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State-of-the-Art Biochemistry
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State-of-the-Art Biochemistry

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Biochemistry".

Deadline for manuscript submissions: closed (31 July 2022) | Viewed by 5276

Special Issue Editors

Dr. Amedeo Amedei

E-Mail Website
Collection Editor
Department of Experimental and Clinical Medicine, University of Florence, 50134 Florence, Italy
Interests: microbiota-immunity axis; autoimmunity; cancers; inflammation; T cells; micro and nanoplastic effects on human
Special Issues, Collections and Topics in MDPI journals
Dr. Mauro Salvi

E-Mail Website
Collection Editor
Department of Biomedical Sciences, University of Padova, Padova, Italy
Interests: protein phosphorylation; acidic protein kinases; tyrosine kinases; kinase inhibitors, signal transduction; post-translational modifications; cancer; cystic fibrosis
Special Issues, Collections and Topics in MDPI journals
Dr. Claudia Fiorillo

E-Mail Website
Collection Editor
Department of Biomedical, Experimental and Clinical Sciences “Mario Serio”, University of Florence, Viale Morgagni 50, 50134 Florence, Italy
Interests: redox markers; antioxidant capacity; post-translational oxidative modification; ROS sources
Special Issues, Collections and Topics in MDPI journals
Prof. Dr. Massimo Donadelli

E-Mail Website
Collection Editor
Department of Neurosciences, Biomedicine and Movement Sciences, Section of Biochemistry Strada Le Grazie 8, University of Verona, 7134 Verona, Italy
Interests: cancer; ROS; metabolism; p53; chemoresistance
Prof. Dr. Giorgina Piccoli

E-Mail Website
Collection Editor
1. Nephrology, Centre Hospitalier Le Mans, Le Mans, France
2. Department of Clinical and Biological Sciences, ASOU San Luigi, University of Torino, 10043 Orbassano, Italy
Interests: nephrology; pregnancy in the context of chronic diseases; nutrtional issues in nephrology and pregnacy
Dr. Daniela Impellizzeri

E-Mail Website
Collection Editor
Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Via F. Stagno D’Alcontres 31, 98166 Messina, Italy
Interests: inflammation; oxidative stress; biochemistry; pharmacology; molecular biology
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

This Regional Project aims to collect high-quality research articles, review articles, and communications on all aspects of biochemistry from Italy. We encourage the submission of manuscripts that provide novel and mechanistic insights and papers that report significant advances in the fields.

The areas of interest for the regional collection include but are not limited to topics such as:

  • Cellular function and structure
  • Cell signaling
  • Cell Metabolism
  • Proteostasis and Disease
  • Protein biosynthesis
  • Gene and protein structure and expression
  • Cancer pathology and biology
  • Aging biology and age-related diseases
  • Drugs and pharmaceutics
  • Membrane function
  • Post-translational modifications in Health and Disease
  • New approaches in the management of hypoxic tumors
  • Cancer molecular genetics
  • Enzymology and structural biology
  • Metalloenzymes
  • Enzyme activation and inhibition
  • Targeting human enzymes involved in tumorigenesis
  • Function and structure of protein membrane and drug distribution in the body
  • Protein interactions and functional nucleic acids
  • Epigenetic and genetic regulatory mechanisms
  • Lipid metabolism;
  • Drug resistance
  • Role of intestinal microbes in diseases and human health
  • Characterization and development of small molecules for targeting metabolic pathways essential for the life cycle of human pathogens
  • Cancer metabolism 

Dr. Amedeo Amedei
Dr. Mauro Salvi
Dr. Claudia Fiorillo
Prof. Dr. Massimo Donadelli
Prof. Dr. Giorgina Piccoli
Dr. Daniela Impellizzeri
Collection Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

 

Published Papers (2 papers)

