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Biomedical Optics in Cell Biology
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Biomedical Optics in Cell Biology

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Biophysics".

Deadline for manuscript submissions: closed (31 May 2023) | Viewed by 5765

Special Issue Editors

Prof. Dr. Christoph Cremer

E-Mail Website
Guest Editor
1. Max-Planck Institute for Polymer Research, Mainz, Germany
2. Kirchhoff Institute for Physics, University Heidelberg, Heidelberg, Germany
Interests: advanced light microscopy methods to elucidate biological nanostructures; biophysics of the cell nucleus as the seat of genetic information and of gene regulation; development & biomedical applications of super-resolution methods to overcome the limits of conventional light microscopy, down to the nanometer range of optical and structural resolution
Special Issues, Collections and Topics in MDPI journals
Prof. Dr. Herbert Schneckenburger

E-Mail Website
Guest Editor
Institute of Applied Research, Aalen University, Beethovenstr. 1, 73430 Aalen, Germany
Interests: biomedical optics; laser; microscopy
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Biomedical optics play an increasing role in the diagnosis and treatment of patients, as well as in advanced studies in cell biology. In the latter case, novel methods of 3D microscopy based on various kinds of illumination patterns (wide field, focused laser beam, structured, or light sheet) are adapted for investigations of 3-dimensional cell cultures, tissues or whole organs. In addition, laser-assisted micromanipulation, e.g. optical tweezers or optoporation techniques, have been revealed as helpful tools for diagnosis or treatment of single cells. In vitro diagnostics often requires methods of (super-resolution) microscopy, hyperspectral imaging or fluorescence lifetime imaging as well as application of opto-acoustic methods, high-content reader systems or optical biosensors. Deconvolution, automated image processing and machine learning have become valuable tools for the evaluation of large data sets. Contributions on all these and related techniques, as well as their application to various fields of cell biology, are welcome in this Special Issue.

Prof. Dr. Christoph Cremer
Prof. Dr. Herbert Schneckenburger
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • light microscopy
  • micromanipulation
  • in vitro diagnostics
  • biomedical laser applications
  • hyperspectral imaging
  • fluorescence lifetime imaging
  • super-resolution
  • high content reader systems
  • biosensors
  • data analysis

Published Papers (3 papers)

