of the most perishable foods favoring microbial growth. A variety of bacteria, as well as viruses and parasites, can contaminate meat products, and a high percentage of food is wasted due to microbial alteration. Meat represents the main vehicle of foodborne pathogens, and it is implicated in certain foodborne outbreaks. A significant number of pathogens can be transmitted to humans through meat products, but the risks can be mitigated. The prevalence of some traditional pathogens has been reduced, while others have persisted for decades, and new ones, often more-virulent or antibiotic-resistant strains of existing microorganisms, are emerging over time. Microbiological food controls play a fundamental role in ensuring the food safety of meat products.

This Special Issue encourages the submission of original research or reviews of the scientific literature addressing microbial pathogens and their control through the implementation of food safety programs in the meat production chain.

This Special Issue will address the recent advances in molecular-based technologies, including advances in microbial quantitative detection methodologies, developed with the aim of further elucidating how microbial foodborne hazards contaminate, survive and persist in meat and in the food chain, or helping producers and food safety authorities in the microbiological control of the safety of food lots.

The Special Issue of Foods will also address studies on the microbiological molecular methods used in epidemiological studies for outbreak investigation and source attribution. The application of molecular-based subtyping methods, including pulsed field gel electrophoresis (PFGE), multi-locus sequence typing (MLST), multi-locus variable number of tandem repeats analysis (MLVA) and, more recently, whole genome sequencing (WGS), has recently replaced phenotype-based testing for the subtyping of microbial pathogens, providing a greater level of discrimination and improving the identification of transmission pathways.

Studies concerning the phylogenesis of the main important foodborne pathogens will also be welcomed. Clear and concisely written manuscripts will be highly desirable and appreciated.

Dr. Elisabetta Delibato
Dr. Dario De Medici 
Guest Editors

Manuscript Submission Information

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Foods is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2900 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • meat safety
  • foodborne pathogens
  • molecular technologies

Published Papers (3 papers)