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Research

13 pages, 2758 KiB  
Article
Role of Etanercept and Infliximab on Nociceptive Changes Induced by the Experimental Model of Fibromyalgia
by Marika Cordaro, Rosalba Siracusa, Ramona D’Amico, Tiziana Genovese, Gianluca Franco, Ylenia Marino, Davide Di Paola, Salvatore Cuzzocrea, Daniela Impellizzeri, Rosanna Di Paola and Roberta Fusco
Int. J. Mol. Sci. 2022, 23(11), 6139; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms23116139 - 30 May 2022
Cited by 17 | Viewed by 2045
Abstract
Background: Fibromyalgia is a clinical condition that affects 1% to 5% of the population. No proper therapy has been currently found. It has been described that inflammation plays a central role in the nerve sensitizations that characterize the pathology. Methods: This paper aimed [...] Read more.
Background: Fibromyalgia is a clinical condition that affects 1% to 5% of the population. No proper therapy has been currently found. It has been described that inflammation plays a central role in the nerve sensitizations that characterize the pathology. Methods: This paper aimed to evaluate the efficacy of etanercept and infliximab in the management of pain sensitization. Fibromyalgia was induced by three injections once a day of reserpine at the dose of 1 mg/kg. Etanercept (3 mg/kg) and infliximab (10 mg/kg) were administered the day after the last reserpine injection and then 5 days after that. Behavioral analyses were conducted once a week, and molecular investigations were performed at the end of the experiment. Results: Our data confirmed the major effect of infliximab administration as compared to etanercept: infliximab administration strongly reduced pain sensitization in thermal hyperalgesia and mechanical allodynia. From the molecular point of view, infliximab reduced the activation of microglia and astrocytes and the expression of the purinergic P2X7 receptor ubiquitously expressed on glia and neurons. Downstream of the P2X7 receptor, infliximab also reduced p38-MAPK overexpression induced by the reserpine administration. Conclusion: Etanercept and infliximab treatment caused a significant reduction in pain. In particular, rats that received infliximab showed less pain sensitization. Moreover, infliximab reduced the activation of microglia and astrocytes, reducing the expression of the purinergic receptor P2X7 and p38-MAPK pathway. Full article
(This article belongs to the Special Issue State-of-the-Art Biochemistry)
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15 pages, 1695 KiB  
Article
Systematic Comparison of Beetle Luciferase-Luciferin Pairs as Sources of Near-Infrared Light for In Vitro and In Vivo Applications
by Bruce R. Branchini, Danielle M. Fontaine, Dawn Kohrt, Brian P. Huta, Allison R. Racela, Benjamin R. Fort, Tara L. Southworth and Aldo Roda
Int. J. Mol. Sci. 2022, 23(5), 2451; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms23052451 - 23 Feb 2022
Cited by 6 | Viewed by 2463
Abstract
Luciferases catalyze light-emitting reactions that produce a rainbow of colors from their substrates (luciferins), molecular oxygen, and often additional cofactors. These bioluminescence (BL) systems have afforded an incredible variety of basic research and medical applications. Driven by the importance of BL-based non-invasive animal [...] Read more.
Luciferases catalyze light-emitting reactions that produce a rainbow of colors from their substrates (luciferins), molecular oxygen, and often additional cofactors. These bioluminescence (BL) systems have afforded an incredible variety of basic research and medical applications. Driven by the importance of BL-based non-invasive animal imaging (BLI) applications, especially in support of cancer research, new BL systems have been developed by engineering beetle luciferase (Luc) variants and synthetic substrate combinations to produce red to near-infrared (nIR) light to improve imaging sensitivity and resolution. To stimulate the application of BLI research and advance the development of improved reagents for BLI, we undertook a systematic comparison of the spectroscopic and BL properties of seven beetle Lucs with LH2 and nine substrates, which included two new quinoline ring-containing analogs. The results of these experiments with purified Luc enzymes in vitro and in live HEK293T cells transfected with luc genes have enabled us to identify Luc/analog combinations with improved properties compared to those previously reported and to provide live cell BL data that may be relevant to in vivo imaging applications. Additionally, we found strong candidate enzyme/substrate pairs for in vitro biomarker applications requiring nIR sources with minimal visible light components. Notably, one of our new substrates paired with a previously developed Luc variant was demonstrated to be an excellent in vitro source of nIR and a potentially useful BL system for improved resolution in BLI. Full article
(This article belongs to the Special Issue State-of-the-Art Biochemistry)
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