Gene Editing in Animal System

A special issue of Genes (ISSN 2073-4425). This special issue belongs to the section "Animal Genetics and Genomics".

Deadline for manuscript submissions: closed (20 March 2022) | Viewed by 2382

Special Issue Editor

College of Animal Science, South China Agricultural University, Guangzhou, China
Interests: gene editing; transgenesis; animal genetics; animal breeding; animal cloning; pigs

Special Issue Information

Dear Colleagues,

The currently emerging gene editing techniques have shown promise for developing genetically modified model and farm animals for biomedical and agricultural studies. The Special Issue focuses on advances in genome editing techniques and their applications in animal systems. The gene editing methods and tools mentioned above can include traditional homologous recombination; popular engineered nucleases, such as ZFN, TALEN, CRISPR, and base editors; and newly emerging tools. The studies we have collected include the development and optimization of gene editing approaches applicable to animal cells, embryos, and tissues; the application of gene editing techniques for genetic modifications of animals for biomedicine and agricultural purposes; and gene therapy in animal models via the delivery of engineered nucleases for in vivo genome editing. We also welcome articles discussing current regulations of gene-edited livestock for human use and consumption.

Prof. Dr. Huaqiang Yang
Guest Editor

Manuscript Submission Information

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Keywords

  • gene editing
  • animal models
  • livestock
  • animal breeding
  • CRISPR
  • genome engineering
  • genetic modification
  • gene therapy
  • stem-cell therapy
  • genetic diseases

Published Papers (1 paper)

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Research

11 pages, 1730 KiB  
Article
Simple and Rapid Assembly of TALE Modules Based on the Degeneracy of the Codons and Trimer Repeats
by Lingyin Cheng, Xiaoqing Zhou, Yuling Zheng, Chengcheng Tang, Yu Liu, Shuwen Zheng, Yang Liu, Jizeng Zhou, Chuan Li, Min Chen, Liangxue Lai and Qingjian Zou
Genes 2021, 12(11), 1761; https://0-doi-org.brum.beds.ac.uk/10.3390/genes12111761 - 05 Nov 2021
Cited by 4 | Viewed by 1987
Abstract
Transcription activator-like effectors (TALEs) have been effectively used for targeted genome editing, transcriptional regulation, epigenetic modification, and locus-specific DNA imaging. However, with the advent of the clustered regularly interspaced short palindromic repeat/Cas9 system, an easy-to-use tool with the same function as TALEs, TALEs [...] Read more.
Transcription activator-like effectors (TALEs) have been effectively used for targeted genome editing, transcriptional regulation, epigenetic modification, and locus-specific DNA imaging. However, with the advent of the clustered regularly interspaced short palindromic repeat/Cas9 system, an easy-to-use tool with the same function as TALEs, TALEs have recently been abandoned because of their complexity, time consumption, and difficult handling in common labs. Here, we described a degenerated codon-based TALE assembly system for simple, rapid, and efficient TALE assembly. TALE trimers with nonrepetitive DNA sequences were amplified by PCR and sequentially assembled via Gibson assembly. Our method is cost-effective, requires only commonly used basic molecular biology reagents, and takes only 2 h from target sequence analysis to completion. This simple, rapid, and lab-friendly TALE assembly method will restore the value of TALEs in DNA targeting. Full article
(This article belongs to the Special Issue Gene Editing in Animal System)
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