Plant Microbiome: Structure and Function

A special issue of Genes (ISSN 2073-4425). This special issue belongs to the section "Microbial Genetics and Genomics".

Deadline for manuscript submissions: closed (20 September 2021) | Viewed by 3236

Special Issue Editors


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Guest Editor
Estación Experimental del Zaidín, Spanish National Research Council (CSIC), 1, 18008 Granada, Spain
Interests: bioremediation; biodegradation; emerging contaminants; explosives; PAHs; pesticides; microbiology; microbial biodiversity
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Guest Editor
Centre for Environmental Sciences, Hasselt University, Martelarenlaan 42, 3500 Hasselt, Belgium
Interests: plant–microbiome interactions; abiotic stress; pollution; phytoremediation; bioremediation; plant growth promotion

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Guest Editor
Centre for Environmental Sciences, Hasselt University, Martelarenlaan 42, 3500 Hasselt, Belgium
Interests: endophytes; plant growth promoting (rhizo)bacteria; pollution; phytoremediation; metagenomics; plant–microbe interactions; bioaugmentation; biostimulation

Special Issue Information

Dear Colleagues,

Plant-associated microbiota are important determinants for plant health in the environment, with important implications for global geochemical cycles, ecosystem functioning and sustainable agriculture. Plant microbiomes include those microbial communities associated with the rhizosphere, the phyllosphere (leaves and aerial parts), and those found within plant tissues (the endosphere). Members of these complex communities, which can be bacteria, archaea and fungi, may assist plants in obtaining nutrients, promote its growth, and help plants to resist pathogens, inorganic and organic pollutants and adverse environmental conditions. On the contrary, these communities may also house harmful plant pathogens.

Thanks to increased accessibility to new sequencing techniques, the study of plant microbiomes by sequencing amplicon libraries of taxonomic marker genes, principally the 16S rRNA gene, has led to an enormous boost in our knowledge of the structure of plant microbiomes. However, functional studies from which it can be determined what the microbial communities ‘do’ when associated with plants remain less well studied. This is especially important with regard to increasing our understanding of how members of the microbiome aid plant growth and health when plants are faced with adverse biotic or abiotic conditions.

In this Special Issue, we seek original research and reviews which deepen our understanding of both the microbial structure and function of microbiomes of plants in natural environments or agriculture under different conditions.

Dr. Pieter van Dillewijn
Prof. Jaco Vangronsveld
Dr. Sofie Thijs
Guest Editors

Manuscript Submission Information

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Keywords

  • Rhizosphere
  • Phyllosphere
  • Endosphere
  • Plants
  • Plant–microbe interactions
  • Microbiome
  • Microbial communities
  • Sequencing technology
  • rRNA

Published Papers (1 paper)

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Research

14 pages, 2043 KiB  
Article
Impact of Harvest on Switchgrass Leaf Microbial Communities
by Esther Singer, Elizabeth M. Carpenter, Jason Bonnette, Tanja Woyke and Thomas E. Juenger
Genes 2022, 13(1), 22; https://0-doi-org.brum.beds.ac.uk/10.3390/genes13010022 - 22 Dec 2021
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Abstract
Switchgrass is a promising feedstock for biofuel production, with potential for leveraging its native microbial community to increase productivity and resilience to environmental stress. Here, we characterized the bacterial, archaeal and fungal diversity of the leaf microbial community associated with four switchgrass ( [...] Read more.
Switchgrass is a promising feedstock for biofuel production, with potential for leveraging its native microbial community to increase productivity and resilience to environmental stress. Here, we characterized the bacterial, archaeal and fungal diversity of the leaf microbial community associated with four switchgrass (Panicum virgatum) genotypes, subjected to two harvest treatments (annual harvest and unharvested control), and two fertilization levels (fertilized and unfertilized control), based on 16S rRNA gene and internal transcribed spacer (ITS) region amplicon sequencing. Leaf surface and leaf endosphere bacterial communities were significantly different with Alphaproteobacteria enriched in the leaf surface and Gammaproteobacteria and Bacilli enriched in the leaf endosphere. Harvest treatment significantly shifted presence/absence and abundances of bacterial and fungal leaf surface community members: Gammaproteobacteria were significantly enriched in harvested and Alphaproteobacteria were significantly enriched in unharvested leaf surface communities. These shifts were most prominent in the upland genotype DAC where the leaf surface showed the highest enrichment of Gammaproteobacteria, including taxa with 100% identity to those previously shown to have phytopathogenic function. Fertilization did not have any significant impact on bacterial or fungal communities. We also identified bacterial and fungal taxa present in both the leaf surface and leaf endosphere across all genotypes and treatments. These core taxa were dominated by Methylobacterium, Enterobacteriaceae, and Curtobacterium, in addition to Aureobasidium, Cladosporium, Alternaria and Dothideales. Local core leaf bacterial and fungal taxa represent promising targets for plant microbe engineering and manipulation across various genotypes and harvest treatments. Our study showcases, for the first time, the significant impact that harvest treatment can have on bacterial and fungal taxa inhabiting switchgrass leaves and the need to include this factor in future plant microbial community studies. Full article
(This article belongs to the Special Issue Plant Microbiome: Structure and Function)
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