Special Issue "Next Generation DNA Sequencing"
Deadline for manuscript submissions: closed (31 May 2010).
Interests: microbial genomics; technology development; infectious diseases; gene expression
We are currently in the midst of a revolution in DNA sequencing that promises unprecedented access to genetic data. Next Generation Sequencing (NGS) and follow-on technologies such as single molecule sequencing (next-next gen or 3rd generation) instruments and methods are advancing rapidly. These advances open the door to new applications for sequencing such as large-scale association studies, comparative genomics, metagenomics and the study of ancient DNA. These technologies have also been used in chromatin immunoprecipitation studies to determine DNA binding sites and quantitative RNA expression analysis by sequencing. These instrument platforms create unique challenges both upstream and downstream of the data generation. Improved methods for library construction and amplification strategies to produce more uniform and complete coverage are required. Methods are also evolving for the processing and analysis of extremely large numbers (often short) reads to better understand quality, improve alignments and generate whole-genome assemblies. Given the huge impact of these developments, a special issue of the journal Genes is being issued to explore the methods and applications of these new sequencing technologies. Authors are encouraged to submit original manuscripts describing utilization of Next Generation Sequencing to answer scientific questions. Also encouraged are papers describing new methods or instruments, and reviews or comparisons of Next Generation Sequencing technologies as well as manuscripts dealing with bioinformatic methods of analysis.
Dr. Paul Richardson
- DNA Sequencing
- next generation sequencing
- single molecule sequencing
- massively parallel sequencing
- ancient DNA
- digital gene expression