RNA Silencing in Plant–Microorganism Interactions

A special issue of Genes (ISSN 2073-4425). This special issue belongs to the section "Microbial Genetics and Genomics".

Deadline for manuscript submissions: closed (25 January 2022) | Viewed by 3245

Special Issue Editors


E-Mail Website
Guest Editor
Department of Biotechnology, University of Verona, 37129 Verona, Italy
Interests: plant-symbiont interactions; functional genomics; gene silencing; transgenesis

E-Mail Website
Guest Editor
Department of Biotechnology, University of Verona, 37129 Verona, Italy
Interests: plant biotechnology; solanum lycopersicum; parthenocarpy; fruit growth and development; phytohormones

Special Issue Information

Dear Colleagues,

RNA silencing is a regulatory process that in eukaryotic cells controls the expression of target mRNAs and their translation. The effector molecules of RNA silencing are small non-coding RNAs (sRNAs) resulting from the processing of dsRNA molecules derived from miRNA-encoded genes or transcripts of different origins containing inverted repeats. Effector molecules ensure mechanism specificity by guiding the RNA-induced silencing complex (RISC) to the target mRNA in a sequence-specific manner. In plants, RNA silencing is involved in defense against viruses through a process that, triggered by viral-derived dsRNAs, leads to inhibition of virus replication. Recently, it has been shown that plant immunity and fungal virulence may involve a communication mechanism based on sRNAs traveling between plants and fungal cells, called cross-kingdom RNA interference (RNAi). Evidence for cross-kingdom communication based on the exchange of silencing-inducing RNA molecules has also been more recently demonstrated for symbiotic interaction in plants. In this special issue, we invite the presentation of original articles, short communications, and reviews covering different aspects of the RNA silencing mechanism in controlling plant-microorganism interactions, including applications of RNAi technology in plant protection and symbiotic association

Prof. Dr. Tiziana Pandolfini
Dr. Barbara Molesini
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Genes is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • RNA silencing
  • miRNA
  • Plant defense to pathogens
  • Plant-symbiont interaction
  • Host-induced gene silencing
  • Spray-induced gene silencing
  • Small non coding RNAs
  • Cross-kingdom communication
  • RNAi technology

Published Papers (1 paper)

Order results
Result details
Select all
Export citation of selected articles as:

Research

18 pages, 3839 KiB  
Article
Successful Silencing of the Mycotoxin Synthesis Gene TRI5 in Fusarium culmorum and Observation of Reduced Virulence in VIGS and SIGS Experiments
by Polina Tretiakova, Ralf Thomas Voegele, Alexander Soloviev and Tobias Immanuel Link
Genes 2022, 13(3), 395; https://0-doi-org.brum.beds.ac.uk/10.3390/genes13030395 - 23 Feb 2022
Cited by 6 | Viewed by 2674
Abstract
Crops constantly experience various biotic stresses during their life cycle, and Fusarium spp. remain one of the most serious groups of pathogens affecting plants. The ability to manipulate the expression of certain microorganism genes via RNAi creates the opportunity for new-generation dsRNA-based preparations [...] Read more.
Crops constantly experience various biotic stresses during their life cycle, and Fusarium spp. remain one of the most serious groups of pathogens affecting plants. The ability to manipulate the expression of certain microorganism genes via RNAi creates the opportunity for new-generation dsRNA-based preparations to control a large number of diseases. In this study, we applied virus-induced gene silencing (VIGS), and spray-induced gene silencing (SIGS) to silence the trichothecene-producing gene TRI5 in F. culmorum as a means to reduce its aggressiveness on spring wheat. Treatment of the fungus with dsTRI5RNA in vitro reduced deoxynivalenol (DON) and 3-acetyldeoxynivalenol (3-A-DON) accumulations by 53–85% and 61–87%, respectively, and reduced TRI5 expression by 84–97%. VIGS decreased the proportion of infected wheat spikelets by 73%, but upregulation was observed for TRI5. SIGS on wheat leaves and ears using certain dsTRI5RNA amounts negatively impacted F. culmorum growth. However, when performing in vivo analyses of TRI5 mRNA levels, the upregulation of the gene was determined in the variants where fungal colonization was restricted, suggesting a compensatory reaction of the pathogen to RNAi. Full article
(This article belongs to the Special Issue RNA Silencing in Plant–Microorganism Interactions)
Show Figures

Figure 1

Back to TopTop