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Research

9 pages, 1197 KiB  
Communication
Application of a Loop-Mediated Isothermal Amplification (LAMP) Assay for the Detection of Listeria monocytogenes in Cooked Ham
by Alfonsina Fiore, Ida Treglia, Gianni Ciccaglioni, Marco Francesco Ortoffi and Antonietta Gattuso
Foods 2023, 12(1), 193; https://0-doi-org.brum.beds.ac.uk/10.3390/foods12010193 - 01 Jan 2023
Cited by 2 | Viewed by 2381
Abstract
Changing eating habits and rising demand of food have increased the incidence of foodborne diseases, particularly in industrialized countries. In this context, contaminated ready-to-eat food (RTE) may be a vehicle for the transmission of Listeria monocytogenes (L. monocytogenes), a foodborne pathogen [...] Read more.
Changing eating habits and rising demand of food have increased the incidence of foodborne diseases, particularly in industrialized countries. In this context, contaminated ready-to-eat food (RTE) may be a vehicle for the transmission of Listeria monocytogenes (L. monocytogenes), a foodborne pathogen responsible of listeriosis, a severe infectious disease involving humans and animals. It would be useful to have rapid detection methods to screen the presence of L. monocytogenes in food. In this study, a colorimetric Loop-mediated isothermal amplification (LAMP) assay was applied to the detection of L. monocytogenes in 37 experimentally contaminated RTE meat samples. The LAMP primers consisted of a set of six primers targeting eight regions on the hlyA gene; the assay was carried out in 30 min at 65 °C in a water bath. Amplification products were visualized by color change assessment. The results of colorimetric LAMP assays based on the hly gene obtained in this study were compared to microbiological cultural methods, real-time PCR and real-time LAMP PCR, which show 100% specificity and sensitivity. These data suggest that colorimetric LAMP assays can be used as a screen to detect L. monocytogenes in ready-to-eat meat food. Full article
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14 pages, 3097 KiB  
Article
Prevalence, Virulence Genes, Phylogenetic Analysis, and Antimicrobial Resistance Profile of Helicobacter Species in Chicken Meat and Their Associated Environment at Retail Shops in Egypt
by Amina Mohamed Elrais, Walid S. Arab, Khalid Ibrahim Sallam, Walaa Abd Elmegid, Fatma Elgendy, Walid Elmonir, Kálmán Imre, Adriana Morar, Viorel Herman and Haitham Elaadli
Foods 2022, 11(13), 1890; https://0-doi-org.brum.beds.ac.uk/10.3390/foods11131890 - 26 Jun 2022
Cited by 6 | Viewed by 2108
Abstract
Helicobacter pylori (H. pylori) and Helicobacter pullorum (H. pullorum) are frequently reported pathogens in humans and poultry, respectively. Nevertheless, the source of H. pylori is still unclear. This study aimed to detect Helicobacter spp. in chicken carcasses and to [...] Read more.
Helicobacter pylori (H. pylori) and Helicobacter pullorum (H. pullorum) are frequently reported pathogens in humans and poultry, respectively. Nevertheless, the source of H. pylori is still unclear. This study aimed to detect Helicobacter spp. in chicken carcasses and to assess the antibiogram and the virulence genes of Helicobacter isolates. Three hundred chicken meat samples (100 each of chicken breast, liver, and gizzard), besides 60 swab samples from chicken processing surfaces, were collected from retail shops in Qalyubia Governorate, Egypt, and examined for the prevalence of H. pylori and H. pullorum. The 16S rRNA of three H. pylori and two H. pullorum isolates were sequenced to determine the genetic relationship between these two Helicobacter spp. Of the 300 chicken samples tested, 16 (5.33%) and 14 (4.67%) were positive for H. pylori and H. pullorum, respectively. Multiplex PCR revealed that the virulence genes vacuolating cytotoxin A (vacA)s1, cytotoxin-associated gene A (cagA), and restriction endonuclease-replacing gene A (hrgA) were detected in 66.7%, 77.8%, and 100% of H. pylori strains tested, respectively. H. pylori showed the highest resistance for clarithromycin, while H. pullorum exhibited the highest resistance towards erythromycin and ciprofloxacin. The study concluded that the chicken meat and giblets are potential sources of the virulent and antimicrobial-resistant strains of H. pylori of human origin. Full article
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15 pages, 1659 KiB  
Article
SARS-CoV-2 Remains Infectious on Refrigerated Deli Food, Meats, and Fresh Produce for up to 21 Days
by Mo Jia, Tina M. Taylor, Sterling M. Senger, Reza Ovissipour and Andrea S. Bertke
Foods 2022, 11(3), 286; https://0-doi-org.brum.beds.ac.uk/10.3390/foods11030286 - 21 Jan 2022
Cited by 25 | Viewed by 14152
Abstract
SARS-CoV-2, the virus that causes COVID-19, has been detected on foods and food packaging and the virus can infect oral cavity and intestinal cells, suggesting that infection could potentially occur following ingestion of virus-contaminated foods. To determine the relative risk of infection from [...] Read more.
SARS-CoV-2, the virus that causes COVID-19, has been detected on foods and food packaging and the virus can infect oral cavity and intestinal cells, suggesting that infection could potentially occur following ingestion of virus-contaminated foods. To determine the relative risk of infection from different types of foods, we assessed survival of SARS-CoV-2 on refrigerated ready-to-eat deli items, fresh produce, and meats (including seafood). Deli items and meats with high protein, fat, and moisture maintained infectivity of SARS-CoV-2 for up to 21 days. However, processed meat, such as salami, and some fresh produce exhibited antiviral effects. SARS-CoV-2 also remained infectious in ground beef cooked rare or medium, but not well-done. Although infectious SARS-CoV-2 was inactivated on the foods over time, viral RNA was not degraded in similar trends, regardless of food type; thus, PCR-based assays for detection of pathogens on foods only indicate the presence of viral RNA, but do not correlate with presence or quantity of infectious virus. The survival and high recovery of SARS-CoV-2 on certain foods support the possibility that food contaminated with SARS-CoV-2 could potentially be a source of infection, highlighting the importance of proper food handling and cooking to inactivate any contaminating virus prior to consumption. Full article
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