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Research

9 pages, 2058 KiB  
Communication
Quartz Crystal Microbalance Platform for SARS-CoV-2 Immuno-Diagnostics
by Per H. Nilsson, Mahmoud Al-Majdoub, Ahmed Ibrahim, Obaidullah Aseel, Subramanian Suriyanarayanan, Linnea Andersson, Samir Fostock, Teodor Aastrup, Ivar Tjernberg, Ingvar Rydén and Ian A. Nicholls
Int. J. Mol. Sci. 2023, 24(23), 16705; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms242316705 - 24 Nov 2023
Viewed by 851
Abstract
Rapid and accurate serological analysis of SARS-CoV-2 antibodies is important for assessing immune protection from vaccination or infection of individuals and for projecting virus spread within a population. The quartz crystal microbalance (QCM) is a label-free flow-based sensor platform that offers an opportunity [...] Read more.
Rapid and accurate serological analysis of SARS-CoV-2 antibodies is important for assessing immune protection from vaccination or infection of individuals and for projecting virus spread within a population. The quartz crystal microbalance (QCM) is a label-free flow-based sensor platform that offers an opportunity to detect the binding of a fluid-phase ligand to an immobilized target molecule in real time. A QCM-based assay was developed for the detection of SARS-CoV-2 antibody binding and evaluated for assay reproducibility. The assay was cross-compared to the Roche electrochemiluminescence assay (ECLIA) Elecsys® Anti-SARS-CoV-2 serology test kit and YHLO’s chemiluminescence immunoassay (CLIA). The day-to-day reproducibility of the assay had a correlation of r2 = 0.99, p < 0.001. The assay linearity was r2 = 0.96, p < 0.001, for dilution in both serum and buffer. In the cross-comparison analysis of 119 human serum samples, 59 were positive in the Roche, 52 in the YHLO, and 48 in the QCM immunoassay. Despite differences in the detection method and antigen used for antibody capture, there was good coherence between the assays, 80–100% for positive and 96–100% for negative test results. In summation, the QCM-based SARS-CoV-2 IgG immunoassay showed high reproducibility and linearity, along with good coherence with the ELISA-based assays. Still, factors including antibody titer and antigen-binding affinity may differentially affect the various assays’ responses. Full article
(This article belongs to the Special Issue Biomedical Optics in Cell Biology)
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18 pages, 6525 KiB  
Article
A Guide to Perform 3D Histology of Biological Tissues with Fluorescence Microscopy
by Annunziatina Laurino, Alessandra Franceschini, Luca Pesce, Lorenzo Cinci, Alberto Montalbano, Giacomo Mazzamuto, Giuseppe Sancataldo, Gabriella Nesi, Irene Costantini, Ludovico Silvestri and Francesco Saverio Pavone
Int. J. Mol. Sci. 2023, 24(7), 6747; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms24076747 - 04 Apr 2023
Cited by 3 | Viewed by 2747
Abstract
The analysis of histological alterations in all types of tissue is of primary importance in pathology for highly accurate and robust diagnosis. Recent advances in tissue clearing and fluorescence microscopy made the study of the anatomy of biological tissue possible in three dimensions. [...] Read more.
The analysis of histological alterations in all types of tissue is of primary importance in pathology for highly accurate and robust diagnosis. Recent advances in tissue clearing and fluorescence microscopy made the study of the anatomy of biological tissue possible in three dimensions. The combination of these techniques with classical hematoxylin and eosin (H&E) staining has led to the birth of three-dimensional (3D) histology. Here, we present an overview of the state-of-the-art methods, highlighting the optimal combinations of different clearing methods and advanced fluorescence microscopy techniques for the investigation of all types of biological tissues. We employed fluorescence nuclear and eosin Y staining that enabled us to obtain hematoxylin and eosin pseudo-coloring comparable with the gold standard H&E analysis. The computational reconstructions obtained with 3D optical imaging can be analyzed by a pathologist without any specific training in volumetric microscopy, paving the way for new biomedical applications in clinical pathology. Full article
(This article belongs to the Special Issue Biomedical Optics in Cell Biology)
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11 pages, 4079 KiB  
Communication
Impact of Doxorubicin on Cell-Substrate Topology
by Andreas Krecsir, Verena Richter, Michael Wagner and Herbert Schneckenburger
Int. J. Mol. Sci. 2022, 23(11), 6277; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms23116277 - 03 Jun 2022
Cited by 1 | Viewed by 1459
Abstract
Variable-Angle Total Internal Reflection Fluorescence Microscopy (VA-TIRFM) is applied in view of early detection of cellular responses to the cytostatic drug doxorubicin. Therefore, we determined cell-substrate topology of cultivated CHO cells transfected with a membrane-associated Green Fluorescent Protein (GFP) in the nanometer range [...] Read more.
Variable-Angle Total Internal Reflection Fluorescence Microscopy (VA-TIRFM) is applied in view of early detection of cellular responses to the cytostatic drug doxorubicin. Therefore, we determined cell-substrate topology of cultivated CHO cells transfected with a membrane-associated Green Fluorescent Protein (GFP) in the nanometer range prior to and subsequent to the application of doxorubicin. Cell-substrate distances increased up to a factor of 2 after 24 h of application. A reduction of these distances by again a factor 2 was observed upon cell aging, and an influence of the cultivation time is presently discussed. Applicability of VA-TIRFM was supported by measurements of MCF-7 breast cancer cells after membrane staining and incubation with doxorubicin, when cell-substrate distances increased again by a factor ≥ 2. So far, our method needs well-defined cell ages and staining of cell membranes or transfection with GFP or related molecules. Use of intrinsic fluorescence or even light-scattering methods to various cancer cell lines could make this method more universal in the future, e.g., in the context of early detection of apoptosis. Full article
(This article belongs to the Special Issue Biomedical Optics in Cell Biology)
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